Expression of transcription factors divides retinal ganglion cells into distinct classes

Sweeney, Neal; James, Kiely N.; Nistorica, Andreea; Lorig-Roach, Ryan; Feldheim, David A.

doi:10.1002/cne.24172

Cited by 37 publications

(42 citation statements)

References 58 publications

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“…In the retina (Figure k,l) the ganglion cell layer (GaCL) contained scattered nG+ nuclei (plus rare nG+ nuclei in the inner nuclear layer (INL), presumably displaced ganglion cells), while mT fluorescence was enriched in the plexiform layers (IPL and OPL) and optic nerve (Figure l, arrow). These observations in the retina matched descriptions of Eomes expression in non‐image‐forming visual pathways (Mao et al, ; Sweeney, James, Nistorica, Lorig‐Roach, & Feldheim, ; Sweeney, Tierney, & Feldheim, ). Together, these observations support faithful mTnG reporter expression in Eomes + cells of the CNS, and indicate that the allele can serve as a lineage tracer for periods of days to weeks in postmitotic neuronal cell types derived from EOMES+ progenitors.…”

Section: Resultssupporting

confidence: 80%

“…Also in P8 mice, the posterior periventricle (pPV), previously identified as neurogenic source after brain injury (Nakatomi et al, 2002), was rich in nG1 cells (Figure 4h,i), confirming previous observations that the pPV is rich in Eomes-expressing progenitors (Kowalczyk et al, 2009). The dentate gyrus (Figure 4j Sweeney, James, Nistorica, Lorig-Roach, & Feldheim, 2017;Sweeney, Tierney, & Feldheim, 2014). Together, these observations support faithful mTnG reporter expression in Eomes1 cells of the CNS, and indicate that the allele can serve as a lineage tracer for periods of days to weeks in postmitotic neuronal cell types derived from EOMES1 progenitors.…”

Section: Also Cells Of the Outer Ve Layer Show Reporter Expression (supporting

confidence: 54%

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A dual‐fluorescence reporter in the Eomes locus for live imaging and medium‐term lineage tracing

Probst

Daza²,

Bader

et al. 2017

Genesis

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The T-box transcription factor Eomes (also known as Tbr2) shows short-lived expression in various localized domains of the embryo, including epiblast cells during gastrulation and intermediate progenitor cells in the cerebral cortex. In these tissues Eomes fulfills crucial roles for lineage specification of progenitors. To directly observe Eomes-dependent cell lineages in the living embryo, we generated a novel dual-fluorescence reporter allele that expresses a membrane-bound tdTomato protein for investigation of cell morphology and a nuclear GFP for cell tracing. This allele recapitulates endogenous EOMES protein expression and is suitable for live imaging. We found that the allele can also be used as a short-to-medium-term lineage tracer, as GFP persists in cells longer than EOMES protein and marks Eomes-dependent lineages with a timeframe of days to weeks depending on the proliferation rate. In summary, we present a novel genetic tool for investigation of Eomes-dependent cell types by live imaging and lineage tracing.

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Section: Resultssupporting

confidence: 80%

Section: Also Cells Of the Outer Ve Layer Show Reporter Expression (supporting

confidence: 54%

A dual‐fluorescence reporter in the Eomes locus for live imaging and medium‐term lineage tracing

Probst

Daza²,

Bader

et al. 2017

Genesis

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“…C. Mouse HCs are transcriptionally more similar to macaque H1 (Figure 5B) (Cherry et al, 2011;Liu et al, 2018;Mao et al, 2014;Peng et al, 2017;Rousso et al, 2016;Sweeney et al, 2017).…”

Section: Figure S3: Histological Validation Of Markers For Rgcsmentioning

confidence: 97%

Molecular Classification and Comparative Taxonomics of Foveal and Peripheral Cells in Primate Retina

Peng

Shekhar

Yan

et al. 2018

Preprint

133

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High acuity vision in primates, including humans, is mediated by a small central retinal region called the fovea. As more accessible model organisms lack a fovea, its specialized function and dysfunction in ocular diseases remain poorly understood. We used 165,000 single-cell RNA-seq profiles to generate and validate comprehensive cellular taxonomies of macaque fovea and peripheral retina. More than 80% of >65 cell types match between the two regions, but exhibit substantial differences in proportions and gene expression, some of which we relate to functional differences. Comparison of macaque retinal types with those of mice reveals that interneuron types are tightly conserved, but that projection neuron types and programs diverge, despite conserved transcription factor codes. Key macaque types are conserved in humans, allowing mapping of cell-type and region-specific expression of >190 genes associated with 6 human retinal diseases. Our work provides a framework for comparative single-cell analysis across tissue regions and species. $00&;789: 4789:

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“…Last but not least, countless studies have classified RGCs following their molecular signature, and in particular, the presence of determining transcription factors. Recently, Sweeney et al have proposed that almost all RGCs could be categorized in three classes depending on their expression of Isl2, Tbr2, or Satb1/Satb2 and hypothesized that these TFs are responsible for RGC functional identity [178]. Supporting this idea, Isl2 colocalizes with Smi32 a marker of alpha-RGCs [179].…”

Section: Molecular Criteriamentioning

confidence: 98%

Neurogenesis and Specification of Retinal Ganglion Cells

Nguyen-Ba-Charvet

Rebsam

2020

IJMS

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Across all species, retinal ganglion cells (RGCs) are the first retinal neurons generated during development, followed by the other retinal cell types. How are retinal progenitor cells (RPCs) able to produce these cell types in a specific and timely order? Here, we will review the different models of retinal neurogenesis proposed over the last decades as well as the extrinsic and intrinsic factors controlling it. We will then focus on the molecular mechanisms, especially the cascade of transcription factors that regulate, more specifically, RGC fate. We will also comment on the recent discovery that the ciliary marginal zone is a new stem cell niche in mice contributing to retinal neurogenesis, especially to the generation of ipsilateral RGCs. Furthermore, RGCs are composed of many different subtypes that are anatomically, physiologically, functionally, and molecularly defined. We will summarize the different classifications of RGC subtypes and will recapitulate the specification of some of them and describe how a genetic disease such as albinism affects neurogenesis, resulting in profound visual deficits.

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Expression of transcription factors divides retinal ganglion cells into distinct classes

Cited by 37 publications

References 58 publications

A dual‐fluorescence reporter in the Eomes locus for live imaging and medium‐term lineage tracing

A dual‐fluorescence reporter in the Eomes locus for live imaging and medium‐term lineage tracing

Molecular Classification and Comparative Taxonomics of Foveal and Peripheral Cells in Primate Retina

Neurogenesis and Specification of Retinal Ganglion Cells

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