Expression of the secreted FAD-dependent sulfydryl oxidase (QSOX) in the guinea pig central nervous system

Amiot, C.; Musard, Jean-François; Hadjiyiassemis, M.; Jouvenot, Michèle; Fellmann, D; Risold, Pierre‐Yves; Adami, Pascale

doi:10.1016/j.molbrainres.2004.02.024

Cited by 17 publications

(12 citation statements)

References 14 publications

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“…The labeling appearance is more probably related to the intracellular localization of rQSOX and its function in a given type of neuron and at a given moment. Which cell compartment(s) is (are) labeled is not clear; the different aspects observed (polarized or reticular) are compatible with a localization of rQSOX in the Golgi apparatus and in secretory granules, as shown in adult rat magnocellular neurons of the supraoptic nucleus (Mairet-Coello et al, 2004), suggesting this protein, which possesses a signal peptide, may follow the secretory pathway and probably plays a role extracellularly, as previously proposed (Coppock et al, 1993;Hoober and Thorpe 1999;Hoober et al, 1999a,b;Benayoun et al, 2001;Thorpe et al, 2002;Amiot et al, 2004). However, an endoplasmic reticulum or plasma membrane localization, as observed for other members of the QSOX family, is not impossible (Thorpe et al, 2002;Wittke et al, 2003).…”

Section: Features Of the Rqsox Immunolabeling In Maturing Neuronssupporting

confidence: 53%

“…A major role of QSOX family members is to introduce disulfide bonds in proteins. In mature brain, rQSOX might support functions such as correct folding of secreted neuropeptides and synaptic strengthening (Mairet-Coello et al, 2004;Amiot et al, 2004). The occurrence of rQSOX in fetal brain neurons suggests a role for this enzyme during brain development.…”

Section: Discussionmentioning

confidence: 99%

“…Nonetheless, the onset of rQSOX rise in the magnocellular cells of the hypothalamus coincides with the establishment of synaptic inputs (Khatchaturian and Sladek, 1980) and relationships of the outgrowing neurosecretory fibers with the pituicytes (Krisch, 1980). rQSOX might well intervene in the remodeling of extracellular matrix components and synaptic strengthening, as suggested before (Thorpe et al, 2002;Mairet-Coello et al, 2004;Amiot et al, 2004).…”

mentioning

confidence: 87%

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Ontogenesis of the sulfhydryl oxidase QSOX expression in rat brain

Mairet-Coello

Tury

Fellmann

et al. 2005

J of Comparative Neurology

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The spatiotemporal pattern of distribution of the sulfhydryl oxidase QSOX throughout ontogeny was mapped in rat brain using immunohistochemistry. The enzyme was detected on embryonic day (E) 12 in the dawning mantle layer, but the adult-like pattern was acquired postnatally around day 30 (P30). Throughout ontogenesis, rQSOX was detected in immature and mature neurons, but not in glial cells. The rQSOX developmental pattern can be divided into four periods: on E12 the enzyme was detected in the brainstem, more precisely in motoneurons; later (E16), rQSOX-positive cells were also observed in the forebrain, in the caudoputamen, and the subventricular zone. During late embryogenesis (E18-20), the amount of rQSOX cells considerably increased throughout the brain; they initially appeared in the hippocampus, then in the isocortex. From birth onwards, complex modifications of the rQSOX distribution occurred leading to the adult pattern by P30. Although rQSOX exhibits an overall increasing spatiotemporal pattern of distribution, different expression strategies were distinguished depending on the cell type or brain area. By comparing the rQSOX ontogeny with data on neurogenesis and brain histogenesis, we hypothesize that the enzyme could play a role in guiding migrating cells, their settling, and neuronal maturation, e.g., during outgrowth and synaptogenesis.

