Expression of tissue inhibitor of metalloproteinase-4 (TIMP-4) in endometrium and placenta of rhesus monkey (Macaca mulatta) during early pregnancy

Yang, Qing; Wang, Hongxing; Zhao, Yunge; Lin, Haiyan; Zhang, Heng; Wang, Hongmei; Sang, Qing‐Xiang Amy; Zhu, Cheng

doi:10.1016/j.lfs.2005.11.016

Cited by 9 publications

(10 citation statements)

References 40 publications

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“…(Hammani et al 1996;Karagiannis and Popel 2004;Melendez-Zajgla et al 2008;Wisniewska et al 2008). Our previous work has implicated that TIMP-1 and -2 are involved in rhesus monkey trophoblast cell invasion and the development of a placenta while TIMP-3 participates in placental angiogenesis (Wang et al 2001;Yang et al 2006;Zhang et al 2004). In this study, we found that PPM1A knockdown down-regulated the expression of TIMP-2 message RNA and protein.…”

Section: Discussionsupporting

confidence: 50%

“…TIMPs form complex with metalloproteinases such as collagenases, gelatinases, and stromelysins, resulting in irreversible inactivation of the metalloproteinases. Coexpression of MMPs and TIMPs in trophoblast suggests that the invasive property of CTB depends on the balance between MMPs and TIMPs (Cohen et al 2006;Yang et al 2006). Reduction of TIMP expression leads to enhanced ECM proteolysis, consistent with up-regulation of cell invasive activity of cells.…”

Section: Discussionmentioning

confidence: 87%

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Protein phosphatase 1A (PPM1A) is involved in human cytotrophoblast cell invasion and migration

Zhang

Zhou

Lin

et al. 2009

Histochem Cell Biol

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Trophoblast invasion is crucial for embryo implantation and placentation. Excessive trophoblast invasion leads to hydatidiform moles and choriocarcinoma. PPM1A is a phosphatase which dephosphorylates and inactivates a broad range of substrates, including TGF-beta, MAP kinases, p38 and JNK kinase cascades, and is involved in tumor suppression. The objective of this study was to investigate the expression of PPM1A in normal and malignant human placenta and its role in trophoblast invasion, which shares many similarities with invasion of tumor cells. By Western blotting and immunocytochemistry, significantly higher expression of PPM1A in human placental villi at term was found as compared with that during the first trimester. Furthermore, the expression level of PPM1A protein in hydatidiform moles was lower compared with that during normal pregnancy. We further investigated the function of PPM1A in extravillous trophoblast cell line HTR8/SVneo. Transwell migration and Matrigel invasion assays demonstrated that PPM1A siRNA significantly promoted the motility and invasiveness of the cells. Gelatin zymography showed that knockdown of PPM1A with siRNA elevated the expression of pro-matrix metalloproteinase pro-(MMP)-9, but down-regulated tissue inhibitors of metalloproteinases (TIMP)-2. The present data indicate that PPM1A plays a critical role in the regulation of normal placentation by inhibiting trophoblast migration and invasion.

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Section: Discussionsupporting

confidence: 50%

Section: Discussionmentioning

confidence: 87%

Protein phosphatase 1A (PPM1A) is involved in human cytotrophoblast cell invasion and migration

Zhang

Zhou

Lin

et al. 2009

Histochem Cell Biol

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“…Second, a matrix metalloproteinase and a matrix metalloproteinase inhibitor ( Mmp15 and Timp4 ) were both positively selected in H. formosa . Both proteins are involved in endometrial tissue remodelling and placental labyrinth formation [31, 32]. Third, Rbl1 and Kiaa1324 gene expression has been linked to endometrial cell proliferation [33, 34].…”

Section: Resultsmentioning

confidence: 99%

“…For a number of genes found to be positively selected in H. formosa it is possible they play a role in this change in tissue structure, most notably Mmp15 and Timp4 . Matrix metalloproteinases and their inhibitors are responsible for tissue remodelling [58], and both Mmp15 and Timp4 are active in the mammalian placenta [31, 32]. Therefore, it is plausible that positive selection acting on these genes could result in a difference in placental morphology.…”

Section: Discussionmentioning

confidence: 99%

The genome of the live-bearing fish Heterandria formosa implicates a role of conserved vertebrate genes in the evolution of placental fish

