Expression of the mouse testicular histone gene H1t during spermatogenesis

Drabent, Birgit; Bode, Christa; Bramlage, Birgit; Doenecke, Detlef

doi:10.1007/bf02484408

Cited by 107 publications

(97 citation statements)

References 26 publications

(27 reference statements)

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“…Some histone variants such as testis specific histone H2B variant, TH2B and testis specific histone H1, H1t are present from early spermatogenic cells to the round spermatid stage [67], [68], [69]. However, variants of H2A such as H2AL1/2, H2A.Bbd, or of H1, such as H1t2 and Hils1, are specifically incorporated during the histone-to-protamine exchange in round/elongating spermatids [70], [71], [72], [73].…”

Section: Global Chromatin Remodeling During Spermiogenesismentioning

confidence: 99%

Parental epigenetic control of embryogenesis: a balance between inheritance and reprogramming?

Gill

Erkek

Peters

2012

Current Opinion in Cell Biology

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At fertilization, fusion of two differentiated gametes forms the zygote that is capable of forming all of the varied cell lineages of an organism. It is widely thought that the acquisition of totipotency involves extensive epigenetic reprogramming of the germline state into an embryonic state. However, recent data argue that this reprogramming is incomplete and that substantial epigenetic information passes from one generation to the next. In this review we summarize the changes in chromatin states that take place during mammalian gametogenesis and examine the evidence that early mammalian embryogenesis may be affected by inheritance of epigenetic information from the parental generation.

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Section: Global Chromatin Remodeling During Spermiogenesismentioning

confidence: 99%

Parental epigenetic control of embryogenesis: a balance between inheritance and reprogramming?

Gill

Erkek

Peters

2012

Current Opinion in Cell Biology

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“…Expression of Hsp70-2, Clgn (encoding calmegin), HoxA4, H1f3 (encoding histone H1t), Sprm-1, and Crem genes was reduced in Cgt Ϫ/Ϫ mice. These genes are reported to be expressed in primary spermatocytes at the late pachytene stage (25)(26)(27)(28)(29)(30). Expression of Ccna1 (encoding cyclin A1) gene is reported to occur at the end of prophase of meiosis I (31).…”

Section: Arrest Of Genetic Program Of Spermatogenesis In Cgt-deficienmentioning

confidence: 99%

Requirement of Seminolipid in Spermatogenesis Revealed by UDP-galactose:Ceramide Galactosyltransferase-deficient Mice

Fujimoto¹,

Tadano-Aritomi²,

Tokumasu³

et al. 2000

Journal of Biological Chemistry

101

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Although seminolipid has long been suspected to play an essential role in spermatogenesis because of its uniquely abundant and temporally regulated expression in the spermatocytes, direct experimental evidence has been lacking. We have tested the hypothesis by examining the testis of the UDP-galactose:ceramide galactosyltransferase-deficient mouse, which is incapable of synthesizing seminolipid. Spermatogenesis in homozygous affected males is arrested at the late pachytene stage and the spermatogenic cells degenerate through the apoptotic process. This stage closely follows the phase of rapid seminolipid synthesis in the wild-type mouse. These observations not only provide the first experimental evidence that seminolipid is indeed essential for normal spermatogenesis but also support the broader concept that cell surface glycolipids are important in cellular differentiation and cell-to-cell interaction.Seminolipid (3-sulfogalactosyl-1-alkyl-2-acyl-sn-glycerol) is the principal glycolipid in spermatozoa of mammals comprising, for example, approximately 3% of total lipids and more than 90% of total glycolipids in boar spermatozoa (1-3). During spermatogenesis, seminolipid is synthesized rapidly in the early phase of spermatocyte development and maintained in subsequent germ cell stages (4 -6). This developmentally regulated rapid synthesis suggested a specific and possibly essential function of seminolipid in spermatogenesis (7) but experimental evidence has been lacking. Firm evidence in support of the speculation would have important bearing to the general concept that cell surface glycoconjugates are important in cellular differentiation, and cell-to-cell interaction (8).Seminolipid is synthesized by sulfation of its precursor, galactosylalkylacylglycerol (GalEAG) 1 . GalEAG is synthesized by UDPgalactose:ceramide galactosyltransferase (CGT, EC 2.4.1.62), which, besides GalEAG, also synthesizes the major myelin galactolipid, galactosylceramide (GalCer), galactosylsphingosine (psychosine), and galactosyldiacylglycerol (GalAAG) (9, 10). The CGTdeficient mice recently generated by gene-targeting do not synthesize any of these products and subsequent derivatives of the products (11)(12)(13)(14). Thus, the CGT-deficient mouse is an ideal experimental model to examine the consequences of lack of seminolipid to spermatogenesis. This report describes the first definitive evidence that deficient seminolipid biosynthesis indeed causes devastating disruption of the normal spermatogenetic process. EXPERIMENTAL PROCEDURESMice-The mice heterozygous for the disrupted Cgt gene (11) were originally supplied by Dr. B. Popko and maintained by backcrossing to C57BL/6N. Genotype was determined according to Coetzee et al. (11). WBB6F1 Kit W/W-v and WBB6F1 Mg f Sl/Sl-d mutant mice were purchased from Japan SLC, Inc., and C57BL/6N inbred mice were purchased from CLEA Japan, Inc. Isolation of Testicular Germ Cells-Testicular germ cells were isolated from decapsulated testes of sexually mature male C57BL/6N mice (15).RT-PCR Analysis-RNA...

