Expression of the neonatal Fc receptor, FcRn, on human intestinal epithelial cells

Israel, Esther J.; Taylor, Shirley M.; Wu, Zezhou; Mizoguchi, Emiko; Blumberg, Richard S.; Bhan, Atul K.; Simister, Neil E.

doi:10.1046/j.1365-2567.1997.00326.x

Cited by 294 publications

(232 citation statements)

References 25 publications

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“…Costaining with PECAM-1 showed little colocalization with FcRn in the gut. In contrast to a previous report using human tissues, we did not detect FcRn expression in colonic enterocytes or lamina propria cells (data not shown) (38). …”

Section: Fcrn Expression In the Adult Gutcontrasting

confidence: 99%

Neonatal FcR Expression in Bone Marrow-Derived Cells Functions to Protect Serum IgG from Catabolism

Akilesh

Christianson

Roopenian

et al. 2007

The Journal of Immunology

230

219

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The neonatal FcR (FcRn) is a receptor that protects IgG from catabolism and is important in maintaining high serum Ab levels. A major site of expression of FcRn is vascular endothelial cells where FcRn functions to extend the serum persistence of IgG by recycling internalized IgG back to the surface. Because FcRn is expressed in other tissues, it is unclear whether endothelial cells are the only site of IgG protection. In this study, we used FcRn-deficient mice and specific antiserum to determine the tissue distribution of FcRn in the adult mouse. In addition to its expression in the vascular endothelium of several organs, we found FcRn to be highly expressed in bone marrow-derived cells and professional APCs in different tissues. Experiments using bone marrow chimeras showed that FcRn expression in these cells acted to significantly extend the half-life of serum IgG indicating that in addition to the vascular endothelium, bone marrow-derived phagocytic cells are a major site of IgG homeostasis.

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Section: Fcrn Expression In the Adult Gutcontrasting

confidence: 99%

Neonatal FcR Expression in Bone Marrow-Derived Cells Functions to Protect Serum IgG from Catabolism

Akilesh

Christianson

Roopenian

et al. 2007

The Journal of Immunology

230

219

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“…Frozen sections of fetal intestinal mucosa showed distinct villous and crypt staining patterns similar to that previously described (7). Villous enterocytes showed a delicate punctate staining located at the apical membrane.…”

Section: Immunohistochemical Analysissupporting

confidence: 79%

Distribution of the IgG Fc Receptor, FcRn, in the Human Fetal Intestine

Shah¹,

Dickinson²,

Blumberg³

et al. 2003

Pediatric Research

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The intestinal Fc receptor, FcRn, functions in the maternofetal transfer of gamma globulin (IgG) in the neonatal rodent. In humans, most of this transfer is presumed to occur in utero via the placenta. Although the fetus swallows amniotic fluid that contains immunoglobulin, it is unknown whether this transfer also occurs via the fetal intestine. A human FcRn has been identified in the syncytiotrophoblast that mediates the maternofetal transfer of antibody. It has also been identified in human fetal intestine and is postulated to function in IgG transport. We hypothesize that the human fetal intestinal FcRn may play a role in IgG transport from the amniotic fluid into the fetal circulation. The aim of this study was to characterize the distribution of the FcRn along the human fetal intestine. Lysates prepared from human fetal intestine and from a nonmalignant human fetal intestinal epithelial cell line (H4) were subjected to Western blot analysis and probed using anti-FcRn antibodies. A 42-kD band, consistent with the known molecular weight of the FcRn, was detected along the human fetal intestine and in H4 cells. Expression of the human FcRn was confirmed with immunohistochemistry. Our study demonstrates the expression of FcRn along the human fetal intestine and in a human nonmalignant fetal intestinal epithelial cell line (H4), which by location indicates that FcRn could play a role in the uptake and transport of IgG in the human fetus.Humoral immunity acquired via maternofetal transfer of antibody is critical in the prevention of perinatal infections. In rodents, this transfer occurs through the yolk sac and the intestine via a Fc receptor for IgG (FcRn). This receptor has been considered important in the maternofetal transmission of IgG in the yolk sac of mice and rats and also in the human placental syncytiotrophoblast (1). The FcRn is also expressed in the small intestine of suckling mice and rats, where the receptor functions in the uptake of IgG from ingested maternal milk. Although a FcRn has been demonstrated on the human fetal small intestine, a similar function for this receptor in the human fetal intestine has not been demonstrated to date.The acquisition of humoral immunity early in life may have a significant lifelong impact on the prevention of infectious and inflammatory diseases. Because IgG synthesis in the human fetus is reduced, most IgG in the blood of newborns is maternal in origin. Low levels of IgG

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“…Young mammals need some time after birth until they can produce their own protecting antibodies, for example against common bacteria epitopes. In the meantime, they acquire the spectrum of antibodies present in maternal serum by one or both of the following ways: (a) in utero transplacental antibody trans- port (active in humans but less efficient in mice (42)) and/or (b) postnatal import of maternal antibodies present in milk via an antibody transport system in the gut epithelium (active in mice but less in humans) (42,43). The induction of an Eda-deficient phenotype in pups of wild type mothers receiving antibodies intravenously shows that enough antibody was transported to inhibit the developmental function of EDA at relatively early time points, because EDAR expression in the oral epithelium starts at embryonic day 10 (7).…”

Section: Discussionmentioning

confidence: 99%

Generation and Characterization of Function-blocking Anti-ectodysplasin A (EDA) Monoclonal Antibodies That Induce Ectodermal Dysplasia

Kowalczyk‐Quintas

Willen

Dang

et al. 2014

Journal of Biological Chemistry

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Expression of the neonatal Fc receptor, FcRn, on human intestinal epithelial cells

Cited by 294 publications

References 25 publications

Neonatal FcR Expression in Bone Marrow-Derived Cells Functions to Protect Serum IgG from Catabolism

Neonatal FcR Expression in Bone Marrow-Derived Cells Functions to Protect Serum IgG from Catabolism

Distribution of the IgG Fc Receptor, FcRn, in the Human Fetal Intestine

Generation and Characterization of Function-blocking Anti-ectodysplasin A (EDA) Monoclonal Antibodies That Induce Ectodermal Dysplasia

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