Expression of the Chlamydomonas reinhardtii Sedoheptulose‐1,7‐bisphosphatase in Dunaliella bardawil leads to enhanced photosynthesis and increased glycerol production
Abstract:SummaryBioengineering of photoautotrophic microalgae into CO 2 scrubbers and producers of valueadded metabolites is an appealing approach in low-carbon economy. A strategy for microalgal bioengineering is to enhance the photosynthetic carbon assimilation through genetically modifying the photosynthetic pathways. The halotolerant microalgae Dunaliella posses an unique osmoregulatory mechanism, which accumulates intracellular glycerol in response to extracellular hyperosmotic stresses. In our study, the Calvin c… Show more
“…The overexpression of SBPase generated more total organic carbon content and increased glycerol production by about 37% under high salinity conditions (Fang et al . ).…”
Section: Improving Productivity By Metabolic Engineeringmentioning
confidence: 97%
“…The overall rate of photosynthesis of the transgenic Dunaliella was superior to the wild type, where at 2 mol l À1 NaCl, the photosynthetic efficiency (nmol O 2 per cell per lmol photons per m 2 ) and rates of CrSBP were 50-100% higher. The overexpression of SBPase generated more total organic carbon content and increased glycerol production by about 37% under high salinity conditions (Fang et al 2012).…”
Section: Improving Carbon Fixationmentioning
confidence: 99%
“…As a result, the activity of SBPase was about 80% higher in CrSBP than in the wild type. It was discovered that CrSBP growth rates at different salinities was overall slower than the wild type, yet with less variation and less sensitivity to salinity (Fang et al 2012). The overall rate of photosynthesis of the transgenic Dunaliella was superior to the wild type, where at 2 mol l À1 NaCl, the photosynthetic efficiency (nmol O 2 per cell per lmol photons per m 2 ) and rates of CrSBP were 50-100% higher.…”
Summary
A lot of research has been performed on Cyanobacteria and microalgae with the aim to produce numerous biotechnological products. However, native strains have a few shortcomings, like limitations in cultivation, harvesting and product extraction, which prevents reaching optimal production value at lowest costs. Such limitations require the intervention of genetic engineering to produce strains with superior properties. Promising advancements in the cultivation of Cyanobacteria and microalgae have been achieved by improving photosynthetic efficiency through increasing RuBisCO activity and truncation of light‐harvesting antennae. Genetic engineering has also contributed to final product extraction by inducing autolysis and product secretory systems, to enable direct product recovery without going through costly extraction steps. In this review, we summarize the different enzymes and pathways that have been targeted thus far for improving cultivation aspects, harvesting and product extraction in Cyanobacteria and microalgae. With synthetic biology advancements, genetically engineered strains can be generated to resolve demanding process issues and achieve economic practicality. This comprehensive overview of gene modifications will be useful to researchers in the field to employ on their strains to increase their yields and improve the economic feasibility of the production process.
“…The overexpression of SBPase generated more total organic carbon content and increased glycerol production by about 37% under high salinity conditions (Fang et al . ).…”
Section: Improving Productivity By Metabolic Engineeringmentioning
confidence: 97%
“…The overall rate of photosynthesis of the transgenic Dunaliella was superior to the wild type, where at 2 mol l À1 NaCl, the photosynthetic efficiency (nmol O 2 per cell per lmol photons per m 2 ) and rates of CrSBP were 50-100% higher. The overexpression of SBPase generated more total organic carbon content and increased glycerol production by about 37% under high salinity conditions (Fang et al 2012).…”
Section: Improving Carbon Fixationmentioning
confidence: 99%
“…As a result, the activity of SBPase was about 80% higher in CrSBP than in the wild type. It was discovered that CrSBP growth rates at different salinities was overall slower than the wild type, yet with less variation and less sensitivity to salinity (Fang et al 2012). The overall rate of photosynthesis of the transgenic Dunaliella was superior to the wild type, where at 2 mol l À1 NaCl, the photosynthetic efficiency (nmol O 2 per cell per lmol photons per m 2 ) and rates of CrSBP were 50-100% higher.…”
Summary
A lot of research has been performed on Cyanobacteria and microalgae with the aim to produce numerous biotechnological products. However, native strains have a few shortcomings, like limitations in cultivation, harvesting and product extraction, which prevents reaching optimal production value at lowest costs. Such limitations require the intervention of genetic engineering to produce strains with superior properties. Promising advancements in the cultivation of Cyanobacteria and microalgae have been achieved by improving photosynthetic efficiency through increasing RuBisCO activity and truncation of light‐harvesting antennae. Genetic engineering has also contributed to final product extraction by inducing autolysis and product secretory systems, to enable direct product recovery without going through costly extraction steps. In this review, we summarize the different enzymes and pathways that have been targeted thus far for improving cultivation aspects, harvesting and product extraction in Cyanobacteria and microalgae. With synthetic biology advancements, genetically engineered strains can be generated to resolve demanding process issues and achieve economic practicality. This comprehensive overview of gene modifications will be useful to researchers in the field to employ on their strains to increase their yields and improve the economic feasibility of the production process.
“…Fang et al [6] report the generation of transgenic Dunaliella bardawil expressing CrSBPase able to improve the photosynthetic performance alongw ith increased total organic carbonc ontent and the osmoticum glycerol production. In fact, as the name says, it catalyzes alternativelythe car-boxylation and oxygenation of ribulose, in ar ate ratio of approximately 3:1.…”
In this series of articles, the board members of ChemSusChem discuss recent research articles that they consider of exceptional quality and importance for sustainability. This entry features Prof. Angela Dibenedetto, who highlights the differences between natural and artificial photosynthesis, suggesting that solar chemistry may be the most appropriate terminology to describe these closely related solar-to-chemical energy conversion processes.
“…Heterologous overexpression of sedoheptulose-1,7-bisphosphatase (SBPase) of the Calvin-Benson cycle was reported to increase photosynthetic activity and carbon content in Dunaliella bardawil (Fang et al 2012 ), suggesting a limitation in biochemical reactions of the Calvin-Benson cycle that may be subjected to genetic improvement. SBPase has been identifi ed as a rate-limiting enzyme of the Calvin cycle, because it is important for regeneration of ribulose-1,5-bisphosphate, the substrate of RuBisCO.…”
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