Expression of Thy-1 Antigen by Murine Epidermal Cells

Tschachler, Erwin; Schuler, Gerold; Hutterer, J.; Leibl, Heinz; Wolff, Klaus; Stingl, Georg

doi:10.1111/1523-1747.ep12518326

Cited by 229 publications

(88 citation statements)

References 18 publications

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“…It has been more than 20 years since two groups independently discovered Thy-1 + DETC within the murine epidermis (29,30), but the molecular mechanisms for DETC trafficking to the skin have been unclear. Here we show that DETC within the epidermis of adult mice express E-lig and P-lig, as judged by binding to E selectin-and P selectin-IgG chimeric molecules in a calcium-dependant fashion.…”

Section: Discussionmentioning

confidence: 99%

Embryonic trafficking of γδ T cells to skin is dependent on E/P selectin ligands and CCR4

Jiang

Campbell

Kupper

2010

Proc. Natl. Acad. Sci. U.S.A.

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Dendritic epidermal T cells (DETC) express an invariant Vγ3/Vδ1 T-cell receptor, appear in fetal epidermis, and form a population of resident epidermal T cells. Their temporal development in the thymus has been studied extensively. However, little is known about the mechanisms involved in the embryonic trafficking of DETC from thymus to epidermis. We demonstrate that DETC in adult skin, as well as the DETC precursors in fetal thymus, express E and P selectin ligands (E-and P-lig). Mice deficient in α1,3 fucosyltransferases IV and VII (FTIV/VII) cannot synthesize the carbohydrate motifs that form key elements of these selectin ligands. The numbers of DETC in the epidermis of FTIV/VII −/− mice were dramatically reduced compared with normal mice. However, the development of DETC precursors in fetal thymus was identical in normal and FTIV/VII −/− mice, suggesting that the DETC precursors produced in FTIV/VII −/− mice could not traffic effectively to skin because they lack E-and P-lig. We tested this hypothesis by daily injection of blocking antibodies against E and P selectin into pregnant mice. Mice born from dams treated with anti-selectin antibodies, but not those born from dams treated with isotype control, had significantly diminished numbers of DETC. To test the role of chemokine receptors in DETC skin homing, we examined skin from CCR4 −/− and CCR10 −/− mice, respectively. DETC were significantly reduced in CCR4 −/− mice but were present at normal levels in CCR10 −/− mice. Our results present evidence for the crucial role of trafficking molecules in embryonic migration of DETC precursors to skin. central to host defense against pathogens, and bear T-cell receptors (TCR) that are heterodimers of either α and β or γ and δ TCR genes. T cells with γδ TCR are much less abundant than αβ TCR cells in the adult thymus, blood, and peripheral lymphoid organs but are more abundant in epithelial layers of tissues, such as skin, intestine, lung, and genitourinary tract (1-4). Murine dendritic epidermal T cells (DETC) are a unique population of γδ T cells that reside in murine epidermis. They have a dendritic morphology in situ and express a distinctive invariant Vγ3/Vδ1 TCR that recognizes a putative self-antigen expressed on the damaged, stressed, or transformed keratinocytes (5). In the past decade, significant advances have been made in understanding the roles of DETC in tissue homeostasis and wound healing (6-8). Recently, DETC were shown to contribute to the regression of cutaneous tumors through NKG2D ligation and to regulate autoimmune skin disease (9, 10). DETC also may be important mediators in bridging innate and adaptive immunity of skin through communication with keratinocytes and Langerhans cells (11).The thymus is a unique site that provides an inductive environment for T-lineage commitment and V(D)J recombination at the γδ and αβ TCR loci. Vγ3/Vδ1 T cells are the first TCR-bearing cells to arise in the fetal thymus and selectively home to the skin between embryonic day (ED) 16 and 18 (12, 13). It initially...

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Section: Discussionmentioning

confidence: 99%

Embryonic trafficking of γδ T cells to skin is dependent on E/P selectin ligands and CCR4

Jiang

Campbell

Kupper

2010

Proc. Natl. Acad. Sci. U.S.A.

