Expression of the Psoriasis-associated Antigen, Pso p27, is Inhibited by Cyclosporin A

Abstract

The psoriasis-associated antigen, pso p27, can be isolated from psoriasis scale and is present in complement-activating immune complexes in psoriatic scale, and in serum from patients with psoriasis. The antigen is produced by tryptase-positive cells in the skin lesions and is shown to be a major antigen in the immune reactions in psoriasis. The synthesis of this particular antigen is reduced with the remission of inflammation in the skin lesions. In this study we followed 3 patients with severe plaque psoriasis during treatment with cyclosporin A. In all patients we observed a decrease in the expression of the antigen pso p27 during the therapy. The effectiveness of the therapy varied among the patients, but there was a clear correlation between disease activity and expression of the antigen pso p27 as demonstrated by immunofluorescence in biopsies from selected skin lesions. This observation strengthens our hypothesis that the pso p27 antigen plays an important role in the inflammation in psoriasis.

Psoriasis pathogenesis – Pso p27 constitutes a compact structure forming large aggregates

“…After BN-PAGE, the gel was soaked in NuPAGE transfer buffer (Life Technologies) added 0.1% SDS for 1 h. Proteins were electroblotted onto PVDF membranes using Bio-Rad Mini Trans-Blot Cell and NuPAGE transfer buffer for 1 h at 25 V. The membranes were blocked with 1% Blocking solution (Roche) in PBS and incubated with monoclonal antibody against Pso P27 [16] , [17] and anti-mouse horseradish peroxidase (HRP) conjugated secondary antibody (Dako) as described [5] . Finally 3,3-diaminobenzidine tetrahydrochloride (DAB) (Sigma-Aldrich) was used as immunoblotting substrate.…”

Ethnopharmacological survey of medicinal plants used by patients with psoriasis in the West Bank of Palestine

“…Not surprisingly, patients with psoriasis often seek treatments outside the allopathic paradigm to meet their healthcare needs considering the chronic and frustrating nature of the disease [13–15]. Patients with psoriasis were shown to use different modalities of complementary and alternative medicine (CAM) [14, 16]. The use of medicinal plants has evolved as one of the most preferred CAM modalities across different cultures around the globe [13].…”

“…A considerable number of randomized controlled clinical trials have been conducted using different medicinal plants to treat signs/symptoms of psoriasis [15]. Some medicinal plants were shown to improve signs and symptoms of psoriasis while other did not shown any significant improvements [14, 15, 17]. …”