Expression of the vesicular stomatitis virus nucleocapsid protein controlled by the lambda PR and PL promoters in Escherichia coli

Expression of cloned monkey metallothionein in Escherichia coli

“…Previously, we succeeded in expressing a cDNA specifying the vesicular stomatitis virus nucleocapsid protein from the A PR promoter in E. coli K-12AHlAtrp (c1857 ) by heat induction at 42°C. Expression of the protein was strongly repressed at 28°C (11). We therefore used a similar system to express the MT protein.…”

“…We therefore used a similar system to express the MT protein. We started the construction with the pCR3 vector (11). This plasmid carries a thermolabile repressor, c1857, the A PR promoter, a ribo-* Corresponding author.…”

“…The poor expression of MT-TI might be due to the poor translational efficiency of the mRNA encoding MT, since foreign genes were efficiently transcribed by the same expression vector (11,12). In the expression of the methionyl bovine growth hormone in E. coli, the poor translational efficiency of the native gene transcripts is improved by construction of a two-cistron system (14).…”