Expression of the melanoma antigen-encoding gene in human lung cancer

Abstract. The aim of this study was to detect differences in the expression levels of melanoma-associated antigen D4 (MAGED4) mRNA between non-small cell lung cancer (NSCLC) tissues and normal tissues, and to compare differences in the expression levels of MAGED4 in tumor patients. Patients were grouped according to age, gender, smoking history, tumor size, pathological classification, degree of lung cancer cell differentiation and presence of lymph node metastasis. The expression levels of MAGED4 were detected using real-time fluorescence quantitative PCR. MAGED4 expression was higher in squamous cell carcinomas compared to adenocarcinomas (P<0.05), in poorly differentiated tissues compared to well-differentiated tissues (P<0.05), and in patients with lymph node metastasis compared to patients without lymph node metastasis (P<0.05). MAGED4 may be used as a specific antigen for NSCLC to influence the improvement of diagnosis, prognosis and immunological therapy outcomes in lung cancer patients.

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“…These results were in accordance with the properties of other members of the MAGE gene family discovered in previous studies (15,17,18).…”

Section: Discussionsupporting

confidence: 93%

The significance of MAGED4 expression in non-small cell lung cancer as analyzed by real-time fluorescence quantitative PCR

Pang

Chen

et al. 2012

Oncology Letters

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“…A previous report has suggested that 32% of lung cancer specimens were positive to MAGE-1 antibody, however, the majority of the positive specimens showed low and heterogeneous staining (Jungbluth et al, 2000). Similarly, RT ± PCR analysis indicated that MAGE-3 gene was present in 45% lung carcinoma but the level of MAGE-3 expression was not quantitative (Yoshimatsu et al, 1998). Because of its high prevalence of expression and apparent immunogenicity, L552S represents an attractive candidate for therapeutic cancer vaccines in patients who over-express this protein.…”

Section: Discussionmentioning

confidence: 99%

L552S, an alternatively spliced isoform of XAGE-1, is over-expressed in lung adenocarcinoma

et al. 2001

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Using a combination of cDNA subtraction and microarray analysis, we report here the identi®cation and characterization of L552S, an over-expressed, alternatively spliced isoform of XAGE-1 in lung adenocarcinoma. Real-time RT ± PCR analysis shows that L552S is expressed at levels greater than 10-fold in 12 of 25 lung adenocarcinoma tumors compared with the highest expression level found in all normal tissues tested. L552S is expressed in both early and late stages of lung adenocarcinoma, but it was not detected in large cell carcinoma, small cell carcinoma, or atypical lung neuroendocrine carcinoid. The full-length cDNA for L552S comprises 770 bp and encodes a polypeptide of 160 amino acids. C-terminal 94 amino acids of L552S are identical to a cancer testis antigen, XAGE-1, found in Ewing's sarcoma. Genomic sequence analysis has revealed that L552S and XAGE-1 are alternatively spliced isoforms, and expression of both L552S and XAGE-1 isoforms are present in lung adenocarcinoma. Immunohistochemistry analysis using a nity puri®ed L552S polyclonal antibodies demonstrated speci®c nuclear staining in 10 of 12 lung adenocarcinoma samples. Furthermore, antibody responses to recombinant L552S protein were observed in seven of 17 lung pleural e usion¯uids of lung cancer patients. These results strongly imply that L552S protein is immunogenic and suggest that it might have use as a vaccine target for lung cancer. Oncogene (2001) 20, 7699 ± 7709.

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“…23 We also analyzed the expression of several known tumor-associated antigens. The MAGE-A3 40,41 and MAGE-B2 42 genes, which reportedly are cancer-testis antigens, were expressed equally in the G603L and G603AD cell lines in reverse transcriptase-polymerase chain reaction analysis (data not shown). The survivin-2B 43 gene, which reportedly is a splicing variant of survivin, also was expressed at the same level in both cell lines (data not shown).…”

Section: Discussionmentioning

confidence: 91%

A different pattern of cytotoxic T lymphocyte recognition against primary and metastatic tumor cells in a patient with nonsmall cell lung carcinoma

Takenoyama

Ichiki

et al. 2004

Cancer

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BACKGROUNDLung carcinoma represents the most frequent cause of cancer death worldwide because of tumor metastases. The objective of the current study was to analyze the immunologic response during the progress of lung carcinoma metastasis.METHODSThe authors established two tumor cell lines that were derived from primary and metastatic lesions in a patient with lung carcinoma (Patient G603). One cell line (G603L) was established from the primary lesion, and the other cell line (G603AD) was established from a metastatic lesion in the right adrenal gland 7 months after the patient underwent surgery for the primary lesion. Autologous regional lymph node lymphocytes were stimulated with CD80‐transfected G603L cells, then cytotoxic T lymphocytes (CTLs) were induced against both lung carcinoma cell lines.RESULTSBoth G603L cells and G603AD cells expressed Class I human leukocyte antigen, intracellular cell adhesion molecule 1, and lymphocyte‐associated antigen type 3 (LFA‐3), but not Fas or Fas ligand on their surfaces. By stimulation with CD80‐transfected G603L cells, 2 CTL clones (H2/17 and H2/36) were established from the bulk CTLs. CTL clone H2/17 lysed G603L cells but not G603AD cells, suggesting that the antigen recognized by CTL clone H2/17 was abrogated during the process of metastasis. In contrast, CTL clone H2/36 lysed both G603L cells and G603AD cells, indicating that the antigen recognized by CTL clone H2/36 was maintained in the tumor cells throughout tumor progression.CONCLUSIONSThe results demonstrated the possibility that some tumor‐associated antigens may be abrogated during the process of metastasis, although others are maintained. The identification of these antigens will lead to a better understanding of their immunologic role during disease progression in patients with lung carcinoma. Cancer 2005. © 2004 American Cancer Society.

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Expression of the melanoma antigen-encoding gene in human lung cancer

Cited by 36 publications

References 23 publications

The significance of MAGED4 expression in non-small cell lung cancer as analyzed by real-time fluorescence quantitative PCR

The significance of MAGED4 expression in non-small cell lung cancer as analyzed by real-time fluorescence quantitative PCR

L552S, an alternatively spliced isoform of XAGE-1, is over-expressed in lung adenocarcinoma

A different pattern of cytotoxic T lymphocyte recognition against primary and metastatic tumor cells in a patient with nonsmall cell lung carcinoma

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