Expression of tight-junction proteins in human proximal small intestinal mucosa before and after Roux-en-Y gastric bypass surgery

Casselbrant, Anna; Elias, Erik; Fändriks, Lars; Wallenius, Ville

doi:10.1016/j.soard.2014.05.009

Cited by 48 publications

(35 citation statements)

References 40 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…However, research into gut permeability and gut tight junction proteins after RYGB surgery is limited, and some conclusions are debatable. Casselbrant et al found that jejunal claudin‐3 protein expression increased after RYGB and was correlated with the permeability measured using Ussing chambers. Steensels et al reported similar findings, showing that intestinal permeability is reduced independent of reductions in food intake, as determined by oral fluorescein.…”

Section: Discussionmentioning

confidence: 98%

Roux‐en‐Y gastric bypass decreases endotoxemia and inflammatory stress in association with improvements in gut permeability in obese diabetic rats

Guo

Liu

et al. 2019

Journal of Diabetes

View full text Add to dashboard Cite

Background Postoperative modulation of the gut microbiome has been suggested to contribute to the metabolic benefits after metabolic surgery, but the mechanisms underlying these metabolic benefits remain unknown. Previously, we reported that Roux‐en‐Y gastric bypass (RYGB) surgery in Zucker diabetic fatty (ZDF) rats increased the abundance of Proteobacteria and Gammaproteobacteria. However, theoretically, these Gram‐negative bacteria may elevate lipopolysaccharide (LPS) levels. Therefore, in this study we further investigated the potential mechanisms by which RYGB improves glucose homeostasis, endotoxemia, and inflammatory stress in ZDF rats. Methods Rats were divided into three groups: (a) an RYGB group (RY); (b) a sham‐operated group pair‐fed with the RY group; and (c) a sham‐operated group fed ad libitum. Changes in LPS, cytokine levels, intestinal permeability (evaluated using the fluorescein isothiocyanate‐dextran method), and intestinal epithelial tight junction proteins zona occludins (ZO)‐1, occludin, and claudin‐1 were assessed 10 weeks postoperatively. Results Rats that underwent RYGB exhibited sustained weight loss and reduced glucose, as well as lower cytokine and LPS concentrations, than rats in the control groups. In the colonic epithelium, ZO1 and claudin‐1 (Cldn1) mRNA levels were higher in the RY than control groups. Intestinal permeability declined in the RY group and was positively correlated with LPS levels and negatively correlated with ZO‐1, occludin, and claudin‐1 expression. Conclusions The results demonstrate that RYGB can reduce the extent of endotoxemia and inflammation, which is associated with improved tight junction integrity and intestinal barrier strength. These effects may explain why a low level of inflammation is maintained after RYGB and the postoperative increase in Gram‐negative bacteria.

show abstract

Section: Discussionmentioning

confidence: 98%

Roux‐en‐Y gastric bypass decreases endotoxemia and inflammatory stress in association with improvements in gut permeability in obese diabetic rats

Guo

Liu

et al. 2019

Journal of Diabetes

View full text Add to dashboard Cite

show abstract

“…One plausible mechanism is an elevated uptake of short-chain fatty acids in the colon due, in part, to the elevated nutrient intake. Prior investigations have also reported that obesity is associated with elevations in the “leakiness” of the gastrointestinal tract [21,22], which could lead to elevated uptake of short-chain fatty acids through passive uptake, bypassing the short-chain fatty acid transporters altogether. Interestingly, the higher serum concentrations of the short-chain fatty acids were positively correlated with the mRNA abundance of the short-chain fatty acid transporter SMCT1 in the jejunum even though the jejunum is not the primary site of short-chain fatty acid uptake [23].…”

Section: Discussionmentioning

confidence: 99%

Nutrient Transporter Expression in the Jejunum in Relation to Body Mass Index in Patients Undergoing Bariatric Surgery

et al. 2016

View full text Add to dashboard Cite

Nutrient tranters (NT) facilitate nutrient absorption and contribute to the regulation of circulating nutrients. In this cross-sectional study, we determined the associations between the level of obesity; mRNA abundance for NTs; and serum concentrations of amino acids, short-chain fatty acids, and glucose in patients with morbid obesity undergoing a Roux-en-Y gastric bypass. Proximal jejunal samples were obtained at the time of surgery from 42 patients (90% female, age = 42.6 ± 11.9 years, pre-operative body mass index (BMI) = 55.5 ± 11.3 kg/m2) undergoing a Roux-en-Y gastric bypass. RNA was extracted from the jejunal mucosa and quantitative real-time–PCR was performed for the NTs studied. BMI negatively correlated with jejunal mRNA abundance of the amino acid NTs TauT (r = −0.625, p < 0.0001), ASCT2 (r = −0.320, p = 0.039), LAT1 (r = −0.304, p = 0.05). BMI positively correlated with jejunal mRNA abundance of the lactate/short-chain fatty acid NT SMCT1 (r = 0.543, p = 0.0002). Serum concentrations of the short-chain fatty acids, butyric, valeric, and isocaproic acid correlated positively with BMI (n = 30) (r = 0.45, r = 0.44, r = 0.36, p ≤ 0.05; respectively). Lower jejunal mRNA abundance for the amino acid NTs TauT, ASCT2, and LAT1 could protect against further obesity-related elevations in circulating amino acids. The positive correlation between BMI and the jejunal mRNA abundance of the high-affinity short-chain fatty acid/monocarboxylate transporter SMCT1 is intriguing and requires further investigation.

show abstract

“…In addition, several alterations are found in the rerouted small intestine, such as, for example, decreased jejunal mucosal surface area and decreased paracellular permeability. These changes could contribute to a reduction in absorption of luminal microbiota-derived inflammatory mediators, such as endotoxins [9].…”

Section: Indication Of Bariatric Surgery (Roux-en-y Gastric Bypass Anmentioning

confidence: 99%

Indication of Bariatric Surgery (Roux-en-Y Gastric Bypass Surgery and Vertical Gastrectomy) in The Older Individuals and implications For Gut Microbiota

Gottlieb¹,

Junges²,

Closs³

2017

JGPLD

View full text Add to dashboard Cite

Expression of tight-junction proteins in human proximal small intestinal mucosa before and after Roux-en-Y gastric bypass surgery

Cited by 48 publications

References 40 publications

Roux‐en‐Y gastric bypass decreases endotoxemia and inflammatory stress in association with improvements in gut permeability in obese diabetic rats

Roux‐en‐Y gastric bypass decreases endotoxemia and inflammatory stress in association with improvements in gut permeability in obese diabetic rats

Nutrient Transporter Expression in the Jejunum in Relation to Body Mass Index in Patients Undergoing Bariatric Surgery

Indication of Bariatric Surgery (Roux-en-Y Gastric Bypass Surgery and Vertical Gastrectomy) in The Older Individuals and implications For Gut Microbiota

Contact Info

Product

Resources

About