Expression of transfected human CuZn superoxide dismutase gene in mouse L cells and NS20Y neuroblastoma cells induces enhancement of glutathione peroxidase activity

Ceballos, I.; Delabar, Jean–Maurice; Nicole, Annie; Lynch, R.E.; Hallewell, Robert A.; Kamoun, P.; Sinet, P.-M.

doi:10.1016/0167-4781(88)90054-1

Cited by 79 publications

(24 citation statements)

References 28 publications

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“…It is not clear whether the GPX increase is related to a H 2 O 2 increase caused by overexpression of MnSOD. It has been reported that overexpression of CuZnSOD induced an increased GPX activity that might compensate for the increased H 2 O 2 levels (Ceballos et al, 1988;Kelner and Bagnell, 1990;Amstad et al, 1994). It has been hypothesized that increased CuZnSOD may cause oxidative stress to the cells by increasing intracellular concentrations of H 2 O 2 and the elevated GPX may be an adaptive response against the H 2 O 2 cytotoxicity.…”

Section: Discussionmentioning

confidence: 99%

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Suppression of the malignant phenotype of human glioma cells by overexpression of manganese superoxide dismutase

et al. 1997

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Manganese superoxide dismutase (MnSOD) has been previously shown to suppress the malignant phenotype of human melanoma and breast cancer cells. To test the possible role of MnSOD in glioma malignancy, MnSOD was overexpressed in wild type human glioma U118 cells and subcloned U118-9 cells by transfection of human MnSOD cDNA. The MnSOD-transfected cell lines demonstrated expression of exogenous (plasmid) MnSOD mRNA, increase in MnSOD immunoreactive protein, and a three-to eightfold increase in MnSOD enzymatic activity. The MnSOD overexpressing cell lines became less malignant as demonstrated by requiring a higher serum concentration to grow in vitro and much slower tumor growth in nude mice than the parental and neo control cell lines. These ®ndings further support the hypothesis that MnSOD may be a tumor suppressor gene in a wide variety of human tumors.

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Section: Discussionmentioning

confidence: 99%

“…Moreover, transfection of SOD has been associated with changes in the other antioxidant enzymes (Ceballos et al, 1988;Kelner and Bagnell, 1990;Amstad et al, 1994). We therefore performed enzymatic assays to measure the activities of the four major antioxidant enzymes as shown in Table 1.…”

Section: Antioxidant Enzyme Activity In U118 Cellsmentioning

confidence: 99%

Suppression of the malignant phenotype of human glioma cells by overexpression of manganese superoxide dismutase

et al. 1997

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“…5,11,21). Interestingly, at least in some cases where such has been noted, the levels of H202 detoxifying enzymes appear to increase in an adaptive response to the higher H202 levels generated via increased SOD activity (5,11).…”

Section: Resultsmentioning

confidence: 99%

Overproduction of Petunia Chloroplastic Copper/Zinc Superoxide Dismutase Does Not Confer Ozone Tolerance in Transgenic Tobacco

Pitcher¹,

Brennan²,

Hurley³

et al. 1991

Plant Physiol.

129

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Transgenic tobacco (Nicotiana tabacum cultivar W38) plants that overproduce petunia chloroplastic Cu/Zn superoxide dismutase were exposed to ozone dosages that injure control tobacco plants. Based on foliar injury ratings, there was no consistent protection provided to the transgenic plants. These Ozone damage is thought to result from the action of free radicals and other oxidants produced by the interaction of 03 and its degradation products with plant tissue constituents (9). Cellular antioxidant systems are a front-line defense against oxygen free radicals. The activity of SOD,2 one such enzymatic antioxidant, has been shown to increase in response to 03 (7,8,14). Whether this increase is part of a protective, defense response or is a secondary consequence of injury is subject to debate. Lee and Bennett (12) reported a substantial increase in SOD activity in bean plants treated with EDU, a compound capable of conferring tolerance on 03-susceptible plants (4). They also attributed the "natural" tolerance of young versus old leaves in the 03-susceptible BBL290 bean cultivar to a higher endogenous SOD content in young leaves. They suggested that SOD plays a major role in detoxifying 03-induced superoxide radicals which are at least in part responsible for ozone injury.We (200 ,g/ mL). Three plant types were tested: the control W38. 11, which had been transformed with the kanamycin resistance gene only, and independent transformants SOD 35/1.4 and SOD 13.22 (hereafter called SI and S2, respectively), which overproduced the petunia chloroplastic Cu/Zn-SOD (19). Vegetative plants aged 3 to 4 months with 9 to 11 nonsenescent leaves were used, uniform plants being chosen within each experiment. The newest leaf over 2 cm long was designated as leaf 1. Phenotypically there were no distinguishing features between the control and the two SOD transformants.Ozone fumigation tests were conducted with five plants of each genotype in a dynamic flow system contained within a greenhouse (13); an equal number were placed in a similar chamber minus 03. Ozone was produced by a generator (OREC model 0341-0) supplied with high grade oxygen. Ozone concentration in the chamber was measured by the neutral potassium iodide method (10). A dosage of 0.30 ppm 03 for 6 h was used because it was capable of eliciting a differential response from indicator tobacco cultivars Bel W3 and BelB, sensitive and tolerant, respectively. Forty-eight hours after ozonation, leaves 1 through 8 were rated for visible injury. The experiment was conducted three times. Injury data for leaves 5 through 8 were subjected to analysis of www.plantphysiol.org on May 8, 2018 -Published by Downloaded from

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“…In addition to increased SOD activity, many studies have reported increased activity of GSH-Px in various tissues of individuals with DS 6,7,11,13,16,[28][29][30][31][32][33] , as well as in animal models 18 . Because Wijnen and colleagues 34 have localised the gene for GSH-Px on chromosome 3, its increase in individuals with DS is not a gene dose effect.…”

Section: Compensatory Increase In the Activity Of Glutathione Peroxidmentioning

confidence: 99%

“…Similarly, many animal studies have shown 50% or more overexpression of SOD in transgenic mouse models of DS 5,[17][18][19][20] .…”

Section: Increased Superoxide Dismutase (Sod) Activitymentioning

confidence: 99%

Nutritional supplementation in Down syndrome: theoretical considerations and current status

Ani

Grantham‐McGregor

Muller

2000

Develop Med Child Neuro

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Expression of transfected human CuZn superoxide dismutase gene in mouse L cells and NS20Y neuroblastoma cells induces enhancement of glutathione peroxidase activity

Cited by 79 publications

References 28 publications

Suppression of the malignant phenotype of human glioma cells by overexpression of manganese superoxide dismutase

Suppression of the malignant phenotype of human glioma cells by overexpression of manganese superoxide dismutase

Overproduction of Petunia Chloroplastic Copper/Zinc Superoxide Dismutase Does Not Confer Ozone Tolerance in Transgenic Tobacco

Nutritional supplementation in Down syndrome: theoretical considerations and current status

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