Expression of Toll-like receptors and avian β-defensins and their changes in response to bacterial components in chicken sperm

Das, S. C.; Isobe, Naoki; Yoshimura, Y.

doi:10.3382/ps.2010-00850

Cited by 30 publications

(18 citation statements)

References 36 publications

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“…Male chicken seminal fluid contains proteins identified as participating in defence and immunity processes [18, 19], also observed in our previous unpublished results. Chicken semen expresses different types of β-defensins, apparently to protect spermatozoa from microbial damage [47]. As well, TGF-β isoforms known to coat the surface of human spermatozoa [42], elicit changes in the UVJ of turkey hens [10].…”

Section: Discussionmentioning

confidence: 99%

Conserved gene expression in sperm reservoirs between birds and mammals in response to mating

et al. 2017

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BackgroundSpermatozoa are stored in the oviductal functional sperm reservoir in animals with internal fertilization, including zoologically distant classes such as pigs or poultry. They are held fertile in the reservoir for times ranging from a couple of days (in pigs), to several weeks (in chickens), before they are gradually released to fertilize the newly ovulated eggs. It is currently unknown whether females from these species share conserved mechanisms to tolerate such a lengthy presence of immunologically-foreign spermatozoa. Therefore, global gene expression was assessed using cDNA microarrays on tissue collected from the avian utero-vaginal junction (UVJ), and the porcine utero-tubal junction (UTJ) to determine expression changes after mating (entire semen deposition) or in vivo cloacal/cervical infusion of sperm-free seminal fluid (SF)/seminal plasma (SP).ResultsIn chickens, mating changed the expression of 303 genes and SF-infusion changed the expression of 931 genes, as compared to controls, with 68 genes being common to both treatments. In pigs, mating or SP-infusion changed the expressions of 1,722 and 1,148 genes, respectively, as compared to controls, while 592 genes were common to both treatments. The differentially expressed genes were significantly enriched for GO categories related to immune system functions (35.72-fold enrichment). The top 200 differentially expressed genes of each treatment in each animal class were analysed for gene ontology. In both pig and chicken, an excess of genes affecting local immune defence were activated, though frequently these were down-regulated. Similar genes were found in both the chicken and pig, either involved in pH-regulation (SLC16A2, SLC4A9, SLC13A1, SLC35F1, ATP8B3, ATP13A3) or immune-modulation (IFIT5, IFI16, MMP27, ADAMTS3, MMP3, MMP12).ConclusionDespite being phylogenetically distant, chicken and pig appear to share some gene functions for the preservation of viable spermatozoa in the female reservoirs.Electronic supplementary materialThe online version of this article (doi:10.1186/s12864-017-3488-x) contains supplementary material, which is available to authorized users.

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Section: Discussionmentioning

confidence: 99%

Conserved gene expression in sperm reservoirs between birds and mammals in response to mating

et al. 2017

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“…Strangely, other AvBDs (AvBD5, AvBD9, AvBD10, and AvBD12) were also quickly upregulated (4 h) in chicken sperm after LPS incubation (Das et al 2011). Although all TLR signaling pathways culminate in activation of the transcription factor NFkB, which controls the expression of an array of inflammatory cytokine genes, the kinetics of gene expression of NFkB-dependent genes are not activated simultaneously even if cell type and stimulus are constant.…”

Section: Discussionmentioning

confidence: 99%

“…Data on expression analysis presented in this study revealed that all ten members of the cTLR family, nine members of the AvBD family, as well as eight cytokines were expressed in the rooster SCs. Previous studies have reported, in the rooster reproductive tract, the expression of most members of the TLR and AvBD families in the testis, epididymis, and sperm, while S. enteritidis infection or LPS stimulation resulted in significant induction of several of these genes in the testis, epididymis, and sperm of sexually mature roosters (Shimizu et al 2008, Anastasiadou et al 2011, 2014, Das et al 2011, Watanabe et al 2011, Zhang et al 2012. Furthermore, administration of LPS upregulated the expression of IL1b, IL6, and CXCLi2 in the rooster testis and epididymis by 3-6 h after injection (Zhang et al 2012).…”

