Expression of the sub-pathways of the Chloroflexus aurantiacus 3-hydroxypropionate carbon fixation bicycle in E. coli: Toward horizontal transfer of autotrophic growth

Mattozzi, Matthew D.; Ziesack, Marika; Voges, Mathias J.; Silver, Pamela A.; Way, Jeffrey C.

doi:10.1016/j.ymben.2013.01.005

Cited by 81 publications

(54 citation statements)

References 63 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Rubisco is not the only carboxylase in nature, and there are at least five known pathways of carbohydrate synthesis from CO 2 that use other carboxylases (Fuchs, 2011). However, only one of these, the 3-hydroxypropionate (3-HPA) bicycle, appears oxygen insensitive (Mattozzi et al, 2013). These existing pathways or totally synthetic pathways could be introduced into higher plants ( Bar-Even et al, 2010).…”

Section: Targets For Increasing Crop Photosynthetic Efficiencymentioning

confidence: 99%

Meeting the Global Food Demand of the Future by Engineering Crop Photosynthesis and Yield Potential

Long

Marshall‐Colón

Zhu

2015

Cell

837

580

View full text Add to dashboard Cite

Increase in demand for our primary foodstuffs is outstripping increase in yields, an expanding gap that indicates large potential food shortages by mid-century. This comes at a time when yield improvements are slowing or stagnating as the approaches of the Green Revolution reach their biological limits. Photosynthesis, which has been improved little in crops and falls far short of its biological limit, emerges as the key remaining route to increase the genetic yield potential of our major crops. Thus, there is a timely need to accelerate our understanding of the photosynthetic process in crops to allow informed and guided improvements via in-silico-assisted genetic engineering. Potential and emerging approaches to improving crop photosynthetic efficiency are discussed, and the new tools needed to realize these changes are presented.

show abstract

Section: Targets For Increasing Crop Photosynthetic Efficiencymentioning

confidence: 99%

Meeting the Global Food Demand of the Future by Engineering Crop Photosynthesis and Yield Potential

Long

Marshall‐Colón

Zhu

2015

Cell

837

580

View full text Add to dashboard Cite

show abstract

“…Because the shared initial steps of the pathways enable acetyl-CoA to be converted to propionyl-CoA, it could be possible to source propionate from acetyl-CoA resulting from glucose dissimilation (Figure 1(CII)). Indeed, the 3HP cycle has been reconstituted successfully in E. coli in sub-pathways including that for the production of propionyl-CoA [56], but to date there are no reports in the literature attempting to utilise this pathway for the production of propionic acid. A key limitation of this strategy is the high ATP requirements of these reactions; consuming a net two ATP and two NADPH per acetyl-CoA consumed, resulting in a maximum theoretical yield of 1.33 mol propionate/mol glucose with no ATP generation (Table 5).…”

Section: Hp/4hb Cyclesmentioning

confidence: 99%

Microbial Propionic Acid Production

et al. 2017

View full text Add to dashboard Cite

Propionic acid (propionate) is a commercially valuable carboxylic acid produced through microbial fermentation. Propionic acid is mainly used in the food industry but has recently found applications in the cosmetic, plastics and pharmaceutical industries. Propionate can be produced via various metabolic pathways, which can be classified into three major groups: fermentative pathways, biosynthetic pathways, and amino acid catabolic pathways. The current review provides an in-depth description of the major metabolic routes for propionate production from an energy optimization perspective. Biological propionate production is limited by high downstream purification costs which can be addressed if the target yield, productivity and titre can be achieved. Genome shuffling combined with high throughput omics and metabolic engineering is providing new opportunities, and biological propionate production is likely to enter the market in the not so distant future. In order to realise the full potential of metabolic engineering and heterologous expression, however, a greater understanding of metabolic capabilities of the native producers, the fittest producers, is required.

show abstract

“…So far, attempts have been initiated to introduce carbon fixation pathways, and in one study all genes for enzymes for the 3-hydroxypropionate bicycle have been expressed (Mattozzi et al, 2013); however, to our knowledge, no autotrophic pathway has yet been successfully introduced that sustains biomass formation. Additionally, a synthetic CO 2 -fixing photorespiratory bypass has been implemented recently (Shih et al, 2014).…”

Section: Introductionmentioning

confidence: 99%