Expression of transferrin receptors and intracellular ferritin during terminal differentiation of human monocytes

Andreesen, Reinhard; Osterholz, J.; Bodemann, H. H.; Bross, K. J.; Costabel, Ulrich; Löhr, G. W.

doi:10.1007/bf00319822

Cited by 102 publications

(36 citation statements)

References 27 publications

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“…Indeed, CD71, which corresponds to the transferrin receptor, is well known as a macrophagic differentiation marker (31,32). In agreement with this point, monocytic cells, which initially did not express CD71, exhibited a marked expression of this surface marker after 6 days of culture, i.e., when they had acquired a macrophagic phenotype (Fig.…”

Section: Pahs Alter Expression Of Phenotypic Surface Markers In Gm-csmentioning

confidence: 99%

Polycyclic Aromatic Hydrocarbons Inhibit Differentiation of Human Monocytes into Macrophages

Grevenynghe

Rion

Ferrec

et al. 2003

The Journal of Immunology

143

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Polycyclic aromatic hydrocarbons (PAHs) such as benzo(a)pyrene (BP) are ubiquitous environmental carcinogenic contaminants exerting deleterious effects toward cells acting in the immune defense such as monocytic cells. To investigate the cellular basis involved, we have examined the consequences of PAH exposure on macrophagic differentiation of human blood monocytes. Treatment by BP markedly inhibited the formation of adherent macrophagic cells deriving from monocytes upon the action of either GM-CSF or M-CSF. Moreover, it reduced expression of macrophagic phenotypic markers such as CD71 and CD64 in GM-CSF-treated monocytic cells, without altering cell viability or inducing an apoptotic process. Exposure to BP also strongly altered functional properties characterizing macrophagic cells such as endocytosis, phagocytosis, LPS-triggered production of TNF-α and stimulation of allogeneic lymphocyte proliferation. Moreover, formation of adherent macrophagic cells was decreased in response to PAHs distinct from BP such as dimethylbenz(a)anthracene and 3-methylcholanthrene, which interact, like BP, with the arylhydrocarbon receptor (AhR) known to mediate many PAH effects. In contrast, benzo(e)pyrene, a PAH not activating AhR, had no effect. In addition, AhR was demonstrated to be present and functional in cultured monocytic cells, and the use of its antagonist α-naphtoflavone counteracted inhibitory effects of BP toward macrophagic differentiation. Overall, these data demonstrate that exposure to PAHs inhibits functional in vitro differentiation of blood monocytes into macrophages, likely through an AhR-dependent mechanism. Such an effect may contribute to the immunotoxicity of these environmental carcinogens owing to the crucial role played by macrophages in the immune defense.

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Section: Pahs Alter Expression Of Phenotypic Surface Markers In Gm-csmentioning

confidence: 99%

Polycyclic Aromatic Hydrocarbons Inhibit Differentiation of Human Monocytes into Macrophages

Grevenynghe

Rion

Ferrec

et al. 2003

The Journal of Immunology

143

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“…The concept that mononuclear phagocytes modulate the expression of various genes during the maturation process is consistent with the knowledge that the array ofproteins produced by tissue macrophages is both qualitatively and quantitatively different from that of blood monocytes. Included among such proteins are the complement proteins (79,92,93), the transfenin receptor (94)(95)(96), fibronectin (97), interleukin 1 (97), histocompatibility antigens (98), and a growth factor (99).…”

Section: Northern Analysismentioning

confidence: 99%

Expression of the alpha-1-antitrypsin gene in mononuclear phagocytes of normal and alpha-1-antitrypsin-deficient individuals.

Mornex¹,

Chytil-Weir²,

Martinet³

et al. 1986

J. Clin. Invest.

166

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To evaluate the contribution of mononuclear phagocytes, and particularly alveolar macrophages, to alpha-l-antitrypsin (alAT) production in normal and alAT-deficient individuals, Northern analysis with a human alAT complementary DNA was used to demonstrate that alAT messenger RNA (mRNA) can be detected in liver, blood monocytes, and alveolar macrophages.Quantification of alAT mRNA expression demonstrated that: (a) type PiMM monocytes and alveolar macrophages expressed, respectively, 200-fold and 70-fold less alAT mRNA per cell than the liver, (b) the level of expression of the alAT gene was increased during the in vitro maturation of blood monocytes; and (c) blood monocyte and alveolar macrophage levels of expression of the alAT gene were the same in PiMM and PiZZ individuals. However, the amount of newly synthesized alAT secreted by ZZ alveolar macrophages was 10 times lower than that secreted by MM alveolar macrophages. Thus, mononuclear phagocytes of PiZZ individuals express a secretory defect in alAT in a fashion similar to hepatocytes. Not only do mononuclear phagocytes provide a readily accessible cell to evaluate the regulation of alAT gene expression, but these cells may contribute to the levels of alAT present in the lower respiratory tract in the nornul and ZZ states.

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“…Mononuclear phagocytes, like other mammalian cells, acquire iron via transferrin receptors on their surface (13,14). The transferrin receptor is thought to be composed of two identical polypeptide chains, each capable of binding a diferric transferrin molecule at neutral pH (15).…”

Section: Introductionmentioning

confidence: 99%

Interferon gamma-activated human monocytes downregulate transferrin receptors and inhibit the intracellular multiplication of Legionella pneumophila by limiting the availability of iron.

Byrd¹,

Horwitz²

1989

J. Clin. Invest.

299

247

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IntroductionWe have investigated the role of iron in the intracellular biology of Legionella pneumophila in human monocytes and in the effector arm of cell-mediated immune defense against this intracellular bacterial pathogen. By Scatchard analysis of '251-transferrin binding to monocytes, nonactivated monocytes had 38,300±12,700 (mean±SE) transferrin binding sites, whereas activated monocytes had 10,300±1,600, a reduction of 73%. Activated and nonactivated monocytes had a similar mean Kd (1.8±0.2 nM).This study demonstrates that (a) L. pneumophila intracellular multiplication is iron dependent; (b) activated monocytes inhibit L. pneumophila multiplication by limiting the availability of intracellular iron; and (c) transferrin receptors are downregulated on IFNy-activated monocytes.

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Expression of transferrin receptors and intracellular ferritin during terminal differentiation of human monocytes

Cited by 102 publications

References 27 publications

Polycyclic Aromatic Hydrocarbons Inhibit Differentiation of Human Monocytes into Macrophages

Polycyclic Aromatic Hydrocarbons Inhibit Differentiation of Human Monocytes into Macrophages

Expression of the alpha-1-antitrypsin gene in mononuclear phagocytes of normal and alpha-1-antitrypsin-deficient individuals.

Interferon gamma-activated human monocytes downregulate transferrin receptors and inhibit the intracellular multiplication of Legionella pneumophila by limiting the availability of iron.

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