Expression of the Vitreoscilla Hemoglobin (VHb)-Encoding Gene in Transgenic White Poplar: Plant Growth and Biomass Production, Biochemical Characterization and Cell Survival under Submergence, Oxidative and Nitrosative Stress Conditions

Zelasco, Samanta; Reggi, Serena; Calligari, Paolo; Balestrazzi, Alma; Bongiorni, Cristina; Quattrini, E.; Delia, G.; Bisoffi, Stefano; Fogher, Corrado; Confalonieri, Massimo

doi:10.1007/s11032-005-5295-3

Cited by 26 publications

(18 citation statements)

References 31 publications

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“…Similar results regarding increased germination and submergence tolerance were seen in Arabidopsis thaliana engineered to express vgb ); the Arabidopsis transgenics also gained increased tolerance to both oxidative and nitrosative stress. Zelasco et al (2006) observed generally improved growth, but not improved submergence tolerance (see below), of transgenic white poplar coincident with VHb expression.…”

Section: Enhancement Of Growth Of Higher Plantsmentioning

confidence: 77%

Recent advances in understanding the structure, function, and biotechnological usefulness of the hemoglobin from the bacterium Vitreoscilla

2011

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The hemoglobin from the bacterium Vitreoscilla (VHb) is the first microbial hemoglobin that was conclusively identified as such (in 1986). It has been extensively studied with respect to its ligand binding properties and mechanisms, structure, biochemical functions, and the mechanisms by which its expression is controlled. In addition, cloning of its gene (vgb) into a variety of heterologous hosts has proved that its expression results substantial increases in production of a variety of useful products and ability to degrade potentially harmful compounds. Recent studies (since 2005) have added significant knowledge to all of these areas and shown the broad range of biotechnological applications in which VHb can have a positive effect.

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Section: Enhancement Of Growth Of Higher Plantsmentioning

confidence: 77%

Recent advances in understanding the structure, function, and biotechnological usefulness of the hemoglobin from the bacterium Vitreoscilla

2011

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“…At that time all the total culturable bacteria isolated from the soil were able to produce the expected hydrolysis halo on DMG medium. It is worth noting that the trial with the white poplars was carried out under greenhouse conditions which were chosen to resemble the field cultivation (Zelasco et al, 2006a). The soil temperature in the greenhouse was constantly monitored and the highest values were observed in August 2004 (data not shown).…”

Section: Discussionmentioning

confidence: 99%

“…'Villafranca') tolerant to the herbicide phosphinothricin (PPT), a specific inhibitor of glutamine synthetase in plants and microorganisms (Confalonieri et al, 2000). Among woody species, white poplar and particularly the elite cultivar 'Villafranca', is considered a model system in tree biotechnology for the production of GM plants expressing relevant traits (Giorcelli et al, 2004;Balestrazzi et al, 2006;Zelasco et al, 2006a).…”

Section: Introductionmentioning

confidence: 99%

Nuclease-producing bacteria in soil cultivated with herbicide resistant transgenic white poplars

2007

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This study was carried out using soil cultivated, under greenhouse conditions, with transgenic white poplars expressing the bar gene for tolerance to the Basta ® herbicide. The occurrence of extracellular nucleolytic activity was monitored in soil samples collected at four different times over a 26-month period. The fraction of nuclease producing bacteria (NPB) ranged from 62.5 to 100% of the total culturable bacterial population. The DNA-methyl green plate assay allowed to distinguish five groups of bacteria showing increasing levels of extracellular DNase activity. The NPB isolates were classified by 16S rDNA sequence analysis as members of the Bacillus, Brevibacillus, Microbacterium, Pseudomonas and Stenotrophomonas genera. For each genus, NPB isolates were cultured in liquid medium and the nucleolytic activity during different growth phases was monitored. Production of extracellular nucleases was observed only during the mid-exponential growth phase of the Brevibacillus, Microbacterium and Stenotrophomonas isolates, while no activity was evidenced for isolates classified within the Bacillus and Pseudomonas genera.

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“…From this point of view, the white poplar (Populus alba L.) cultivar 'Villafranca' used in this work represents an ideal system, due to the high biomass production and resprouting ability (Confalonieri et al, 2000). In recent years, the same cultivar has been modified by gene transfer in order to acquire several agronomically relevant traits (Giorcelli et al, 2004;Zelasco et al, 2006;Balestrazzi et al, 2006), tested with innovative marker-free gene-transfer technologies (Zelasco et al, 2007) and recently utilized for phytoremediation purposes (Castiglione et al, 2007;Lingua et al, 2008).…”

Section: Introductionmentioning

confidence: 99%

Occurrence of multiple metal-resistance in bacterial isolates associated with transgenic white poplars (Populus alba L.)

et al. 2009

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The occurrence of multiple metal-resistance was assessed in two bacterial collections, named Herbicide Resistant Bacteria (HRB) and Nuclease-Producing Bacteria (NPB) respectively, consisting of 15 and 11 isolates obtained from a loamy sand cultivated with transgenic white poplars (Populus alba L., cv 'Villafranca') engineered for herbicide resistance. A third collection of 11 bacterial isolates, named Leaf-Associated Bacteria (LAB), obtained from the leaves of transgenic white poplars expressing the StSy gene for resveratrol production and from untransformed plants was evaluated. Resistance to Cd, Co, Cu, Pb and Zn was tested. As for the HRB collection, nine different phenotypes were monitored, which included tetra-, tri-and double-resistance. Tri-and double-metal resistance occurred also within the NPB and LAB collections. In both cases five different phenotypes were recovered. An additional investigation was carried out on the HRB-1c isolate, resistant to Cd, Co, Pb and Zn, which was previously demonstrated to produce indoleacetic acid, a plantgrowth-promoting trait. Colorimetric assays, performed on the cell-depleted medium of HRB-1c liquid cultures grown in presence of heavy metals, confirmed that this trait was not affected. A 19-kb plasmid, possibly involved in the maintenance of the multiple metalresistant phenotype, was detected in the HRB-1c cells.

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Expression of the Vitreoscilla Hemoglobin (VHb)-Encoding Gene in Transgenic White Poplar: Plant Growth and Biomass Production, Biochemical Characterization and Cell Survival under Submergence, Oxidative and Nitrosative Stress Conditions

Cited by 26 publications

References 31 publications

Recent advances in understanding the structure, function, and biotechnological usefulness of the hemoglobin from the bacterium Vitreoscilla

Recent advances in understanding the structure, function, and biotechnological usefulness of the hemoglobin from the bacterium Vitreoscilla

Nuclease-producing bacteria in soil cultivated with herbicide resistant transgenic white poplars

Occurrence of multiple metal-resistance in bacterial isolates associated with transgenic white poplars (Populus alba L.)

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