Section: Discussionmentioning

confidence: 99%

“…We have recently reported that several members of the TLRs family were significantly upregulated in the testis of sexually mature roosters infected with Salmonella enteritidis (Anastasiadou et al 2011). It has also been reported that several members of the avian b-defensin (AvBDs) and TLR families were expressed in chicken sperm and the expression of four AvBDs was significantly increased in the sperm stimulated with LPS (Das et al 2011).…”

Section: Introductionmentioning

confidence: 99%

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Activation of innate immune system in response to lipopolysaccharide in chicken Sertoli cells

Michailidis¹,

Anastasiadou²,

Guibert³

et al. 2014

Reproduction

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Sertoli cells (SCs) play an important physiological role in the testis, as they support, nourish, and protect the germ cells. As protection of the developing spermatozoa is an emerging aspect of reproductive physiology, this study examined the expression pattern of innate immune-related genes, including avian b-defensins (AvBDs), Toll-like receptors (TLRs), and cytokines, and investigated the time course of an inflammatory response in rooster SCs triggered by exposure to the bacterial endotoxin lipopolysaccharide (LPS). SCs were isolated from 6-week-old chicken, cultured in vitro, and stimulated with 1 mg/ml LPS at different time courses (0, 6, 12, 24, and 48 h). Data on expression analysis revealed that all ten members of the chicken TLR family, nine members of the AvBD family, as well as eight cytokine genes were expressed in SCs. Quantitative real-time PCR analysis revealed that LPS treatment resulted in significant induction of the expression levels of six TLRs, six AvBDs, and four cytokine genes, while two cytokine genes were downregulated and two other genes were unchanged. The increasing interleukin 1b (IL1b) production was confirmed in the conditioned medium. Furthermore, the phagocytosis of SCs was increased after LPS treatment. In conclusion, these findings provide evidence that SCs express innate immunerelated genes and respond directly to bacterial ligands. These genes represent an important component of the immune system, which could be integrated into semen, and present a distinctive constituent of the protective repertoire of the testis against ascending infections.

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“…Among the identified proteins, ovotransferrin was strongly expressed while gallinacin-9 was moderately expressed. In Gallus gallus, ovotransferrin was previously reported to be expressed in the oviduct and egg, and gallinacin-9 was expressed in both female and male reproductive organs and the spermatozoa (Das et al, 2011;Gautron et al, 2001;Michailidis and Avdi, 2011).…”

Section: Incubation and Fertility Research Groupmentioning

confidence: 96%

Incubation and Fertility Research Group {WPSA Working Group 6 (Reproduction)} 2011 Meeting - Ede, the Netherlands, 30th-31st August 2011

2011

Avian Biology Research

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Speakers from all over the world took part and considered all aspects of incubation and fertility in a variety of avian species. We are delighted to present abstracts from 27 of the presentations given at the meeting. The topics include: improving the fertility of cryopreserved poultry semen; sexing of day zero unincubated chicken eggs; hypoxia effects on embryonic development; the effects of thermal manipulation during early embryogenesis; antimicrobial activity of egg white; egg size variation in double yolked duck eggs; treatments to preserve hatchability during storage; and the question of how to engineer the heart of broiler chickens during incubation.

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Expression of Toll-like receptors and avian β-defensins and their changes in response to bacterial components in chicken sperm

Cited by 30 publications

References 36 publications

Conserved gene expression in sperm reservoirs between birds and mammals in response to mating

Conserved gene expression in sperm reservoirs between birds and mammals in response to mating

Activation of innate immune system in response to lipopolysaccharide in chicken Sertoli cells

Incubation and Fertility Research Group {WPSA Working Group 6 (Reproduction)} 2011 Meeting - Ede, the Netherlands, 30th-31st August 2011

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