Expression of the QrCPE gene is associated with the induction and development of oak somatic embryos

Valladares, Silvia; Rico, Saleta; Viéitez, A. M.; Covelo, Purificación; Sánchez, Conchi

doi:10.1007/s11295-013-0634-8

Cited by 3 publications

(3 citation statements)

References 50 publications

(43 reference statements)

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“…The expression of the CsCPE gene, that codes for a glycine-rich small polypeptide, was analyzed in both types of lines, showing greater expression in mature tissues [ 69 ]. Further analysis of the gene expression during the induction and development of somatic embryos in chestnut and oak [ 70 , 71 ] suggest a role of the gene in the regeneration ability of these recalcitrant species.…”

Section: Molecular Aspects Of Ar In Chestnutmentioning

confidence: 99%

Recent Advances in Adventitious Root Formation in Chestnut

Vielba

Vidal

José

et al. 2020

Plants

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The genus Castanea includes several tree species that are relevant because of their geographical extension and their multipurpose character, that includes nut and timber production. However, commercial exploitation of the trees is hindered by several factors, particularly by their limited regeneration ability. Regardless of recent advances, there exists a serious limitation for the propagation of elite genotypes of chestnut due to decline of rooting ability as the tree ages. In the present review, we summarize the research developed in this genus during the last three decades concerning the formation of adventitious roots (ARs). Focusing on cuttings and in vitro microshoots, we gather the information available on several species, particularly C. sativa, C. dentata and the hybrid C.sativa × C. crenata, and analyze the influence of several factors on the achievements of the applied protocols, including genotype, auxin treatment, light regime and rooting media. We also pay attention to the acclimation phase, as well as compile the information available about biochemical and molecular related aspects. Furthermore, we considerate promising biotechnological approaches that might enable the improvement of the current protocols.

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Section: Molecular Aspects Of Ar In Chestnutmentioning

confidence: 99%

Recent Advances in Adventitious Root Formation in Chestnut

Vielba

Vidal

José

et al. 2020

Plants

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“…In situ experiments were carried out as previously described (Vielba et al 2011;Valladares et al 2013). The basal part of BS and CR chestnut microshoots treated or not with IBA were collected after 12 h, 10 and 14 d of culture in BRM.…”

Section: In Situ Hybridizationmentioning

confidence: 99%

Expression of a Rap2.12 like-1 ERF gene during adventitious rooting of chestnut and oak microshoots

Valladares¹,

Varas²,

Vielba

et al. 2020

Israel J. Plant Sci.

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Adventitious rooting of cuttings is a complex developmental process in forest species, with several exogenous and endogenous factors influencing the outcome of the process. In this study we applied an in vitro working system, comprising two lines of microshoots with the same genotype but at a different ontogenetic stages, in two different tree species (chestnut and oak). We analyzed the expression of a gene encoding an AP2/ERF transcription factor from group VII in the initial hours of the adventitious rooting induction, both in rooting competent and incompetent microshoots. The analysis revealed that expression of this gene is related to wounding, ontogenetic stage and auxin in a complex and species-specific manner. Putative induction of the gene by auxin was also analyzed in the presence of naphthyl-phthalamic acid (NPA), an auxin transport inhibitor. In situ expression analysis in chestnut relates the gene activity to cambial divisions and root primordia in rooting competent microshoots, as well as in the root apex. The putative role of the gene during adventitious roots formation is discussed.

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“…In the last years, knowledge about gene regulation of plant differentiation has speed out and genes involved on the induction of embryogenic competence of somatic cells have been identified in woody plant species, mainly conifers (Rupps et al., 2016; Gautier et al., 2018). But, in oaks and in spite of its ecological and economical importance, very few reports have been released concerning genetic control of SE induction (Šunderlíková et al., 2009; Valladares et al., 2013) and maturation (Šunderlíková and Wilhem, 2002; Pérez et al., 2015). More recently, DNA demethylation has been associated with SE initiation in Q. alba (Corredoira et al., 2017b).…”

Section: Concluding Remarks and Future Perspectivesmentioning

confidence: 99%

Holm Oak Somatic Embryogenesis: Current Status and Future Perspectives

et al. 2019

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Quercus ilex (holm oak) is one of the most representative trees in the Mediterranean basin, but now the sustainability of its ecosystems is at serious risk due to the lack of natural regeneration and to the presence of a severe disease called oak decline that has caused the death of thousands of trees. The application of biotechnological tools, such as somatic embryogenesis, allows programs of genetic improvement of the species to be speeded up and helps in the conservation of its ecosystems. Somatic embryogenesis is currently considered one of the main biotechnological techniques that has demonstrated significant benefits when has applied to forest tree species, providing significant advantages such as mass propagation, genetic transformation, application of synthetic seed, and cryopreservation of elite genotypes. In this report, the state of the art of somatic embryogenesis in holm oak is reviewed. Factors affecting the induction (plant donor age, type of explant, or plant growth regulators) and maintenance and proliferation of the embryogenic cultures are summarized. Advances on the conversion of somatic embryos into plants and on the acclimatization of these plantlets, as well as the results obtained on the application of the genetic transformation and the cryopreservation procedures to holm oak embryogenic cultures, are also presented.

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Expression of the QrCPE gene is associated with the induction and development of oak somatic embryos

Cited by 3 publications

References 50 publications

Recent Advances in Adventitious Root Formation in Chestnut

Recent Advances in Adventitious Root Formation in Chestnut

Expression of a Rap2.12 like-1 ERF gene during adventitious rooting of chestnut and oak microshoots

Holm Oak Somatic Embryogenesis: Current Status and Future Perspectives

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