In this study, water and methanol extracts were obtained from sea buckthorn seeds, which have an important place in health. It was aimed to determine the DPPH, ABTS antioxidant activities and urease, collagenase enzyme inhibition activities of these extracts. The inhibitory effect of the prepared extracts on these enzymes was evaluated by in vitro method. The best DPPH and ABTS scavenging activity was observed in aqueous extracts. DPPH (IC50 = 19.34±0.49 µg/mL) and ABTS (IC50 = 7.24±0.14 µg/mL) were determined. According to DPPH results, while aqueous extract provided 25,85 % removal, the methanolic extract provided 20,44 % removal. In ABTS removal activity, while aqueous extract provided 25,55 % removal, the methanolic extract provided 18,23% removal. The lower the IC50 value, the higher the free radical scavenging power. The best urease and collagenase inhibition from sea buckthorn seed extracts was seen in the aqueous extract (IC50 = 0,23 ± 0,003μg/mL, IC50 = 2,19 ± 1,12μg/mL). Methanol extracts did not show tyrosinase and urease enzyme inhibition. This study clearly shows that sea buckthorn the aqueous extract of can be used as an alternative source of anti-urease and anti-collagenase source. Due to the important role of these enzymes in various diseases, new drugs with inducing or inhibitory effects should be developed.