Expression of the <i>Hsp83</i> gene in response to diapause and thermal stress in the moth <i>Sesamia nonagrioides</i>

Gkouvitsas, Theodoros; Kontogiannatos, Dimitriοs; Kourti, Anna

doi:10.1111/j.1365-2583.2009.00922.x

Cited by 46 publications

(23 citation statements)

References 49 publications

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“…The amount of SnoHsp70 mRNA dips as S. nonagrioides enters diapause, whereas the cognate SnoHsc70 mRNA multiplies in deep diapause [112], as does mRNA encoded by the Hsp83 gene, a homologue of Hsp90 [113]. These observations suggest diapause-related roles for Hsc70 and Hsp90, perhaps in maintaining protein structure and contributing to ecdysone action.…”

Section: Introductionmentioning

confidence: 66%

Gene expression, metabolic regulation and stress tolerance during diapause

MacRae

2010

Cell. Mol. Life Sci.

192

194

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Diapause entails molecular, physiological and morphological remodeling of living animals, culminating in a dormant state characterized by enhanced stress tolerance. Molecular mechanisms driving diapause resemble those responsible for biochemical processes in proliferating cells and include transcriptional, post-transcriptional and post-translational processes. The results are directed gene expression, differential mRNA and protein accumulation and protein modifications, including those that occur in response to changes in cellular redox potential. Biochemical pathways switch, metabolic products change and energy production is adjusted. Changes to biosynthetic activities result for example in the synthesis of molecular chaperones, late embryogenesis abundant (LEA) proteins and protective coverings, all contributing to stress tolerance. The purpose of this review is to consider regulatory and mechanistic strategies that are potentially key to metabolic control and stress tolerance during diapause, while remembering that organisms undergoing diapause are as diverse as the processes itself. Some of the parameters described have well-established roles in diapause, whereas the evidence for others is cursory.

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Section: Introductionmentioning

confidence: 66%

Gene expression, metabolic regulation and stress tolerance during diapause

MacRae

2010

Cell. Mol. Life Sci.

192

194

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“…However, two large Hsps (Hsp67 and Hsp83) show the opposite pattern and are upregulated by 48h after the first metabolic rate increase. Hsps can be upregulated during periods of rapid development (Michaud et al, 1997), and Hsp83 (also commonly referred to as Hsp90) in particular shows expression patterns that track pulses of ecdysone during development in D. melanogaster (Thomas and Lengyel, 1986) and appears to be associated with molting in the moth Sesamia nonagrioides (Gkouvitsas et al, 2009).…”

Section: Release From Metabolic Suppression and Stress Bufferingmentioning

confidence: 99%

Developmental trajectories of gene expression reveal candidates for diapause termination: a key life-history transition in the apple maggot flyRhagoletis pomonella

Ragland

Egan

Feder

et al. 2011

Journal of Experimental Biology

132

127

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SUMMARYThe timing of dormancy is a rapidly evolving life-history trait playing a crucial role in the synchronization of seasonal life cycles and adaptation to environmental change. But the physiological mechanisms regulating dormancy in animals remain poorly understood. In insects, dormancy (diapause) is a developmentally dynamic state, and the mechanisms that control diapause transitions affect seasonal timing. Here we used microarrays to examine patterns of gene expression during dormancy termination: a crucial life-history transition in the apple maggot fly Rhagoletis pomonella (Walsh). This species is a model system for host race formation and ecological speciation via changes in diapause regulation of seasonality. Our goal was to pinpoint the timing of the transition from diapause to post-diapause development and to identify candidate genes and pathways for regulation of diapause termination. Samples were taken at six metabolically defined developmental landmarks, and time-series analysis suggests that release from metabolic depression coincides with preparation for or resumption of active cell cycling and morphogenesis, defining the ʻendʼ of diapause. However, marked changes in expression, including members of pathways such as Wnt and TOR signaling, also occur prior to the metabolic rate increase, electing these pathways as candidates for early regulation of diapause termination. We discuss these results with respect to generalities in insect diapause physiology and to our long-term goal of identifying mechanisms of diapause adaptation in the Rhagoletis system. Supplementary material available online at

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“…Diapause is usually limited to one life stage per organism which displays varying degrees of metabolic suppression and stress tolerance, occurring in embryos of the silkworm, Bombyx mori (Moribe et al, 2010), larvae of the moth Sesamia nonagrioides (Gkouvitsas et al, 2009a;Gkouvitsas et al, 2009b), pupae of the flesh fly Sarcophaga crassipalpis (Li et al, 2007) and adults of the mosquito Culex pipiens (Kim et al, 2010).…”

Section: *Author For Correspondence (Tmacrae@dalca)mentioning

confidence: 99%

Artemin, a Diapause-Specific Chaperone, Contributes to the Stress Tolerance of Artemia Cysts and Influences Their Release from Females

King

Toxopeus

MacRae

2014

Journal of Experimental Biology

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Females of the crustacean Artemia franciscana produce either motile nauplii or gastrula stage embryos enclosed in a shell impermeable to nonvolatile compounds and known as cysts. The encysted embryos enter diapause, a state of greatly reduced metabolism and profound stress tolerance. Artemin, a diapause-specific ferritin homolog in cysts has molecular chaperone activity in vitro. Artemin represents 7.2% of soluble protein in cysts, approximately equal to the amount of p26, a small heat shock protein. However, there is almost twice as much artemin mRNA in cysts as compared with p26 mRNA, suggesting that artemin mRNA is translated less efficiently. RNA interference employing the injection of artemin double-stranded RNA into the egg sacs of A. franciscana females substantially reduced artemin mRNA and protein in cysts. Decreasing artemin diminished desiccation and freezing tolerance of cysts, demonstrating a role for this protein in stress resistance. Knockdown of artemin increased the time required for complete discharge of a brood of cysts carried within a female from a few hours up to 4 days, an effect weakened in successive broods. Artemin, an abundant molecular chaperone, contributes to stress tolerance of A. franciscana cysts while influencing their development and/or exit from females.

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Expression of the Hsp83 gene in response to diapause and thermal stress in the moth Sesamia nonagrioides

Cited by 46 publications

References 49 publications

Gene expression, metabolic regulation and stress tolerance during diapause

Gene expression, metabolic regulation and stress tolerance during diapause

Developmental trajectories of gene expression reveal candidates for diapause termination: a key life-history transition in the apple maggot flyRhagoletis pomonella

Artemin, a Diapause-Specific Chaperone, Contributes to the Stress Tolerance of Artemia Cysts and Influences Their Release from Females

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