Expression of TNF-α and IL-1β in Peripheral Blood of Patients with T2DM Retinopathy

Abstract

Aims. The expression and clinical significance of tumor necrosis factor-α (INF-α) and interleukin 1-β (IL-1β) in retinal cells of patients with type 2 diabetes (T2DM) retinopathy were detected by flow cytometry. Materials and Methods. Fifty patients with T2DM who attended our ophthalmology clinic between May 2021 and May 2022 were selected as the observation group. Another 50 healthy individuals who were examined at our hospital during the same period were selected as the comparison group. Tear film rupture time (BUT), fluorescein staining (FL), basal tear secretion (Schirmer I) test, and conjunctival impression cytology (CIC) were detected in both groups, and the expression of TNF-α and IL-1β in retinal cells was observed by immunohistochemical staining. Results. The levels of IL13 and TNF-α in the two groups were not exactly the same. The serum levels of IL13 and TNF-α in the observation group were significantly higher than those in the control group, and there was a statistically significant difference ( P < 0.05 ). TNF-α and IL-1B expressions in the observation group were positively correlated with the fluorescence staining, and the expression of TNF-α and IL-1β in the observation group was significantly negatively correlated with the BUT test and Schirmer I test. Conclusion. Serums TNF-α and IL-1β are significantly elevated in patients with T2DM retinopathy and gradually increase with disease progression. Combined detection of serums TNF-α and IL-1β can help determine the severity of the disease and assess the prognosis.

Methylation of the CpG at -299 bp from transcription start site of interleukin-1β is a sustained marker of inflammation exerted by TNFa on bone marrow-derived mesenchymal cells of healthy humans

“…It also exempli es the usage of previously developed procedures to determine CpG methylation [26]. As IL1B expression is used as a marker in clinics [21,[39][40][41], there is a particular signi cance of an approach studing DNA associated marks in addition to RNA expression. Since DNA is more stable, used approach to studying DNA methylation simpli es procedures and allows to analyse stored samples with potential RNA degradation [42][43][44].…”