Expression of the Major Neurofilament Subunit in Chicken Erythrocytes

Granger, Bruce L.; Lazarides, Elias

doi:10.1126/science.6346488

Cited by 45 publications

(23 citation statements)

References 43 publications

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“…Although SetD8 was not required for Ter119 induction in G1E-ER-GATA-1 cells grown under conventional conditions in a 2-d maturation assay, it repressed embryonic/ fetal β-like globin genes and additional genes expected to have important roles in cellular physiology. Our results establish the molecular underpinnings of a pivotal biological mechanism in which Vim (encoding Vimentin) is down-regulated during erythropoiesis as a key step in maturation-associated cytoskeletal remodeling (57). The loss of Vimentin-containing intermediate filaments has been proposed to be a prerequisite for enucleation (58).…”

Section: Discussionmentioning

confidence: 77%

Establishing a hematopoietic genetic network through locus-specific integration of chromatin regulators

DeVilbiss

Boyer

Bresnick

2013

Proc. Natl. Acad. Sci. U.S.A.

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The establishment and maintenance of cell type-specific transcriptional programs require an ensemble of broadly expressed chromatin remodeling and modifying enzymes. Many questions remain unanswered regarding the contributions of these enzymes to specialized genetic networks that control critical processes, such as lineage commitment and cellular differentiation. We have been addressing this problem in the context of erythrocyte development driven by the transcription factor GATA-1 and its coregulator Friend of GATA-1 (FOG-1). As certain GATA-1 target genes have little to no FOG-1 requirement for expression, presumably additional coregulators can mediate GATA-1 function. Using a genetic complementation assay and RNA interference in GATA-1-null cells, we demonstrate a vital link between GATA-1 and the histone H4 lysine 20 methyltransferase PR-Set7/SetD8 (SetD8). GATA-1 selectively induced H4 monomethylated lysine 20 at repressed, but not activated, loci, and endogenous SetD8 mediated GATA-1-dependent repression of a cohort of its target genes. GATA-1 used different combinations of SetD8, FOG-1, and the FOG-1-interacting nucleosome remodeling and deacetylase complex component Mi2β to repress distinct target genes. Implicating SetD8 as a context-dependent GATA-1 corepressor expands the repertoire of coregulators mediating establishment/maintenance of the erythroid cell genetic network, and provides a biological framework for dissecting the cell type-specific functions of this important coregulator. We propose a coregulator matrix model in which distinct combinations of chromatin regulators are required at different GATA-1 target genes, and the unique attributes of the target loci mandate these combinations.

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Section: Discussionmentioning

confidence: 77%

Establishing a hematopoietic genetic network through locus-specific integration of chromatin regulators

DeVilbiss

Boyer

Bresnick

2013

Proc. Natl. Acad. Sci. U.S.A.

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“…NF proteins appear in central and peripheral neurons early during development, increasing in quantity, rapidly and progressively, throughout the cells of the nervous system during neurogenesis. While in situ hybridization studies have shown the neuronal specificity of NF gene expression, non-nerve cell expression does occur (Granger and Lazarides, 1983;Bennett and DiLullo, 1985;Gorza et al, 1988). NF proteins are expressed in bovine chromafFm cells in culture (Bader et al, 1984) and in a clonal line of pheochromocytoma (PC-12) cells (Lee et al, 1982;Franke et al, 1986).…”

Section: Indexing Terms: Cytoskeleton Intermediate Filaments Neuralmentioning

confidence: 99%

Immunomicroscopy of neurofilaments in chromaffin cells of the adult bovine adrenal gland

et al. 1996

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Neurofilaments (NFs) represent a class of intermediate filaments which are highly specific for neurons. The most abundant of the native NFs is the 68 kD subunit (NF-L). Chromaffin cells of the adrenal medulla express NF subunits under culture conditions. However, NF expression in situ is questionable. It has been reported that as chromaffin cell precursors mature and begin to express catecholamine-synthesizing enzymes, their neuronal traits are extinguished and they become endocrine-like cells. This study reports that while NF-L may be lacking in the adrenal medulla of some species, NF-L immunoreactivity is clearly present in the adult bovine adrenal medulla. Immunofluorescence microscopy of bovine chromaffin cells in culture demonstrated NF immunoreactivity localized to both thin, highly ramified filaments present throughout the cell and frequently to an intensely immunofluorescent spot located near the nucleus. Double-label immunofluorescence microscopy and immunoblot analysis also demonstrated NF-L immunoreactivity in mature chromaffin cells of the bovine adrenal gland. In vitro and in situ immunofluorescence results were confirmed by immunogold and immunoperoxidase labelling, respectively. In both cases, NF-L immunoreactivity was associated with filaments in close proximity to the nucleus. Additionally, a spheroidal aggregate of immunogold-labelled NFs was seen adjacent to the nucleus in cultured cells. In conclusion, NF-L in bovine chromaffin cells demonstrates that at least one neuronal trait persists in these catecholamine-producing cells of the mature adrenal gland. In addition, this study emphasizes the fact that interspecies comparisons must be interpreted with caution, especially when attempting to formulate a generalized hypothesis.

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“…Although IF proteins of the neurofilament type are normally restricted to neurons, there are some reports on expression in non-neuronal cells as well (Granger and Lazarides, 1983). An interesting aspect of non-neuronal expression has been found in heart conduction myocytes where NF proteins are expressed together with desmin (Vitadello et al, 1990).…”

Section: Neuronal Intermediate Filaments Neurofilamentsmentioning

confidence: 99%