Expression of the Respiratory Syncytial Virus 22K Protein on the Surface of Infected HeLa Cells

Routledge, E. G.; Willcocks, M. M.; Morgan, L.; Samson, A. C. R.; Scott, R.; Toms, G. L.

doi:10.1099/0022-1317-68-4-1217

Cited by 18 publications

(10 citation statements)

References 12 publications

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“…Unlike the F and G glycoproteins M2 was not removed from virions of RS virus simply by treatment with the non-ionic detergent Triton X-100 in low salt (Huang et al, 1985). Most cell-associated M2 was not exposed at the infected cell surface (Routledge et al, 1985(Routledge et al, , 1987 and fluorescent antibody studies have indicated that M2 might be associated with the same internal structures of RS virus as the nucleocapsid and phosphoprotein proteins and be in a location different to that of the M protein (Routledge et al, 1987). As with RS virus, the hydropathicity plot of the M2 of TRTV revealed that there were no extensive highly hydrophobic sequences of the sort present in proteins which are integrally associated with membranes (data not shown).…”

Section: Discussionmentioning

confidence: 97%

Sequence and in vitro expression of the M2 gene of turkey rhinotracheitis pneumovirus

Davis

Brown

et al. 1992

Journal of General Virology

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Section: Discussionmentioning

confidence: 97%

Sequence and in vitro expression of the M2 gene of turkey rhinotracheitis pneumovirus

Davis

Brown

et al. 1992

Journal of General Virology

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“…The 22K protein is of considerable virological interest, having no counterpart in other paramyxoviruses. In salt and detergent dissociation studies, it is membrane associated (22), but it is not displayed on the surface of mature virus (40). It is nonglycosylated and comprises 194 amino acids (5).…”

Section: Discussionmentioning

confidence: 99%

The 22,000-kilodalton protein of respiratory syncytial virus is a major target for Kd-restricted cytotoxic T lymphocytes from mice primed by infection

Openshaw¹,

Anderson²,

Wertz³

et al. 1990

J Virol

104

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Recombinant vaccinia viruses containing the 22-kilodalton protein (matrixlike or 22K protein) or phosphoprotein gene from respiratory syncytial virus were constructed. These recombinant viruses expressed proteins which were immunoprecipitated by appropriate respiratory syncytial virus antibodies and comigrated with authentic proteins produced by respiratory syncytial virus infection. The new recombinant viruses (and others previously described containing the attachment glycoprotein, fusion, or nucleoprotein genes of respiratory syncytial virus) were used to infect target cells for cultured polyclonal cytotoxic T lymphocytes generated from the spleens of BALB/c or DBA/2 mice primed by intranasal infection with respiratory syncytial virus. Respiratory syncytial virus-specific cytotoxic T lymphocytes (CTL) showed strong Kd (but not Dd)-restricted recognition of the 22K protein. As previously reported, the fusion protein and nucleoprotein were both seen by CTL, but recognition of these proteins was comparatively weak. There was no detectable recognition of other respiratory syncytial virus proteins tested (including phosphoprotein). 22K protein-specific splenic memory CTL persisted for at least 11 months after infection of BALB/c mice. Priming BALB/c mice with recombinant vaccinia virus containing the 22K protein gene induced respiratory syncytial virus-specific memory CTL at lower levels than that previously reported following infection with a similar recombinant containing the fusion protein gene. These data identify the 22K protein as a major target antigen for respiratory syncytial virus-specific CTL from H-2d mice primed by respiratory syncytial virus infection.

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“…The G protein itself is not required for virion attachment as RSV mutant viruses lacking G and/or SH genes have been shown to infect cells likely through interaction with the F protein [38–41]; however, G protein appears to be necessary for efficient virus replication in vivo [40]. Following cell fusion and penetration mediated by the F protein [42], the nucleocapsid is released into the cytoplasm [43–46] where the L protein initiates viral transcription and replication proceeds [47]. Transcription of mRNA occurs in a 3′ to 5′ order from a single promoter near the 3′ end resulting in a series of subgenomic mRNAs [48–52].…”

Section: Rsv Replicationmentioning

confidence: 99%

The Host Response and Molecular Pathogenesis Associated with Respiratory Syncytial Virus Infection

et al. 2009

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Since the isolation of respiratory syncytial virus (RSV) in 1956, its significance as an important human pathogen in infants, the elderly and the immunocompromised has been established. Many important mechanisms contributing to RSV infection, replication and disease pathogenesis have been uncovered; however, there is still insufficient knowledge in these and related areas, which must be addressed to facilitate the development of safe and effective vaccines and therapeutic treatments. A better understanding of the molecular pathogenesis of RSV infection, particularly the host-cell response and transcription profiles to RSV infection, is required to advance disease intervention strategies. Substantial information is accumulating regarding how RSV proteins modulate molecular signaling and regulation of cytokine and chemokine responses to infection, molecular signals regulating programmed cell death, and innate and adaptive immune responses to infection. This review discusses RSV manipulation of the host response to infection and related disease pathogenesis. Keywords adaptive immunity; disease; innate immunity; respiratory syncytial virus Respiratory syncytial virusThe Paramyxoviridae family includes important human respiratory-tract pathogens, of which human respiratory syncytial virus (RSV) is a member. RSV is in the Pneumovirinae subfamily and type species member of the Pneumovirus genus. RSV was first isolated four decades ago from chimpanzees during an outbreak of respiratory illness [1]. Thereafter, RSV was isolated from infants with pneumonia and bronchitis [2], and was named RSV owing to its characteristic †Author for correspondence:

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Expression of the Respiratory Syncytial Virus 22K Protein on the Surface of Infected HeLa Cells

Cited by 18 publications

References 12 publications

Sequence and in vitro expression of the M2 gene of turkey rhinotracheitis pneumovirus

Sequence and in vitro expression of the M2 gene of turkey rhinotracheitis pneumovirus

The 22,000-kilodalton protein of respiratory syncytial virus is a major target for Kd-restricted cytotoxic T lymphocytes from mice primed by infection

The Host Response and Molecular Pathogenesis Associated with Respiratory Syncytial Virus Infection

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