Expression of thimet oligopeptidase (THOP) modulated by oxidative stress in human multidrug resistant (MDR) leukemia cells

Neves, Raquel Leão; Marem, Alyne; Carmona, Bruno; Arata, Júlia Galanakis; Ramos, Marcos Paulo Cyrillo; Justo, Giselle Z.; Melo, Fabiana Henriques Machado de; Oliveira, Vitor; Icimoto, Marcelo Yudi

doi:10.1016/j.biochi.2023.03.013

Cited by 2 publications

(1 citation statement)

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“…Despite the biochemical similarities of Nln and THOP1 among most substrates, neurotensin (pyr-Glu-Leu-Tyr-Glu-Asn-Lys-Pro-Arg-Arg-Pro-Tyr-Ile-Leu-OH) is cleaved by Nln at the Pro 10 -Tyr 11 bond, while THOP1 cleaves the Arg 8 -Arg 9 bond [1]. Moreover, Nln and THOP1 can also be distinguished by their sensitivity to carboalkyls, phosphinic and Pro-Ile inhibitors [23,33,34], and their drastic, distinct sensitivity to thiol compounds [35][36][37][38].…”

Section: Introductionmentioning

confidence: 99%

Neurolysin Knockout Mice in a Diet-Induced Obesity Model

Caprioli,

Eichler,

Silva

et al. 2023

IJMS

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Neurolysin oligopeptidase (E.C.3.4.24.16; Nln), a member of the zinc metallopeptidase M3 family, was first identified in rat brain synaptic membranes hydrolyzing neurotensin at the Pro-Tyr peptide bond. The previous development of C57BL6/N mice with suppression of Nln gene expression (Nln-/-), demonstrated the biological relevance of this oligopeptidase for insulin signaling and glucose uptake. Here, several metabolic parameters were investigated in Nln-/- and wild-type C57BL6/N animals (WT; n = 5–8), male and female, fed either a standard (SD) or a hypercaloric diet (HD), for seven weeks. Higher food intake and body mass gain was observed for Nln-/- animals fed HD, compared to both male and female WT control animals fed HD. Leptin gene expression was higher in Nln-/- male and female animals fed HD, compared to WT controls. Both WT and Nln-/- females fed HD showed similar gene expression increase of dipeptidyl peptidase 4 (DPP4), a peptidase related to glucagon-like peptide-1 (GLP-1) metabolism. The present data suggest that Nln participates in the physiological mechanisms related to diet-induced obesity. Further studies will be necessary to better understand the molecular mechanism responsible for the higher body mass gain observed in Nln-/- animals fed HD.

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Section: Introductionmentioning

confidence: 99%

Neurolysin Knockout Mice in a Diet-Induced Obesity Model

Caprioli,

Eichler,

Silva

et al. 2023

IJMS

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Effect of chiral silver nanoparticles on prolyl-oligopeptidase binding and activity

Batista,

Toledo,

Ramos

et al. 2024

Front. Nanotechnol.

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Introduction: Silver nanoparticles have a diversity of applications both in biological and technological areas. More recently, studies conducted in the Nano/Bio interface have demonstrated that chiral nanocrystals grew in chiral templates, and nanostructures functionalized with chiral molecules present specific properties. These properties apply to advanced materials, energy, medicine, and pharmacology.Methodology: The present study synthesized silver nanoparticles on silver seeds using D- and L-histidine as templates and borohydride as a reducing agent.Results and Discussion: The nanoparticles were characterized by UV-visible spectroscopy and presented surface plasmon resonance (SPR) bands around 415 nm. CD spectra showed signals in the region of the SPR band, indicating the growth of nanocrystals with chiral distortion. Synthesized silver nanoparticles were also characterized by high-resolution transmission electron microscopy (HRTEM), which evidenced the presence of histidine corona. The silver nanoparticles were functionalized with prolyl-oligopeptidase (POP), a prolinespecific endopeptidase expressed in the brain. This enzyme cleaves neuroactive peptides involved in memory, learning, and neurodegeneration. The enzyme POP was expressed with a His-tag to provide competitive binding affinity to silver nanoparticles covered by D- and L-histidine. Considering the biological importance, POP was chosen as a model for studying the functionalization of chiral silver nanoparticles regarding the chiral discrimination for binding affinity and stabilization.

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Expression of thimet oligopeptidase (THOP) modulated by oxidative stress in human multidrug resistant (MDR) leukemia cells

Cited by 2 publications

References 68 publications

Neurolysin Knockout Mice in a Diet-Induced Obesity Model

Neurolysin Knockout Mice in a Diet-Induced Obesity Model

Effect of chiral silver nanoparticles on prolyl-oligopeptidase binding and activity

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