Expression of Tight Junction Protein Claudin-1 in Human Crescentic Glomerulonephritis

Koda, Ryo; Yoshino, Atsunori; Imanishi, Yuji; Kawamoto, Shinya; Ueda, Yoshihiko; Yaoita, Eishin; Kazama, Junichiro James; Narita, Ichiei; Takeda, Takeshi

doi:10.1155/2014/598670

Cited by 10 publications

(7 citation statements)

References 30 publications

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“…The claudin-1 antibody was raised against a synthetic peptide within human claudin-1 aa 150 to the C-terminus. The ab15098 antibody was used for targeting peripheral nerve perineurium ( Cohnen et al, 2020 ), as well as for applications of IHC of many other paraffin-embedded tissues [e.g., skin ( Kim et al, 2016 ), intestine ( Gumber et al, 2014 ; Mandle et al, 2019 ), and kidney ( Koda et al, 2014 )]; other claudin-1 antibodies were also used to label perineurium in peripheral nerves ( Pummi et al, 2004 ; Rittner et al, 2012 ; Piña et al, 2015 ; Reinhold et al, 2018 ).…”

Section: Methodsmentioning

confidence: 99%

Quantified Morphology of the Cervical and Subdiaphragmatic Vagus Nerves of Human, Pig, and Rat

et al. 2020

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It is necessary to understand the morphology of the vagus nerve (VN) to design and deliver effective and selective vagus nerve stimulation (VNS) because nerve morphology influences fiber responses to electrical stimulation. Specifically, nerve diameter (and thus, electrode-fiber distance), fascicle diameter, fascicular organization, and perineurium thickness all significantly affect the responses of nerve fibers to electrical signals delivered through a cuff electrode. We quantified the morphology of cervical and subdiaphragmatic VNs in humans, pigs, and rats: effective nerve diameter, number of fascicles, effective fascicle diameters, proportions of endoneurial, perineurial, and epineurial tissues, and perineurium thickness. The human and pig VNs were comparable sizes (∼2 mm cervically; ∼1.6 mm subdiaphragmatically), while the rat nerves were ten times smaller. The pig nerves had ten times more fascicles—and the fascicles were smaller—than in human nerves (47 vs. 7 fascicles cervically; 38 vs. 5 fascicles subdiaphragmatically). Comparing the cervical to the subdiaphragmatic VNs, the nerves and fascicles were larger at the cervical level for all species and there were more fascicles for pigs. Human morphology generally exhibited greater variability across samples than pigs and rats. A prior study of human somatic nerves indicated that the ratio of perineurium thickness to fascicle diameter was approximately constant across fascicle diameters. However, our data found thicker human and pig VN perineurium than those prior data: the VNs had thicker perineurium for larger fascicles and thicker perineurium normalized by fascicle diameter for smaller fascicles. Understanding these differences in VN morphology between preclinical models and the clinical target, as well as the variability across individuals of a species, is essential for designing suitable cuff electrodes and stimulation parameters and for informing translation of preclinical results to clinical application to advance the therapeutic efficacy of VNS.

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Section: Methodsmentioning

confidence: 99%

Quantified Morphology of the Cervical and Subdiaphragmatic Vagus Nerves of Human, Pig, and Rat

et al. 2020

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“…Nevertheless, their exciting results encourage further investigation of SIRT1 and claudin-1 as therapeutic targets of DN. Furthermore, there was also a study suggesting that claudin-1 contributed to crescent formation in crescentic glomerulonephritis (30). Thus, future studies are needed to investigate whether SIRT1, as a negative regulator of claudin-1, can serve as a potential target to prevent or treat crescentic glomerulonephritis.…”

Section: Sirt1 Preserves Podocyte Function By Targeting Claudin-1mentioning

confidence: 99%

Sirtuin 1: A Target for Kidney Diseases

Kong

Zhou

et al. 2015

Mol Med

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Sirtuin 1 (SIRT1) is an evolutionarily conserved NAD + -dependent histone deacetylase that is necessary for caloric restriction-related lifespan extension. SIRT1, as an intracellular energy sensor, detects the concentration of intracellular NAD + and uses this information to adapt cellular energy output to cellular energy requirements. Previous studies on SIRT1 have confirmed its beneficial effects on cellular immunity to oxidative stress, reduction of fibrosis, suppression of inflammation, inhibition of apoptosis, regulation of metabolism, induction of autophagy and regulation of blood pressure. All of the above biological processes are involved in the pathogenesis of kidney diseases. Therefore, the activation of SIRT1 may become a therapeutic target to improve the clinical outcome of kidney diseases. In this review, we give an overview of SIRT1 and its molecular targets as well as SIRT1-modulated biological processes, with a particular focus on the role of SIRT1 in kidney diseases.

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“…14 Other groups have also described the use of formaldehyde fixation for claudin 1 18 and E-cadherin 19 immunofluorescence in breast cancer cells. In other cell models, formaldehyde or alcohol-based fixatives (including methanol) have alternatively been used for the immunofluorescence detection of claudin 1 18,20,21 or E-cadherin. 22,23 Interestingly, the direct comparison of different fixing protocols including formaldehyde and methanol fixation for the detection of claudin 1 in airway epithelial cells concluded that methanol fixation was optimal to obtain consistent and reliable signals.…”

Section: Discussionmentioning

confidence: 99%

Comparison of Fixation Methods for the Detection of Claudin 1 and E-Cadherin in Breast Cancer Cell Lines by Immunofluorescence

Edechi¹,

Amini²,

Hamedani³

et al. 2021

J Histochem Cytochem.

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The tight junction membrane protein claudin 1 and the adherens junction protein E-cadherin play critical roles in cell–cell communication and in cell signaling. As a result, their protein levels and distribution in cancer have been a focus of cancer researchers in recent years. The loss of sensitivity to contact inhibition and the establishment of invasive properties in cancer are thought to be a result of the mislocalization of these membrane proteins to the cytoplasm. However, reports on their distribution and levels have been inconsistent. It is therefore critical that the techniques used to determine the cellular localization of these proteins be both consistent and reliable. This study was undertaken to determine the optimal fixation method, methanol or formalin, for the detection of claudin 1 and E-cadherin by immunofluorescence in five different human breast cancer cell lines. Both methods exhibited staining of the cell membrane and cytoplasm, but the strongest and most distinct signals were obtained using methanol fixation. Interestingly, cell-specific differences were also observed that appeared to be associated with levels of claudin 1 and E-cadherin as seen by Western blotting. Therefore, when evaluating cellular localization of the junction proteins claudin 1 and E-cadherin, expression level and cell type differences must be considered:

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Expression of Tight Junction Protein Claudin-1 in Human Crescentic Glomerulonephritis

Cited by 10 publications

References 30 publications

Quantified Morphology of the Cervical and Subdiaphragmatic Vagus Nerves of Human, Pig, and Rat

Quantified Morphology of the Cervical and Subdiaphragmatic Vagus Nerves of Human, Pig, and Rat

Sirtuin 1: A Target for Kidney Diseases

Comparison of Fixation Methods for the Detection of Claudin 1 and E-Cadherin in Breast Cancer Cell Lines by Immunofluorescence

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