Expression of the mouse interleukin‐2 receptor γ chain in various cell populations of the thymus and spleen

Kondo, Motonari; Ohashi, Y; Tada, Kotaro; Nakamura, Masataka; Sugamura, Kazuo

doi:10.1002/eji.1830240914

Cited by 49 publications

(25 citation statements)

References 39 publications

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“…Neutralizing anti-c-Kit (ACK2) and neutralizing anti-IL-7R␣ (A7R34) antibodies were kind gifts from S. Nishikawa (Kyoto University, Japan). Because virtually all thymocytes express the common cytokine receptor ␥ chain, thymocytes that express the IL-7R␣ chain should possess a functional IL-7R heterodimer (13). These antibodies were directly conjugated with phycoerythrin (PE), fluorescein-5-isothiocyanate (FITC), allophycocyanin, or Texas Red.…”

Section: Methodsmentioning

confidence: 99%

Two distinct pathways of positive selection for thymocytes

Akashi

Kondo

Weissman

1998

Proc. Natl. Acad. Sci. U.S.A.

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These data raise the possibility of biological and developmental differences during positive selection between the cKit ϩ and c-Kit Ϫ pathways. We delineate these two pathways by evaluating the expression of IL-7R as well as c-Kit, time course, cell cycle status, and contribution to generation of SP progeny. Glimcher (Harvard School of Public Health). MHC-class I and II double knockout (MHC-DKO) mice were obtained by intercrossing these mice strains. All of these mice were used between 3 and 5 weeks of age. MATERIALS AND METHODS MiceCell Sorting and Analysis. The antibodies used in immunofluorescence staining included AL1-4A2 (anti-Ly5

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Section: Methodsmentioning

confidence: 99%

Two distinct pathways of positive selection for thymocytes

Akashi

Kondo

Weissman

1998

Proc. Natl. Acad. Sci. U.S.A.

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“…The ␤ and ␥ chains, which are shared with other interleukin receptors (4,5), belong to the family of hematopoietic cytokine receptors. Resting T cells constitutively express the ␥ chain (6,7) and basal levels of the ␤ chain (8). IL-2R␤ expression increases upon antigen stimulation (9), 2 .…”

Section: Interleukin-2 (Il-2)mentioning

confidence: 99%

Interaction of STAT5 Dimers on Two Low Affinity Binding Sites Mediates Interleukin 2 (IL-2) Stimulation of IL-2 Receptor α Gene Transcription

Meyer¹,

Reichenbach²,

Schindler³

et al. 1997

Journal of Biological Chemistry

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“…These findings are consistent with previously published data on the expression of CD122 in mouse lymphoid tissues. 19,20 In fsn/fsn PLN, CD122 was expressed at low levels by a small minority of CD4 + cells. However, the most striking and consistent feature of fsn/fsn PLN was the dramatically increased expression of CD122 on CD8 + cells.…”

Section: Expression Of Cd122mentioning

confidence: 95%

Dysregulated expression of CD69 and IL‐2 receptor α and β chains on CD8⁺ T lymphocytes in flaky skin mice

Abernethy¹,

Hagan

Tan³

et al. 2000

Immunol Cell Biol

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Summary T-cell activation is considered to be an important element in the pathogenesis of psoriasis, a human skin disease characterized by keratinocyte hyperproliferation, altered keratinocyte differentiation and inflammation of the dermis and epidermis. Mice homozygous for the flaky skin (fsn) mutation develop a skin disorder that has histopathological and biochemical features resembling some forms of psoriasis. It has been reported recently that peripheral lymph nodes (PLN) in fsn/fsn mice exhibit various abnormalities in T-cell development suggestive of dysregulated T-and B-cell activation. In the present study, the expression of the inducible T-cell activation antigens CD69 and IL-2 receptor α chain (CD25) on PLN cells from fsn/fsn mice and their phenotypically normal littermates is examined. Expression of CD69 was significantly increased on PLN cells in fsn/fsn mice (mean ± SD, 49.9 ± 14.7% of cells) compared with control mice (14.6 ± 4.2 %). Analysis of CD4 + and CD8 + T cell subsets revealed that expression of CD69 in fsn/fsn PLN was significantly biased toward CD8 + cells. Although expression of CD25 was preferentially associated with CD4 + rather than CD8 + cells in both fsn/fsn and control PLN, with most CD4 + CD25 + cells being CD25 hi , the proportion of CD4 + cells expressing CD25 was higher in fsn/fsn than control PLN. In contrast, CD25 was expressed by 2-3 % of CD8 + PLN cells in both fsn/fsn and control mice and CD25 hi cells accounted for < 1 % of CD8 + cells in fsn/fsn PLN. The paucity of CD25 on CD8 + cells in fsn/fsn PLN did not appear to be due to a defect in the ability of these cells to upregulate CD25, because T cell receptor stimulation in vitro induced high expression of CD25 on both CD4 + and CD8 + cells. A striking and consistent finding was that most CD8 + cells in fsn/fsn PLN expressed high levels of IL-2R β chain (CD122). In contrast, CD122 was expressed at low levels on CD8 + cells in control mice. Analysis of PLN cells from newborn fsn/fsn mice revealed that the high expression of CD122 on CD8 + cells was established by 2 weeks of age, prior to the appearance of clinical skin disease. These data indicate that large numbers of T cells in fsn/fsn mice are activated and reinforce the view that fsn is an important regulator of lymphocyte development and function. The relationship between T-cell activation and flaky skin disease in these mice remains to be established.

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Expression of the mouse interleukin‐2 receptor γ chain in various cell populations of the thymus and spleen

Cited by 49 publications

References 39 publications

Two distinct pathways of positive selection for thymocytes

Two distinct pathways of positive selection for thymocytes

Interaction of STAT5 Dimers on Two Low Affinity Binding Sites Mediates Interleukin 2 (IL-2) Stimulation of IL-2 Receptor α Gene Transcription

Dysregulated expression of CD69 and IL‐2 receptor α and β chains on CD8⁺ T lymphocytes in flaky skin mice

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Expression of the mouse interleukin‐2 receptor γ chain in various cell populations of the thymus and spleen

Cited by 49 publications

References 39 publications

Two distinct pathways of positive selection for thymocytes

Two distinct pathways of positive selection for thymocytes

Interaction of STAT5 Dimers on Two Low Affinity Binding Sites Mediates Interleukin 2 (IL-2) Stimulation of IL-2 Receptor α Gene Transcription

Dysregulated expression of CD69 and IL‐2 receptor α and β chains on CD8+ T lymphocytes in flaky skin mice

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Dysregulated expression of CD69 and IL‐2 receptor α and β chains on CD8⁺ T lymphocytes in flaky skin mice