Abstract:To actively express an outer membrane protein, protein I (PI), from different strains of Neisseria gonorrhoeae in E. coli, PI gene fragments from two reference strains and four clinical isolates of Neisseria gonorrhoeae were obtained with PCR amplification. They were cloned into the PCR cloning vector pBS-T to form pBS-T-PI and sequenced. Subsequently, they were cloned into an expression vector pET-30b (+) to generate pET-PI recombinants. After inducing with isopropyl-b-D-thiogalactopyranoside (IPTG), the expr… Show more
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