Expression of Transcriptional Factor Genes (Oct-4, Nanog, and Sox-2) and Embryonic Stem Cell-Like Characters in Placental Membrane of Buffalo (Bubalus bubalis)

Dev, Kapil; Giri, Shiv Kumar; Kumar, Anil; Yadav, Anita; Singh, Birbal; Gautam, Sanjeev K.

doi:10.1007/s00232-012-9427-5

Cited by 12 publications

(4 citation statements)

References 28 publications

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“…H. pylori extracts were cocultured with the AGS and MKN45 GC cell lines to determine whether it influenced CSC properties. Specific markers could be detected to identify CSC properties, such as CD44, 27 SOX‐2, 28 Nanog, 29 and OCT‐4 30 . In vitro formation of three‐dimensional spheroids highlights the capacity of cells for self‐renewal, which is ideal for the identification of CSC properties.…”

Section: Resultsmentioning

confidence: 99%

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RSPO3 induced by Helicobacter pylori extracts promotes gastric cancer stem cell properties through the GNG7/β‐catenin signaling pathway

Rao,

Zhang,

et al. 2024

Cancer Medicine

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BackgroundHelicobacter pylori (H. pylori) accounts for the majority of gastric cancer (GC) cases globally. The present study found that H. pylori promoted GC stem cell (CSC)‐like properties, therefore, the regulatory mechanism of how H. pylori promotes GC stemness was explored.MethodsSpheroid‐formation experiments were performed to explore the self‐renewal capacity of GC cells. The expression of R‐spondin 3 (RSPO3), Nanog homeobox, organic cation/carnitine transporter‐4 (OCT‐4), SRY‐box transcription factor 2 (SOX‐2), CD44, Akt, glycogen synthase kinase‐3β (GSK‐3β), p‐Akt, p‐GSK‐3β, β‐catenin, and G protein subunit gamma 7 (GNG7) were detected by RT‐qPCR, western blotting, immunohistochemistry (IHC), and immunofluorescence. Co‐immunoprecipitation (CoIP) and liquid chromatography coupled with tandem mass spectrometry (LC–MS/MS) were performed to identify proteins interacting with RSPO3. Lentivirus‐based RNA interference constructed short hairpin (sh)‐RSPO3 GC cells. Small interfering RNA transfection was performed to inhibit GNG7. The in vivo mechanism was verified using a tumor peritoneal seeding model in nude mice.ResultsH. pylori extracts promoted a CSC‐like phenotype in GC cells and elevated the expression of RSPO3. RSPO3 knockdown significantly reduced the CSC‐like properties induced by H. pylori. Previous studies have demonstrated that RSPO3 potentiates the Wnt/β‐catenin signaling pathway, but the inhibitor of Wnt cannot diminish the RSPO3‐induced activation of β‐catenin. CoIP and LC–MS/MS revealed that GNG7 is one of the transmembrane proteins interacting with RSPO3, and it was confirmed that RSPO3 directly interacted with GNG7. Recombinant RSPO3 protein increased the phosphorylation level of Akt and GSK‐3β, and the expression of β‐catenin in GC cells, but this regulatory effect of RSPO3 could be blocked by GNG7 knockdown. Of note, GNG7 suppression could diminish the promoting effect of RSPO3 to CSC‐like properties. In addition, RSPO3 suppression inhibited MKN45 tumor peritoneal seeding in vivo. IHC staining also showed that RSPO3, CD44, OCT‐4, and SOX‐2 were elevated in H. pylori GC tissues.ConclusionRSPO3 enhanced the stemness of H. pylori extracts‐infected GC cells through the GNG7/β‐catenin signaling pathway.

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Section: Resultsmentioning

confidence: 99%

“…Specific markers could be detected to identify CSC properties, such as CD44, 27 SOX-2, 28 Nanog, 29 and OCT-4. 30 In vitro formation of threedimensional spheroids highlights the capacity of cells for self-renewal, which is ideal for the identification of CSC properties.…”

Section: Gc Cells Cocultured With H Pylori Extracts Generates Cells W...mentioning

confidence: 99%

RSPO3 induced by Helicobacter pylori extracts promotes gastric cancer stem cell properties through the GNG7/β‐catenin signaling pathway

Rao,

Zhang,

et al. 2024

Cancer Medicine

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“…They are frequently used as markers for undifferentiated cells. Oct-4, Sox 2, and Nanog positively regulate transcription of all pluripotency circuitry proteins in the leukemia inhibitory factor pathway. , These three factors contribute to a complex molecular network that regulates a number of genes controlling pluripotency. c-MYC is a regulator gene that codes for multifunctional and nuclear phosphoprotein that plays a role in cell cycle progression, apoptosis, and cellular transformation .…”

Section: Resultsmentioning

confidence: 99%

Differentiation of bMSCs on Biocompatible, Biodegradable, and Biomimetic Scaffolds for Largely Defected Tissue Repair

Wang

Jin

Hou

et al. 2019

ACS Appl. Bio Mater.

