Expression of the negative co-stimulatory ligand sCD152 in the yeast, Pichia pastoris, and its regulation of antigen specific immune responses

Zhu, Nianyong; Gao, Neng; Wang, YingMing; Wang, Xiaohua; Yang, Yi

doi:10.1016/j.intimp.2003.11.007

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2009

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“…sCD152 was produced by engineering Pchia pastoris GS115 yeast, 26 and sCD95L was produced by engineering Escherichia coli in our laboratory (purity > 95%, data not shown). sCD152 was produced by engineering Pchia pastoris GS115 yeast, 26 and sCD95L was produced by engineering Escherichia coli in our laboratory (purity > 95%, data not shown).…”

Section: Experimental Groups and Protocolmentioning

confidence: 96%

“…There were 10 mice allocated to the sCD152, sCD95L, sCD152 + sCD95L, natural saline, and sCD152 + sCD95L + mIL-10 + hTGF-b groups, and 6 to the ciclosporin and the mIL-10 + hTGF-b groups. sCD152 was produced by engineering Pchia pastoris GS115 yeast, 26 and sCD95L was produced by engineering Escherichia coli in our laboratory (purity > 95%, data not shown). Ciclosporin was purchased from Novartis Inc (Basel, Switzerland) and mIL-10 and hTGF-b were purchased from PeproTech Ltd (Rehovot, Israel).…”

Section: Experimental Groups and Protocolmentioning

confidence: 96%

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