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“…Ubiquitinated histone H3 and testes-specific H3 (TH3) were first identified in a study of rat spermatogenesis, with the highest level in elongated spermatids (Chen et al, 1998). Ubiquitination may be required for somatic histones replacement by testis specific histones during meiosis (Poccia et al, 1987;Meistrich, 1989;Drabent et al, 1996) and for the subsequent substitution of most histones by transition proteins, which are later replaced by protamines during spermiogenesis (Mills et al, 1977;Meistrich, 1989). This latter substitution of histones is critical for normal chromatin condensation in spermatids.…”

Section: Introductionmentioning

confidence: 99%

Regulated expression of the ubiquitin protein ligase, E3^Histone/LASU1/Mule/ARF‐BP1/HUWE1, during spermatogenesis

Liu

Miao

Xia

et al. 2007

Developmental Dynamics

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A ubiquitin protein ligase (E3), E3Histone /LASU1 (Mule/ARF-BP1/HUWE1), was recently identified that mediates ubiquitination of core histones, the Mcl-1 anti-apoptotic protein, and the p53 tumor suppressor protein. However, the expression of E3 Histone /LASU1 remains poorly studied. Because we identified E3 Histone /LASU1 from the testis, we explored its regulation during spermatogenesis. In the first wave of rat spermatogenesis, E3Histone /LASU1 mRNA and protein had peak expression at days 10 and 20, respectively, and decreased with age. Consistent with these findings, immunohistochemistry revealed that E3 Histone /LASU1 was highly expressed in nuclei from spermatogonia to mid-pachytene spermatocytes. There was no obvious staining in spermatids, when histones are ubiquitinated and degraded.

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Expression of the mouse testicular histone gene H1t during spermatogenesis

Cited by 107 publications

References 26 publications

Parental epigenetic control of embryogenesis: a balance between inheritance and reprogramming?

Parental epigenetic control of embryogenesis: a balance between inheritance and reprogramming?

Requirement of Seminolipid in Spermatogenesis Revealed by UDP-galactose:Ceramide Galactosyltransferase-deficient Mice

Regulated expression of the ubiquitin protein ligase, E3^Histone/LASU1/Mule/ARF‐BP1/HUWE1, during spermatogenesis

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Expression of the mouse testicular histone gene H1t during spermatogenesis

Cited by 107 publications

References 26 publications

Parental epigenetic control of embryogenesis: a balance between inheritance and reprogramming?

Parental epigenetic control of embryogenesis: a balance between inheritance and reprogramming?

Requirement of Seminolipid in Spermatogenesis Revealed by UDP-galactose:Ceramide Galactosyltransferase-deficient Mice

Regulated expression of the ubiquitin protein ligase, E3Histone/LASU1/Mule/ARF‐BP1/HUWE1, during spermatogenesis

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Regulated expression of the ubiquitin protein ligase, E3^Histone/LASU1/Mule/ARF‐BP1/HUWE1, during spermatogenesis