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Biocompatible, biodegradable, and biomimetic scaffolds in combination with stem cells are of great importance for tissue engineering, especially the repairing and regeneration of defected organs. As a case in point, esophageal diseases have become serious clinical problems because of the poor self-repairing ability of the organ. It is crucial to prepare artificial replacements with biological function for serious lesions of the esophagus. However, the pH value, mechanical strength, thickness, and other physical conditions are very different in different organs or different parts of the same organ, which pose high difficulty for successful tissue engineering. In this work, bone marrow mesenchymal stem cells (bMSCs) were isolated from rabbits and transfected with green fluorescent protein (GFP) to follow their capabilities of growth, stemness, and differentiation in ex vivo culture. These bMSCs were seeded on biocompatible, biodegradable, and biomimetic scaffolds to detect the tissue regenerative capability of the esophagus with multilayer hierarchical structure. According to the esophageal bilayer muscle architecture, we designed discontinuous and continuous microchannel patterned scaffolds with medical level polyurethane (PU) as the matrix to guide the inner-circular and outer-longitudinal muscle growth. The gap on the discontinuous walls not only helped cells to communicate with each other but also assisted cells to infiltrate through the gap and grew into the inner circular muscle. The graft of silk fibroin on the scaffold surface using the aminolysis and glutaraldehyde cross-linking method enhanced the substrate’s hydrophilicity and biocompatibility. Mucosa-submucosa tissue of rabbit’s esophagus was decellularized to obtain the extracellular matrix (ECM) and implanted in situ after recellularizing with bMSCs to repair the partially defected rabbits’ esophagus. On the basis of both in vitro and in vivo results, we concluded that esophagus regeneration was promoted by the differentiation of bMSCs on the biocompatible, biodegradable, and biomimetic scaffolds, starting from tissue “niches”, to repair the largely defected esophagus, which paves the way for tissue engineering and defected organ treatments.

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“…) and buffalo (Dev et al. ,b,c; Mann et al. ), AMSCs expressed NANOG at RT‐PCR, and canine AMSCs were negative (Filioli Uranio et al.…”

Section: Amniotic Membranementioning

confidence: 99%

Stem Cells from Foetal Adnexa and Fluid in Domestic Animals: An Update on Their Features and Clinical Application

Iacono

Rossi

Merlo

2015

Reprod Domestic Animals

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Over the past decade, stem cell research has emerged as an area of major interest for its potential in regenerative medicine applications. This is in constant need of new cell sources to conceive regenerative medicine approaches for diseases that are still without therapy. Scientists drew the attention towards alternative sources such as foetal adnexa and fluid, as these sources possess many advantages: first of all, cells can be extracted from discarded foetal material and it is non-invasive and inexpensive for the patient; secondly, abundant stem cells can be obtained; and finally, these stem cell sources are free from ethical considerations. Cells derived from foetal adnexa and fluid preserve some of the characteristics of the primitive embryonic layers from which they originate. Many studies have demonstrated the differentiation potential in vitro and in vivo towards mesenchymal and non-mesenchymal cell types; in addition, the immune-modulatory properties make these cells a good candidate for allo- and xenotransplantation. Naturally occurring diseases in domestic animals can be more ideal as disease model of human genetic and acquired diseases and could help to define the potential therapeutic use efficiency and safety of stem cells therapies. This review offers an update on the state of the art of characterization of domestic animals' MSCs derived from foetal adnexa and fluid and on the latest findings in pre-clinical or clinical setting of the stem cell populations isolated from these sources.

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Expression of Transcriptional Factor Genes (Oct-4, Nanog, and Sox-2) and Embryonic Stem Cell-Like Characters in Placental Membrane of Buffalo (Bubalus bubalis)

Cited by 12 publications

References 28 publications

RSPO3 induced by Helicobacter pylori extracts promotes gastric cancer stem cell properties through the GNG7/β‐catenin signaling pathway

RSPO3 induced by Helicobacter pylori extracts promotes gastric cancer stem cell properties through the GNG7/β‐catenin signaling pathway

Differentiation of bMSCs on Biocompatible, Biodegradable, and Biomimetic Scaffolds for Largely Defected Tissue Repair

Stem Cells from Foetal Adnexa and Fluid in Domestic Animals: An Update on Their Features and Clinical Application

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