Kruistum

Heuvel²,

Travis

et al. 2019

BMC Evol Biol

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Background The evolution of complex organs is thought to occur via a stepwise process, each subsequent step increasing the organ’s complexity by a tiny amount. This evolutionary process can be studied by comparing closely related species that vary in the presence or absence of their organs. This is the case for the placenta in the live-bearing fish family Poeciliidae, as members of this family vary markedly in their ability to supply nutrients to their offspring via a placenta. Here, we investigate the genomic basis underlying this phenotypic variation in Heterandria formosa , a poeciliid fish with a highly complex placenta. We compare this genome to three published reference genomes of non-placental poeciliid fish to gain insight in which genes may have played a role in the evolution of the placenta in the Poeciliidae. Results We sequenced the genome of H. formosa , providing the first whole genome sequence for a placental poeciliid. We looked for signatures of adaptive evolution by comparing its gene sequences to those of three non-placental live-bearing relatives. Using comparative evolutionary analyses, we found 17 genes that were positively selected exclusively in H. formosa , as well as five gene duplications exclusive to H. formosa . Eight of the genes evolving under positive selection in H. formosa have a placental function in mammals, most notably endometrial tissue remodelling or endometrial cell proliferation. Conclusions Our results show that a substantial portion of positively selected genes have a function that correlates well with the morphological changes that form the placenta of H. formosa , compared to the corresponding tissue in non-placental poeciliids. These functions are mainly endometrial tissue remodelling and endometrial cell proliferation. Therefore, we hypothesize that natural selection acting on genes involved in these functions plays a key role in the evolution of the placenta in H. formosa . Electronic supplementary material The online version of this article (10.1186/s12862-019-1484-2) contains supplementary material, which is available to authorized users.

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“…143 TIMP-4 might play an important role in glandular secretion, endometrial tissue remodeling, and invasion of the trophoblast cells by regulating MMPs in a localized manner in the uteri of rhesus monkey during early pregnancy. 144 The information available for TIMP-4 provided a putative structural model to propose potential relevant directions toward solving its function and role in diseases such as cancer. 123 Results demonstrated upregulation of TIMP-4 in human cardiovascular disorders exhibiting inflammation.…”

Section: Timp-4mentioning

confidence: 99%

Matrix metalloproteinases – an overview

Sekhon¹

2010

RRB

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Matrix metalloproteinases (MMPs, matrixins) are a family of secreted and membrane-bound zinc-dependent endopeptidases that have the combined capacity to degrade all the components of the extracellular matrix. These enzymes have a common zinc-binding motif (HEXXHXXGXXH) in their active site and a conserved methionine turn following the active site. MMP enzymes are strongly involved in a kaleidoscope of normal, pathological, physiological, and biological processes such as embryogenesis, normal tissue remodeling, wound healing, and angiogenesis, and in diseases such as atheroma, arthritis, cancer, and tissue ulceration. MMPs play a significant role in vascular remodeling. Broad-spectrum metalloproteinase inhibitors as potential therapeutics have been developed to explore the involvement of MMPs in various diseases.

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Expression of tissue inhibitor of metalloproteinase-4 (TIMP-4) in endometrium and placenta of rhesus monkey (Macaca mulatta) during early pregnancy

Cited by 9 publications

References 40 publications

Protein phosphatase 1A (PPM1A) is involved in human cytotrophoblast cell invasion and migration

Protein phosphatase 1A (PPM1A) is involved in human cytotrophoblast cell invasion and migration

The genome of the live-bearing fish Heterandria formosa implicates a role of conserved vertebrate genes in the evolution of placental fish

Matrix metalloproteinases – an overview

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Expression of tissue inhibitor of metalloproteinase-4 (TIMP-4) in endometrium and placenta of rhesus monkey (Macaca mulatta) during early pregnancy

Cited by 9 publications

References 40 publications

Protein phosphatase 1A (PPM1A) is involved in human cytotrophoblast cell invasion and migration

Protein phosphatase 1A (PPM1A) is involved in human cytotrophoblast cell invasion and migration

The genome of the live-bearing fish Heterandria formosa implicates a role of conserved vertebrate genes in the evolution of placental fish

Matrix metalloproteinases &ndash; an overview

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Matrix metalloproteinases – an overview