Expression of the muscle regulatory factor MRF4 during somite and skeletal myofiber development

Hinterberger, Timothy J.; Sassoon, David; Rhodes, Simon J.; Konieczny, Stephen F.

doi:10.1016/s0012-1606(05)80014-4

Cited by 272 publications

(182 citation statements)

References 32 publications

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“…Various studies at the mRNA level have identified few, if any, transcripts of MyoD and myogenin in uninjured, adult muscle (Hinterberger et al, 1991;Eftimie et al, 1991;Buonanno et al, 1992;Beilharz et al, 1992). However, conclusions from in vivo studies regarding the nature of entities expressing MyoD and myogenin at the mRNA or protein levels following overt injury or other types of stress seem to vary depending on the model used.…”

Section: How Do the Results With The Temporal Expression Of Myogenic mentioning

confidence: 99%

Temporal expression of regulatory and structural muscle proteins during myogenesis of satellite cells on isolated adult rat fibers

Yablonka–Reuveni

Rivera

1994

Developmental Biology

400

374

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Myogenic precursors in adult skeletal muscle (satellite cells) are mitotically quiescent but can proliferate in response to a variety of stresses including muscle injury. To gain further understanding of adult myoblasts, we analyzed myogenesis of satellite cells on intact fibers isolated from adult rat muscle. In this culture model, satellite cells are maintained in their in situ position underneath the fiber basement membrane. In the present study patterns of satellite cell proliferation, expression of myogenic regulatory factor proteins, and expression of differentiationspecific, cytoskeletal proteins were determined, via immunohistochemistry of cultured fibers. The temporal appearance and the numbers of cells positive for proliferating cell nuclear antigen (PCNA) or for MyoD were similar, suggesting that MyoD is present in detectable amounts in proliferating but not quiescent satellite cells. Satellite cells positive for myogenin, α-smooth muscle actin (αSMactin), or developmental sarcomeric myosin (DEVmyosin) appeared following the decline in PCNA and MyoD expression. However, expression of myogenin and αSMactin was transient, while DEVmyosin expression was continuously maintained. Moreover, the number of DEVmyosin+ cells was only half of the number of myogenin+ or αSMactin+ cells-indicating, perhaps, that only 50% of the satellite cell descendants entered the phase of terminal differentiation. We further determined that the number of proliferating satellite cells can be modulated by basic FGF but the overall schedule of cell cycle entry, proliferation, differentiation, and temporal expression of regulatory and structural proteins was unaffected. We thus conclude that satellite cells conform to a highly coordinated program when undergoing myogenesis at their native position along the muscle fiber.

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Section: How Do the Results With The Temporal Expression Of Myogenic mentioning

confidence: 99%

Temporal expression of regulatory and structural muscle proteins during myogenesis of satellite cells on isolated adult rat fibers

Yablonka–Reuveni

Rivera

1994

Developmental Biology

400

374

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“…This was surprising as MRF4 was less sensitive to modulation by denervation [5,6]. From these studies and the observations that MRF4 is expressed later in limb bud development [12] and is present at higher levels in postnatal muscle [5] than other MRF transcripts, particularly myogenin, it has been postulated that MRF4 has a crucial role in preserving the differentiated state rather than regulating muscle fibre phenotype. Our results suggest that there may be a more complex role for this particular MRF in modulating adult muscle gene expression.…”

Section: Discussionmentioning

confidence: 99%

Two myogenic regulatory factor transcripts exhibit muscle‐specific responses to disuse and passive stretch in adult rats

Loughna

Brownson

1996

FEBS Letters

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Levels of myogenic regulatory factor (MRF) transcripts are altered in a muscle-specific manner in response to hind limb immobilisation of adult male rats, for a 2 day period, in either a lengthened or shortened position which result in passive stretch or disuse atrophy respectively. Myogenin transcript levels were dramatically elevated in the stretched plantaris but not soleus, whereas the MRF4 transcript was significantly elevated in soleus but not plantaris. Levels of myogenin mRNA were unaffected by disuse in either muscle and MRF4 was markedly lower in plantaris in response to disuse.

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“…These studies together with studies of myogenic cells in vitro, which express different combinations of the bHLH family, have raised the possibility that each member of the family may differentially activate a distinct subset of muscle-specific genes, or may be expressed in distinct myoblast or myocyte populations (Miller, 1990(Miller, , 1991Hinterberger et al, 1991). However, in this paper we have identified a differentiation-specific program of gene expression independent of future (or past) phenotype that is found in myotubes derived from primary, secondary, and adult myoblasts.…”

Section: Helix-loophelix Regulatory Proteins and The Differentiation mentioning

confidence: 99%

Identification of a program of contractile protein gene expression initiated upon skeletal muscle differentiation

Sutherland

Esser

Elsom

et al. 1993

Developmental Dynamics

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The functional diversity of skeletal muscle is largely determined by the combinations of contractile protein isoforms that are expressed in different fibers. Just how the developmental expression of this large array of genes is regulated to give functional phenotypes is thus of great interest. In the present study, we perform a comprehensive analysis of contractile protein isoform mRNA profiles in skeletal muscle systems representing each generation of fiber formed: primary, secondary, and regenerating fibers. We find that in each system examined there is a common pattern of isoform gene expression during early differentiation for 5 of the 6 gene families we have investigated: myosin light chain (MLCI1, MLC2, tropomyosin, troponin (Tn)C, and TnI. We suggest that the common isoform patterns observed together represent a genetic program of skeletal muscle differentiation that is independent of the mature fiber phenotype and is found in all newly formed myotubes. Within each of these contractile protein gene families the program is independent of the isoforms of myosin heavy chain (MHC) expressed. The maintenance of such a program may reflect a specific requirement of the initial differentiation process. 0 1993 Wiley-Liss, Inc.

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Expression of the muscle regulatory factor MRF4 during somite and skeletal myofiber development

Cited by 272 publications

References 32 publications

Temporal expression of regulatory and structural muscle proteins during myogenesis of satellite cells on isolated adult rat fibers

Temporal expression of regulatory and structural muscle proteins during myogenesis of satellite cells on isolated adult rat fibers

Two myogenic regulatory factor transcripts exhibit muscle‐specific responses to disuse and passive stretch in adult rats

Identification of a program of contractile protein gene expression initiated upon skeletal muscle differentiation

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