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Section: Features Of the Rqsox Immunolabeling In Maturing Neuronssupporting

confidence: 53%

Section: Discussionmentioning

confidence: 99%

mentioning

confidence: 87%

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Ontogenesis of the sulfhydryl oxidase QSOX expression in rat brain

Mairet-Coello

Tury

Fellmann

et al. 2005

J of Comparative Neurology

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“…QSOX distribution has already been intensively investigated in other environments, such as rat ) and guinea pig (Amiot et al 2004) central nervous system, rat peripheral tissues (Tury et al 2006) Fig. 2 Representative immunohistochemical localization of QSOX in E 13.5 mouse fetal tissues.…”

Section: Discussionmentioning

confidence: 99%

“…In this scenario, while short QSOX has been reported to cooperate with Pdi (Hoober et al 1999b;Thorpe and Coppock 2007), the long isoform was shown to be not as efficient as Ero-1-p to reoxidize Pdi (Chakravarthi et al 2007). In addition, QSOX has been proposed to be involved in extracellular matrix remodeling (Coppock et al 1998;Thorpe and Coppock 2007), since it is secreted (Chang and Zirkin 1978;Hoober et al 1996, Coppock et al 2000Matsuba et al 2002;Amiot et al 2004;Thorpe and Coppock 2007) and extracellular matrix proteins are rich in disulfide bonds. Additional participation in cell cycle regulation has also been considered, given that QSOX expression is up-regulated in G0-phase (Coppock 2000;Musard et al 2001).…”

Section: Introductionmentioning

confidence: 99%

Tissue distribution of quiescin Q6/sulfhydryl oxidase (QSOX) in developing mouse

et al. 2007

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Quiescin Q6/sulfhydryl oxidases (QSOX) are revisited thiol oxidases considered to be involved in the oxidative protein folding, cell cycle control and extracellular matrix remodeling. They contain thioredoxin domains and introduce disulfide bonds into proteins and peptides, with the concomitant hydrogen peroxide formation, likely altering the redox environment. Since it is known that several developmental processes are regulated by the redox state, here we assessed if QSOX could have a role during mouse fetal development. For this purpose, an anti-recombinant mouse QSOX antibody was produced and characterized. In E(13.5), E(16.5) fetal tissues, QSOX immunostaining was confined to mesoderm- and ectoderm-derived tissues, while in P1 neonatal tissues it was slightly extended to some endoderm-derived tissues. QSOX expression, particularly by epithelial tissues, seemed to be developmentally-regulated, increasing with tissue maturation. QSOX was observed in loose connective tissues in all stages analyzed, intra and possibly extracellularly, in agreement with its putative role in oxidative folding and extracellular matrix remodeling. In conclusion, QSOX is expressed in several tissues during mouse development, but preferentially in those derived from mesoderm and ectoderm, suggesting it could be of relevance during developmental processes.

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Sulfhydryl oxidases: sources, properties, production and applications

Faccio

Nivala

Kruus

et al. 2011

Appl Microbiol Biotechnol

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The formation of disulfide bonds in proteins and small molecules can greatly affect their functionality. Sulfhydryl oxidases (SOXs) are enzymes capable of oxidising the free sulfhydryl groups in proteins and thiol-containing small molecules by using molecular oxygen as an electron acceptor. SOXs have been isolated from the intracellular compartments of many organisms, but also secreted SOXs are known. These latter enzymes are generally active on small compounds and their physiological role is unknown, whereas the intracellular enzymes prefer proteins as substrates and are involved in protein folding. An increasing number of scientific publications and patent applications on SOXs have been published in recent years. The present mini-review provides an up-to-date summary of SOXs from various families, their production and their actual or suggested applications. The sequence features and domain organisation of the characterised SOXs are reviewed, and special attention is paid to the physicochemical features of the enzymes. A review of patents and patent applications regarding this class of enzymes is also provided.

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Expression of the secreted FAD-dependent sulfydryl oxidase (QSOX) in the guinea pig central nervous system

Cited by 17 publications

References 14 publications

Ontogenesis of the sulfhydryl oxidase QSOX expression in rat brain

Ontogenesis of the sulfhydryl oxidase QSOX expression in rat brain

Tissue distribution of quiescin Q6/sulfhydryl oxidase (QSOX) in developing mouse

Sulfhydryl oxidases: sources, properties, production and applications

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