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“…16,22,31 They express the T-cell receptor (TCR) V-gamma 3ϩ/V-delta 1ϩ in association with the CD3 molecule, 19,20 but do not express the CD4 and CD8 molecules nor MHC class II antigens. 3,40,41 In contrast, Langerhans cells are of bone marrow origin and express MHC class II antigens but do not express the CD3 molecule. The functions of DETC are not well understood, but they appear to perform a surveillance role in the skin in addition to mediating effector functions through the production of immuneassociated soluble factors and a keratinocyte growth factor.…”

Section: Discussionmentioning

confidence: 99%

Cutaneous DNA Vaccination Against Ebola Virus by Particle Bombardment: Histopathology and Alteration of CD3-positive Dendritic Epidermal Cells

2001

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Abstract. We analyzed the localization of gold particles, expression of immunogenic protein, and histopathologic changes after vaccinating guinea pigs and mice with a DNA vaccine to the Ebola virus glycoprotein administered by cutaneous particle bombardment. Gold particles were deposited in all layers of the epidermis and in the dermis. Those in the epidermis were lost as the damaged layers sloughed, while those in the dermis were phagocytized by macrophages. Glycoprotein was demonstrated by immunohistochemistry primarily in keratinocytes in the epidermis and hair follicle epithelium and less frequently in dermal macrophages, fibroblasts, sebocytes, and cells that appeared to be Langerhans cells. The number of cells that expressed glycoprotein increased between 4 and 8 hours postvaccination, then decreased to near zero by 48 hours. The vaccine sites were histologically divisible into three zones. The central portion, zone 1, contained the most gold particles in the dermis and epidermis and had extensive tissue damage, including full-thickness epidermal necrosis. Zone 2 contained fewer gold particles in the epidermis and dermis and had less extensive necrosis. The majority of cells in which glycoprotein was expressed were in zone 2. Zone 3 contained gold particles only in the epidermis and had necrosis of only a few scattered cells. Regeneration of the epidermis in damaged areas was evident at 24 hours postvaccination and was essentially complete by day 5 in the mice and day 10 in the guinea pigs. Inflammatory changes were characterized by hemorrhage, edema, and infiltrates of neutrophils initially and by infiltrates of lymphocytes and macrophages at later times. In zone 1, inflammation affected both the epidermis and dermis. Peripherally, inflammation was relatively limited to the epidermis. CD3-positive dendritic epidermal cells were demonstrated in the epidermis and superficial hair follicles of unvaccinated immunocompetent mice and beige mice but not of SCID mice. These cells disappeared from all but the most peripheral portions of the vaccine sites of vaccinated mice within 24 hours. They reappeared slowly, failing to reach numbers comparable with unvaccinated mice by 35 days postvaccination. The epidermis of control guinea pigs also had CD3-positive cells, but they did not have dendrites. These findings should contribute to a better understanding of the mechanisms operating in response to DNA vaccination by particle bombardment.

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“…The epidermis of TCR(,Bx6) -/-mice contains a small number of Thy-lhi cells (data not shown), distinct from keratinocytes that can be Thy-110 (41). To determine whether Thy-lhi cells were responsible for TCRy/6 rearrangements detected in epidermal sheets of TCR(13xS) -/-mice, Thy-lhi cells were purified (Fig.…”

Section: %mentioning

confidence: 97%

Disruption of epithelial gamma delta T cell repertoires by mutation of the Syk tyrosine kinase.

Mallick-Wood

Pao

Cheng

et al. 1996

Proc. Natl. Acad. Sci. U.S.A.

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Chimeric mice in which lymphocytes are deficient in the Syk tyrosine kinase have been created. Compared with Syk-positive controls, mice with Syk -/-lymphocytes display substantial depletion of intraepithelial ya T cells in the skin and gut, with developmental arrest occurring after antigen receptor gene rearrangement. In this dependence on Syk, subsets of intraepithelial y8 T cells are similar to B Immunoblotting. Cells were lysed in 1% Nonidet P-40 lysis buffer with protease and phosphatase inhibitors. Post-nuclear supernatants were immunoprecipitated serially with anti-Syk and anti-Zap-70 antisera, followed by protein A Sepharose CL-4B beads (Pharmacia). After electrophoresis on SDS/9% polyacrylamide gels, proteins were transferred to poly(vinylidene difluoride) membrane (Bio-Rad), blotted with anti-Syk sera (1:100) or anti-Zap-70 sera (1:100), and detected by enhanced chemiluminescence (DuPont). Anti-Syk antiserum was against residues 260-370 (the unique region between the carboxyl-Src homology 2 domain and the kinase domain), and does not recognize

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Expression of Thy-1 Antigen by Murine Epidermal Cells

Cited by 229 publications

References 18 publications

Embryonic trafficking of γδ T cells to skin is dependent on E/P selectin ligands and CCR4

Embryonic trafficking of γδ T cells to skin is dependent on E/P selectin ligands and CCR4

Cutaneous DNA Vaccination Against Ebola Virus by Particle Bombardment: Histopathology and Alteration of CD3-positive Dendritic Epidermal Cells

Disruption of epithelial gamma delta T cell repertoires by mutation of the Syk tyrosine kinase.

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