Expression of the melanoma-associated antigen is associated with progression of human thyroid cancer

Liu, Wei; Mercado, Moisés; Ezzat, Shereen; Sylvia, L.

doi:10.1677/erc-09-0002

Cited by 18 publications

(16 citation statements)

References 26 publications

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“…It has been reported previously that MAGE-A proteins can be found in different cell compartments, including the nucleus (10), cytoplasm (35), and PML nuclear bodies (12). We observed here that endogenous MageB2, in addition to nuclear and cytoplasm fractions, is also present in the nucleolar fraction (Fig.…”

Section: P53-independent Effect Of Mageb2 On Cell Proliferation-wesupporting

confidence: 80%

Human MageB2 Protein Expression Enhances E2F Transcriptional Activity, Cell Proliferation, and Resistance to Ribotoxic Stress

Peche

Ladelfa

Toledo

et al. 2015

Journal of Biological Chemistry

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Background: MageB2 is a tumor-specific antigen with unknown function. Results: Through a mechanism involving histone deacetylases, MageB2 enhances E2F activity and resistance to Actinomycin D. Conclusion: MageB2 is a protein conferring proliferation properties and resistance to ribotoxic stress to tumor cells. Significance: Expression of MageB2 in human tumors could be a marker of chemotherapy refraction.

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Section: P53-independent Effect Of Mageb2 On Cell Proliferation-wesupporting

confidence: 80%

Human MageB2 Protein Expression Enhances E2F Transcriptional Activity, Cell Proliferation, and Resistance to Ribotoxic Stress

Peche

Ladelfa

Toledo

et al. 2015

Journal of Biological Chemistry

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“…6A,B). Staining intensity and distribution scores were derived from software image analyses as previously described (Cheng et al, 2009,Cheng et al, 2011). This approach identified a positive staining score correlating Grb14 positivity and the presence of lymph node metastases (Fig.…”

Section: Resultsmentioning

confidence: 99%

“…Immunohistochemical detection of Grb14 was standardized using positive and negative controls according to the manufacturer (Santa Cruz) and assessed under light microscopy by an endocrine pathologist to confirm appropriate tissue reactivity of the stain. Immunoreactivities were then evaluated by automated analysis using specific algorithms for each compartment as previously described (Cheng et al, 2009,Cheng et al, 2011). …”

Section: Methodsmentioning

confidence: 99%

The insulin resistance Grb14 adaptor protein promotes thyroid cancer ret signaling and progression

et al. 2011

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The growth factor receptor-bound protein (Grb) 14 is an adapter molecule of the Grb7/10/14 family with characteristic BPS domains serving to avidly bind tyrosine kinases. Grb14 inhibits insulin receptor (IR) catalytic activity through interaction with the BPS domain and impedes peptide substrate binding. Members of this Grb family have also been shown to interact with other kinases through their SH2 domain. Here we examined the functional role of Grb14 in thyroid cancer using loss- and gain-of-function approaches. Stable knockdown of Grb14 in thyroid cancer cells facilitated insulin receptor signaling. In contrast, RET phosphorylation was diminished in concert with reduced activation of Akt and STAT3. Loss of Grb14 also resulted in diminished cell proliferation and invasion both in vitro and in mouse flank xenografts. In complementary studies, forced expression of Grb14 interrupted insulin receptor signaling but facilitated RET activation, STAT3, and Akt phosphorylation. Consistent with these findings Grb14 over-expression enhanced cell invasion and resulted in striking metastases in an orthotopic thyroid cancer mouse xenograft model. Primary human thyroid cancer microarrays revealed a positive correlation between Grb14 expression and invasive behavior. Our findings uncover a new role for Grb14 in finely tuning receptor signaling and modulating thyroid cancer progression.

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“…21). Expression of the MAGE-A (melanoma-associated antigen) is also associated with aggressive tumor behavior (22,23).…”

Section: Introductionmentioning

confidence: 99%

A High-Throughput Proteomic Approach Provides Distinct Signatures for Thyroid Cancer Behavior

Cheng

Serra

Mercado

et al. 2011

Clinical Cancer Research

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Purpose: Well-differentiated thyroid cancer (WDTC) is the most frequent form of endocrine neoplasia. One of the main challenges in the management of this disease is distinguishing low-risk patients who can be treated by surgical resection of the lesion from those with a high likelihood of recurrence who need a more extensive approach, including total thyroidectomy and radioiodine ablation.Experimental Design: A tissue microarray (TMA) comprising 410 cases of WDTC was constructed with risk estimates for the following features: extrathyroidal extension, lymph node metastases, and vascular invasion. The variables examined were morphologic classification, candidate genetic, and proteomic biomarkers.Results: BRAF (Raf kinase type B) mutant carriers showed increased risk of developing invasion compared with wild-type (WT) cases. However, when classified morphologically, classic papillary thyroid carcinomas (PTC) showed much higher risk estimates for invasive features compared with follicular variant PTCs (FVPTC); within these morphologic subgroups, BRAF mutational status did not provide independent risk estimates. Staining intensities for membranous galectin-3 (Gal3), HBME-1, and CK19 and nuclear Gal3 were statistically validated as markers of aggressive behavior. Estrogen receptor beta (ERb) was overexpressed in lesions with invasive behavior. The utility of these biomarkers remained statistically significant in the FVPTC. In contrast, a different set of biomarkers proved effective in classic PTC where upregulation of cyclin D1, loss of p27, and overexpression of ERb were associated with invasive behavior.Conclusion: Different proteomic signatures validate the distinction of classic and FVPTC and provide a practical clinical mechanism to predict the thyroid cancer behavior and stratify patients for clinical management. Clin Cancer Res; 17(8); 2385-94. Ó2011 AACR.

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Expression of the melanoma-associated antigen is associated with progression of human thyroid cancer

Cited by 18 publications

References 26 publications

Human MageB2 Protein Expression Enhances E2F Transcriptional Activity, Cell Proliferation, and Resistance to Ribotoxic Stress

Human MageB2 Protein Expression Enhances E2F Transcriptional Activity, Cell Proliferation, and Resistance to Ribotoxic Stress

The insulin resistance Grb14 adaptor protein promotes thyroid cancer ret signaling and progression

A High-Throughput Proteomic Approach Provides Distinct Signatures for Thyroid Cancer Behavior

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