Expression of the platelet‐derived growth factor β receptor during organogenesis and tissue differentiation in the mouse embryo

Abstract

In this study we used in situ hybridization to localize expression of the plateletderived growth factor p (PDGFP) receptor mRNA during organogenesis in the mouse embryo (E 9 . S 16.5). Expression was first seen in periaortic mesenchyme (E 9.5-10.5). Later (E 12.5-E 16.51, the receptor was expressed in the mesenchymal component of many developing tissues and organs, particularly derivatives of the primitive gut. The expression was exceptionally high in mesenchyme directly supporting an epithelium, typical of many developing organs such as the trachea and intestine. However, as the mesenchyme differentiated into smooth muscle, PDGFp receptor mRNA was no longer detected. The expression of the PDGFp receptor mRNA in mesenchymal components of developing organs, along with its absence in epithelial tissues, indicates that it may play a role in mesenchymal-epithelial interactions during organ development. Somewhat unexpectedly, the PDGFP receptor was highly expressed in the endothelium of small blood vessels and vascular structures such as the hyaloid plexus and choroid plexus. In large blood vessels, PDGFp receptor mRNA was found in the mesenchyme surrounding the endothelium. This suggests that the PDGFp receptor is involved in growth and development of blood vessels. o

Abnormal kidney development and hematological disorders in PDGF beta-receptor mutant mice.

“…These results are similar to a recent in situ hybridization study (Shinbrot et al 1994). Some of these locations also show expression of the aR, but the general pattems of expression differ significantly.…”

Comparative study assessing effects of sonic hedgehog and VEGF in a human co-culture model for bone vascularisation strategies

“…Effects of Shh and VEGF on bone vascularisation smooth muscle cells (Zerwes and Risau, 1987;Shinbrot et al, 1994;Lindahl et al, 1997). Additionally, PDGF-BB also induces the differentiation of undifferentiated mesenchymal cells towards a mural cell fate (Hellstrom et al, 1999;Hirschi et al, 1999).…”

PDGF function in diverse neural crest cell populations

“…50 Like PDGFRα, PDGFRβ is expressed in many mesenchymal cells throughout the embryo including cardiac NCCs. 44,51 Once cardiac NCCs reach the outflow tract a segregation of PDGF receptor expression occurs, where PDGFRα is associated with the tunica adventitia and mesenchyme throughout the aortic arch and PDGFRβ is expressed predominantly in the neural crest and non-neural crest-derived vascular smooth muscle cell population.…”