Expression of three gonadotropin subunits in Southern catfish gonad and their possible roles during early gonadal development

Wu, Fengrui; Zhang, Xiuyue; Zhang, Weili; Huang, Biao; Liu, Zhihao; Hu, Chongjiang; Wang, Deshou

doi:10.1016/j.cbpa.2008.12.013

Cited by 28 publications

(17 citation statements)

References 13 publications

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“…In addition to the pituitary, the fish testis, but not the ovary, expressed fshb mRNA at a detectable level. fshb mRNA is expressed in the ovaries of Southern catfish [29], gilthead seabream [30], zebrafish [31], and marbled eel [32]. In the zebrafish, fshb mRNA is also detectable in the testis [31].…”

Section: Discussionmentioning

confidence: 99%

Characterization of Luteinizing Hormone and Luteinizing Hormone Receptor and Their Indispensable Role in the Ovulatory Process of the Medaka

et al. 2013

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The molecular properties and roles of luteinizing hormone (Lh) and its receptor (Lhcgrbb) have not been studied for the medaka (Oryzias latipes), which is an excellent animal model for ovulation studies. Here, we characterized the medaka Lh/Lhcgrbb system, with attention to its involvement in the ovulatory process of this teleost fish. In the medaka ovary, follicle-stimulating hormone receptor mRNA was expressed in small and medium-sized follicles, while lhcgrbb mRNA was expressed in the follicle layers of all growing follicles. Experiments using HEK 293T cells expressing medaka Lhcgrbb in vitro revealed that gonadotropin from pregnant mare’s serum and medaka recombinant Lh (rLh) bound to the fish Lhcgrbb. The fish gonadotropin subunits Gtha, Fshb, and Lhb were essentially expressed at fairly constant levels in the pituitary of the fish during a 24-h spawning cycle. Using medaka rLh, we developed a follicle culture system that allowed us to follow the whole process of oocyte maturation and ovulation in vitro. This follicle culture method enabled us to determine that the Lh surge for the preovulatory follicle occurred in vivo between 19 and 15 h before ovulation. The present study also showed that oocyte maturation and ovulation were delayed several hours in vitro compared with in vivo. Treatment of large follicles with medaka rLh in vitro significantly increased the expression of Mmp15, which was previously demonstrated to be crucial for ovulation in the fish. These findings demonstrate that Lh/Lhcgrbb is critically involved in the induction of oocyte maturation and ovulation.

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Section: Discussionmentioning

confidence: 99%

Characterization of Luteinizing Hormone and Luteinizing Hormone Receptor and Their Indispensable Role in the Ovulatory Process of the Medaka

et al. 2013

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“…Although many studies focused on temperature-induced expression changes of sex differentiation genes in the gonad, little is known regarding brain gene expression changes induced by high temperature in fish. In Southern catfish, GTHα were expressed in the pituitary, higher in female than in male (Wu et al, 2009). In the rainbow trout, GTHα expression increased throughout gametogenesis and periovulatory period (Gomez et al, 1999).…”

Section: Tablementioning

confidence: 97%

“…The expression of DMRT1 in the gonad was significantly correlated with CYP19A1B and ERα in the female brain, and correlated with ERα and ERβ in the male brain. Previous reports showed that GTH, FSH, and LH play important roles in gonadal development and are the most likely candidates to elicit early gonadal differentiation in vertebrates including fish (Wu et al, 2009). The androgen receptor is a ligand-dependent transcription factor and androgens generally act on target cells through ARs (Tosaka et al, 2010).…”

Section: Tablementioning

confidence: 99%

Differential expression analysis of genes involved in high-temperature induced sex differentiation in Nile tilapia

Wang

Chen

et al. 2014

Comparative Biochemistry and Physiology Part B: Biochemistry an

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“…Fourteen genes were down-regulated, with the follicle stimulating hormone beta subunit included. The follicle stimulating hormone is secreted by pituitary and showed great importance in early gonad differentiation and follicle development [40–42]. Twenty-five genes were differentially expressed between I_brain and M_brain, including 18 up-regulated genes and 7 down-regulated ones (S3 Table).…”

Section: Resultsmentioning

confidence: 99%

Genome-wide analysis of brain and gonad transcripts reveals changes of key sex reversal-related genes expression and signaling pathways in three stages of Monopterus albus

Chi

Gao

et al. 2017

PLoS ONE

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BackgroundThe natural sex reversal severely affects the sex ratio and thus decreases the productivity of the rice field eel (Monopterus albus). How to understand and manipulate this process is one of the major issues for the rice field eel stocking. So far the genomics and transcriptomics data available for this species are still scarce. Here we provide a comprehensive study of transcriptomes of brain and gonad tissue in three sex stages (female, intersex and male) from the rice field eel to investigate changes in transcriptional level during the sex reversal process.ResultsApproximately 195 thousand unigenes were generated and over 44.4 thousand were functionally annotated. Comparative study between stages provided multiple differentially expressed genes in brain and gonad tissue. Overall 4668 genes were found to be of unequal abundance between gonad tissues, far more than that of the brain tissues (59 genes). These genes were enriched in several different signaling pathways. A number of 231 genes were found with different levels in gonad in each stage, with several reproduction-related genes included. A total of 19 candidate genes that could be most related to sex reversal were screened out, part of these genes’ expression patterns were validated by RT-qPCR. The expression of spef2, maats1, spag6 and dmc1 were abundant in testis, but was barely detected in females, while the 17β-hsd12, zpsbp3, gal3 and foxn5 were only expressed in ovary.ConclusionThis study investigated the complexity of brain and gonad transcriptomes in three sex stages of the rice field eel. Integrated analysis of different gene expression and changes in signaling pathways, such as PI3K-Akt pathway, provided crucial data for further study of sex transformation mechanisms.

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Expression of three gonadotropin subunits in Southern catfish gonad and their possible roles during early gonadal development

Cited by 28 publications

References 13 publications

Characterization of Luteinizing Hormone and Luteinizing Hormone Receptor and Their Indispensable Role in the Ovulatory Process of the Medaka

Characterization of Luteinizing Hormone and Luteinizing Hormone Receptor and Their Indispensable Role in the Ovulatory Process of the Medaka

Differential expression analysis of genes involved in high-temperature induced sex differentiation in Nile tilapia

Genome-wide analysis of brain and gonad transcripts reveals changes of key sex reversal-related genes expression and signaling pathways in three stages of Monopterus albus

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