Expression of the human sodium/iodide symporter (hNIS) in xenotransplanted human thyroid carcinoma

Smit, Johannes W. A.; Elst, J.P. Schröder van der; Karperien, Marcel; Que, Ivo; Romijn, J A; Heide, D. van der

doi:10.1055/s-2001-11019

Cited by 14 publications

(10 citation statements)

References 22 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Gene therapies based on different strategies such as p53 reintroduction, suicide gene therapy or re-expression of the sodium iodide symporter (NIS) have shown some effectiveness in animal models, but the safety and efficacy of such therapies in man are still controversial [8][9][10]. Redifferentiation therapy using retinoic acid (RA) has also been attempted in vitro and in animal models, but its clinical effectiveness has yet to be demonstrated [11,12].…”

Section: Discussionmentioning

confidence: 99%

Vitamin D3 Treatment for Locally Advanced Thyroid Cancer: A Case Report

et al. 2005

View full text Add to dashboard Cite

Abstract. There are many intricacies in the surgical treatment of locally advanced thyroid cancer, including the medical management of the remaining functional organ and any cosmetic impairments, which are sometimes very difficult to manage and eventually carry a relatively high morbidity and mortality. Here, we report on a case of a 65-year-old female with an extremely locally-advanced thyroid cancer involving both lobes of the thyroid, blood vessels, trachea and esophagus. Despite the severity of her condition, oral administration of vitamin D3 (alphacalcido) has stalled both the tumor growth and further increases of serum thyroglobulin (Tg) level, and has led to a good preservation of quality of life for the last two years. Several reports have previously demonstrated the efficacy of vitamin D3 to inhibit the proliferation of thyroid cancer cell lines in vitro, but clinical evidence has been limited so far. Therefore, this case report provides important evidence for the effectiveness of vitamin D3 therapy against advanced thyroid cancers.

show abstract

Section: Discussionmentioning

confidence: 99%

Vitamin D3 Treatment for Locally Advanced Thyroid Cancer: A Case Report

et al. 2005

View full text Add to dashboard Cite

show abstract

“…The follicular thyroid carcinoma cell line FTC133 (donated by Drs Goretzki and Simon, University of Dü sseldorf, Germany) was stably transfected with hNIS (donated by Dr Jhiang, Ohio State University, OH, USA) and named FTC133-NIS30 as described earlier (27,28). The empty vector cell-transfected cell line FTC133-V4 was also used in the experiments.…”

Section: Methodsmentioning

confidence: 99%

“…Acute effects of flavonoids on 125 I content 125 I content was measured as previously described (27,28). In brief, cells were cultured in 12-well plates and, 24 h before the addition of Na 125 I, the medium was changed to serum-free (27).…”

Section: Effects Of Flavonoids On Iodide Content Of the Cellsmentioning

confidence: 99%

See 1 more Smart Citation

Differential effects of natural flavonoids on growth and iodide content in a human Na*/I- symporter-transfected follicular thyroid carcinoma cell line

Elst

Heide

Romijn

et al. 2004

European Journal of Endocrinology

View full text Add to dashboard Cite

Objective: Natural flavonoids (plant pigments) have been shown to inhibit thyroid peroxidase (TPO) in vitro and the growth of thyroid cancer cell lines. We have studied the role of flavonoids on the iodide transport and the growth of the human follicular thyroid cancer cell line (FTC133) which was stably transfected with the human Na þ /I 2 symporter (hNIS). Design and methods: Cells were treated with flavonoids (0.5 -50 mM) for 0, 2, 4 and 6 days; 125 I content and 125 I efflux of the cells and DNA content were measured. Results: Cell growth was inhibited significantly at day 6 by most flavonoids. Eight out of ten flavonoids decreased the 125 I content of the cells at day 4. Morin did not influence the 125 I content of the cells and, surprisingly, myricetin increased the 125 I content of the cells. Kaempferol, apigenin, luteolin and F21388 decreased NIS mRNA expression after 15, 29 and 48 h; after 96 h NIS mRNA returned to normal. Conclusion: As TPO is not present in this cell line, the effects of the flavonoids on the iodide uptake are not related to organification. Myricetin was the only flavonoid studied that increased the influx and decreased the efflux of iodide. The effect of myricetin (decreased growth and increased retention of iodide) can be of therapeutic value in the radioiodide treatment of thyroid carcinoma.European Journal of Endocrinology 150 557-564

show abstract

“…(64)(65)(66) In thyroid cancer, transduction of hNIS in a follicular thyroid cancer cell line (FTC-133) induced high uptake of radioiodine in vitro and also in vivo in a xenograft model. (67) Although the transduction of the hNIS gene can induce radioiodine influx, it is followed by rapid efflux. Inhibition of iodide efflux has to be added for a therapeutic application of the hNIS gene.…”

Section: ) Sodium/iodide Symporter (Nis) Genementioning

confidence: 99%

Redifferentiation Therapy in Thyroid Cancer

Park

2002

Korean J Endocr Surg

View full text Add to dashboard Cite

Expression of the human sodium/iodide symporter (hNIS) in xenotransplanted human thyroid carcinoma

Cited by 14 publications

References 22 publications

Vitamin D3 Treatment for Locally Advanced Thyroid Cancer: A Case Report

Vitamin D3 Treatment for Locally Advanced Thyroid Cancer: A Case Report

Differential effects of natural flavonoids on growth and iodide content in a human Na*/I- symporter-transfected follicular thyroid carcinoma cell line

Redifferentiation Therapy in Thyroid Cancer

Contact Info

Product

Resources

About