Expression of the potato leafroll luteovirus coat protein gene in transgenic potato plants inhibits viral infection

Abstract

Transgenic potato plants, cultivar Désirée, were produced that contained the coat protein gene of potato leafroll luteovirus (PLRV). The transformed potato plants expressed the PLRV coat protein (CP) RNA sequences but accumulation of coat protein in transgenic tissues could not be detected. Upon inoculation with PLRV, the PLRV CP RNA expressing potato plants showed a reduced rate of virus multiplication.

Susceptibility of transgenic tobacco plants expressing tobacco rattle virus coat protein to nematode-transmitted and mechanically inoculated tobacco rattle virus

“…However, individual P. pachydermus and groups of 20 trichodorids including P. pachydermus were able to overcome cp-mediated resistance of PLBcp plants as they transmitted TRV isolate PPK20 to the roots of these plants. No other reports are available on resistance of transgenic plants to nematode-transmitted virus but studies on virus transmission by aphids showed that cp-mediated resistance was generally not overcome by aphid vectors (Quemada et al, 1991 ;Van der Wilk et al, 1991). However, results similar to ours were obtained by Lawson et al (1990) who found that some lines of transgenic plants expressing potato virus Y cp were resistant to mechanically inoculated but not to aphidtransmitted virus.…”

Strategies to protect crop plants against viruses: pathogen-derived resistance blossoms.

“…We can now add to these "classic" cases of CPMP several recent examples, particularly among members of the aphidtransmitted potato virus Y (PVY; potyvirus) and luteovirus (PLRV) groups, and the thrip-transmitted TSWV, where intentionally truncated, antisense or nonexpressing (-AUG) CP genes have been transformed into plants and have provided measurable protection or even complete immunity against the appropriate parent virus (50)(51)(52)(53)(54)(55)(56)(57)(58). Recent field tests with the untranslatable tobacco etch virus (TEV) CP RNA lines (50,51) have shown 100%o resistance to high disease pressure (W. G. Dougherty, personal communication).…”

“…Recent field tests with the untranslatable tobacco etch virus (TEV) CP RNA lines (50,51) have shown 100%o resistance to high disease pressure (W. G. Dougherty, personal communication). In all these cases (50)(51)(52)(53)(54)(55)(56)(57)(58), it may be that some direct form of RNA-RNA interference between the transgene transcript and the challenge virus (especially the low titer PLRV) could account for the resistant phenotype. However, with the truncated TEV CP transgenic plants, it was common for the inoculated leaves to develop symptoms and virus titers similar to control plants, but for the plant to outgrow the infection.…”

Short and long distance spread of potato leafroll luteovirus: effects of host genes and transgenes conferring resistance to virus accumulation in potato.

“…Host gene-mediated resistance (H-MR) and transgenemediated resistance (T-MR) in plants transformed with the PLRV coat protein gene have been shown to be effective in decreasing PLRV accumulation in infected potato plants (Barker & Harrison, 1985 ;Kawchuk et al, 1990 ;Van der Wilk et al, 1991 ;Barker et al, 1992 ;Wilson & Jones, 1992). Furthermore, in plants with either of these types of resistance, PLRV-infected cells were found to be restricted largely to internal (adaxial) phloem bundles (Barker & Harrison, 1986 ;Derrick & Barker, 1992).…”