Expression of the TRPM8-immunoreactivity in dorsal root ganglion neurons innervating the rat urinary bladder

Hayashi, Tokumasa; Kondo, Teruyoshi; Ishimatsu, Masaru; Yamada, Satoko; Nakamura, Kei‐ichiro; Matsuoka, Kei; Akasu, Takashi

doi:10.1016/j.neures.2009.07.005

Cited by 50 publications

(50 citation statements)

References 43 publications

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“…Up to fifty percent of TRPM8-expressing sensory neurons also express TRPV1 both under normal conditions and after injury [3,[56][57][58][59]. Some TRPM8 neurons express other markers of a nociceptive phenotype including CGRP and Substance P while others do not, suggesting a large percentage of these cells are non-peptidergic afferents [56][57][58][60][61][62]. However these remaining cells do not bind the nonpeptidergic marker IB 4 , the classical marker of this cell-type, thereby suggesting that TRPM8 may label an as yet unappreciated population of non-peptidergic, non-IB 4 binding neurons [56].…”

Section: Neurochemical Phenotypementioning

confidence: 99%

TRPM8: From Cold to Cancer, Peppermint to Pain

Knowlton

McKemy²

2011

CPB

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Temperature perception is vital for cellular and metabolic homeostasis, avoidance, and survival. In the primary afferent nerve terminal, select members of the transient receptor potential (TRP) family of ion channels reside and convert thermal stimuli into neuronal activity. The cold and menthol receptor, TRPM8, is the predominant thermoceptor for cellular and behavioral responses to cold temperatures. Remarkably, this single molecular sensor of cold, that responds at a discrete thermal threshold in vitro (approximately 28°C), enables sensory afferents to respond to distinct, yet varied thermal thresholds (approximately 28 to <5°C). Thus, unlike other thermally-gated TRP channels which are activated at either innocuous or noxious temperatures, TRPM8 provides perception of both pleasantly cool and painfully cold. In addition to this diversity in sensory signaling, TRPM8 has an emerging role in a variety of biological systems, including thermoregulation, cancer, bladder function, and asthma. Here we summarize some key points related to TRPM8 and its potential as a drug target to treat a wide variety of physiological conditions. Nonetheless, it remains to be seen how this single "cool" molecule can serve in such a multitude of biological processes.

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Section: Neurochemical Phenotypementioning

confidence: 99%

TRPM8: From Cold to Cancer, Peppermint to Pain

Knowlton

McKemy²

2011

CPB

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“…The cold sensing receptor TRPM8 has been implicated in the contractile response to a cooling stimulus (known as the bladder cooling reflex) used in the diagnosis of bladder disorders such as OABS. Expression of TRPM8 has been identified on bladder afferent fibres and on bladder innervating DRG neurons in which it colocalizes with nociceptive markers such as cGRP and IB4 [42]. Previously, Lashinger et al [43] showed that application of a TRPM8 channel blocker decreased voiding frequency and abdominal motor responses in the rat suggesting that in addition to cold sensing, TRPM8 may also be involved in the afferent control of micturition and nociception.…”

Section: Transient Receptor Potential Cation Channelsmentioning

confidence: 99%

The afferent system and its role in lower urinary tract dysfunction

Daly

Collins

Chapple

et al. 2011

Current Opinion in Urology

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Recent studies provide further evidence that afferent control of the bladder may be dependent on integration of excitatory and inhibitory mediators from the urothelium such as ATP and nitric oxide. A number of studies have examined the role cholinergic and adrenergic mechanisms play in bladder afferent function, and several new potential mechanisms involving the cannabinoid receptors and transient receptor potential channels have emerged as areas which warrant further investigation. A better understanding of afferent mechanisms in the bladder will hopefully lead to more effective treatments of lower urinary tract disorders.

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“…Using retrograde labelling, we have shown that L6 and S1 dorsal root ganglia contain sensory neurones that innervate the guinea-pig bladder and demonstrated the presence of TRPM8 immunoreactivity in some of these traced neurones. TRPM8 mRNA and protein have been detected previously in rat and human urothelium (Du et al, 2008;Stein et al, 2004;Mukerji et al 2006b), and TRPM8 protein in a small proportion of rat L6 and S1 dorsal root ganglia neurones retrogradely labelled from the bladder (Hayashi et al, 2009). However, species differences may exist in the location of TRPM8 in the bladder as neither TRPM8 mRNA nor protein were detected in rat or human bladder smooth muscle (Du et al, 2008;Stein et al, 2004).…”

Section: Discussionmentioning

confidence: 93%

“…In the cat, normal micturition has been shown to involve activation of myelinated (A-δ) mechanoreceptor afferents (Habler et al, 1993) and a spinal-ponto-spinal reflex pathway (Holstege et al, 1986). Both C-and A-δ-mechanoreceptor afferents may be involved in normal micturition in the rat (Shea et al, 2000) and TRPM8-immunoreactivity has been detected in both types of dorsal root ganglia neurones innervating the rat bladder (Hayashi et al, 2009). The relative contribution of these different fibre types to normal micturition in the guinea-pig is unknown and involvement of neuronal and/or urothelial TRPM8 uncertain.…”

Section: Discussionmentioning

confidence: 99%