Expression of the P2X₂receptor in different classes of ileum myenteric neurons in the female obese ob/ob mouse

Abstract

We demonstrate increases in P2X₂R expression and alterations in nNOS, ChAT and CalR IR in ileal myenteric neurons of female ob/ob mice compared to wild-type controls.

Co-localization of Pirt protein and P2X2 receptors in the mouse enteric nervous system

“…In our previous work, double-labeling experiments demonstrated that about 10-25 % of neurons with P2X2 immunoreactivity in the myenteric plexus and 30-50 % in the submucosal plexus expressed Calbindin, a marker for intrinsic sensory neurons [11]. Co-expression of P2X2 with neuronal nitric oxide synthase, choline acetyltransferase, and Calretinin in neurons of the small intestine myenteric plexus was detected [30] [12]. While one can speculate on the overlap of P2X2-ir/Pirt-ir cells with the above cell types, more information is needed to identify the cell type for a better understanding of the role of Pirt protein in the gut.…”

Effects of aging on the architecture of the ileocecal junction in rats

“…Furness [26] (2006) emphasized that NOS catalyzes the formation of the NO that is present in the myenteric plexus and neuronal processes in the gut, which acts to relax the muscle fiber function of the gastrointestinal musculature. Previous studies have shown the presence of NOS in the enteric ganglia and muscle fibers under various conditions, such as malnutrition and renutrition [27,28] , ischemia and reperfusion [29][30][31] and obesity [32,33] . The enteric neurons of the ICJ stain positive for NOS, PGP 9.5, and c-kit [3,4,6,8] .…”

Papel do antagonista Brilliant Blue G sobre os neurônios mioentéricos imunorreativos ao receptor P2X7 do íleo de ratos submetidos à isquemia/reperfusão intestinal.

“…Essencialmente, os plexos entéricos seguem um padrão ao longo do trato digestório, porém, diferenças quanto à densidade e ao tamanho dos neurônios, bem como a forma dos gânglios, podem ser encontradas no mesmo segmento do trato digestório dos animais de mesma espécie e com diferentes idades (MATINI;MAYER;PELLEGRINI, 1997;MCKEOWN;CHOW;YOUNG, 2001;QU et al, 2008), ou submetidos à condições experimentais, como a inflamação (BOYER et al, 2005;DE GIORGIO et al, 2004), desnutrição (CASTELUCCI et al, 2002bGIROTTI et al, 2013;GOMES et al, 2006;GREGGIO et al, 2010;MISAWA et al, 2010), isquemia intestinal (PAULINO et al, 2011; PALOMBIT et al, 2013) e obesidade (MIZUNO et al, 2012).…”

“…Em animais ob/ob foi verificada diminuição na densidade dos neurônios NOS-ir e ChAT-ir (MIZUNO et al, 2012) e em protocolos de I/R foram vistas alterações no número de neurônios no plexo mioentérico (BOBNA, 2011;PALOMBIT et al, 2013;PAULINO et al, 2011).…”