Expression of the human CMP-NeuAc:GM3 α2,8-sialyltransferase (GD3 synthase) gene through the NF-κB activation in human melanoma SK-MEL-2 cells

Kang, Nam‐Young; Kim, Cheorl-Ho; Kim, Kyoung-Sook; Ko, Jeong–Heon; Lee, Jai‐Heon; Jeong, Yong-Kee; Lee, Young Choon

doi:10.1016/j.bbaexp.2007.08.001

Cited by 39 publications

(61 citation statements)

References 33 publications

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“…Actually, mechanisms for induction of GD3 expression in this system have not yet been clarified. Transcriptional factor NF-B has been reported to be one of the regulators for the expression of GD3 synthase gene in human cells (42). Tumor necrosis factor ␣ and interleukin 6 enhanced the expression of GD3 synthase in melanocytes (43), suggesting the involvement of NF-B.…”

Section: Discussionmentioning

confidence: 99%

Ganglioside GD3 Enhances Invasiveness of Gliomas by Forming a Complex with Platelet-derived Growth Factor Receptor α and Yes Kinase

Ohkawa

Momota

Kato

et al. 2015

Journal of Biological Chemistry

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Background: Roles of GD3 in gliomas are not well understood. Results: PDGF receptor ␣ was identified as a GD3-associated molecule by enzyme-mediated activation of radical sources and mass spectrometry, and its association with GD3 and Yes leads to increased invasiveness. Conclusion: GD3 enhances invasiveness by forming a molecular complex. Significance: GD3/PDGF receptor ␣⅐Yes complex is a potential target for glioma therapy.

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Section: Discussionmentioning

confidence: 99%

Ganglioside GD3 Enhances Invasiveness of Gliomas by Forming a Complex with Platelet-derived Growth Factor Receptor α and Yes Kinase

Ohkawa

Momota

Kato

et al. 2015

Journal of Biological Chemistry

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“…We have also demonstrated that the expression level of hST8Sia I gene is specifically high in human melanoma cell line SK-MEL-2 [11]. In this study, we demonstrated for the first time that RA down-regulated the expression of hST8Sia I mRNA in human melanoma cells.…”

Section: Discussionmentioning

confidence: 49%

“…Identification of RA-responsive element in nucleotide -1146 to -646 region of hST8Sia I promoter Previous studies conducted in our lab demonstrated that the region from -1146 to -646 contained putative binding sites such as c-Ets-1, AP-1, CREB and NF-κB binding sites [11,12]. To determine whether these binding sites contributed to RA-suppressed expression of hST8Sia I in SK-MEL-2 cells, four mutants (pGL3-1146/-646mtCREB, mtAP-1, mtNF-κB and mtc-Ets-1) were used, which con- tained the exact same construct as wild type pGL3-1146/-646 except that combined nucleotides within these binding sites had been changed [12].…”

Section: Transient Transfection and Reporter Assaymentioning

confidence: 99%

“…The SK-MEL-2 human melanoma cell [11] was grown in Dulbecco's modified Eagle's medium (DMEM) (WelGENE Co., Korea) supplemented with 10% heat-inactivated fetal bovine serum (FBS), 100 U/ml penicillin, and 100 μg/ml streptomycin at 37°C under 5% CO2.…”

Section: Cell Culturesmentioning

confidence: 99%

“…Recently, we have elucidated for the first time transcriptional regulation mechanism of human GD3 synthase (hST8Sia I) expression necessary for GD3 synthesis highly expressed in human melanoma cells [11].…”

Section: Introductionmentioning

confidence: 99%

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Suppression of Human GD3 Synthase (hST8Sia I) Expression Induced by Retinoic Acid in Human Melanoma SK-MEL-2 Cells

Kwon¹,

Kang²,

Lee³

2010

Journal of Life Science

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To elucidate the mechanism underlying the suppressive regulation of hST8Sia I expression in retinoic acid (RA)-induced SK-MEL-2 cells, we characterized the promoter region of the hST8Sia I gene. Functional analysis of the 5'-flanking region of the hST8Sia I gene by the transient expression method showed that the -1146 to -646 region, which contains putative binding sites for transcription factors c-Ets-1, CREB, AP-1 and NF-kB, functions as the RA-repressive promoter in SK-MEL-2 cells. Site-directed mutagenesis and ChIP analyses indicated that the NF-kB binding site at -731 to -722 is crucial for the RA-induced repression of hST8Sia I in SK-MEL-2 cells. In addition, the transcriptional activity of hST8Sia I suppressed by RA in SK-MEL-2 cells was strongly inhibited by extracellular signal-regulated protein kinase (ERK) inhibitor U0126 and protein kinase C (PKC) inhibitor GÖ6976, as determined by RT-PCR and luciferase assay of hST8Sia I promoter containing the -1146 to -646 regions. These results suggest that RA markedly modulates transcriptional regulation of hST8Sia I gene expression through the PKC/ERK signal pathway in SK-MEL-2 cells.

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Gangliosides: Structures, Biosynthesis, Analysis, and Roles in Cancer

2017

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Gangliosides are acidic glycosphingolipids containing one or more sialic acid residues. They are essential compounds at the outer leaflet of the plasma membrane, where they interact with phospholipids, cholesterol, and transmembrane proteins, forming lipid rafts. They are involved in cell adhesion, proliferation, and recognition processes, as well as in the modulation of signal transduction pathways. These functions are mainly governed by the glycan moiety, and changes in the structures of gangliosides occur under pathological conditions, particularly in neuro‐ectoderm‐derived cancers. With the progress in mass spectrometry analysis of gangliosides, their role in cancer progression can be now investigated in more detail. In this review we summarize the current knowledge on the biosynthesis of gangliosides and their role in cancers, together with the recent development of cancer immunotherapy targeting gangliosides.

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Expression of the human CMP-NeuAc:GM3 α2,8-sialyltransferase (GD3 synthase) gene through the NF-κB activation in human melanoma SK-MEL-2 cells

Cited by 39 publications

References 33 publications

Ganglioside GD3 Enhances Invasiveness of Gliomas by Forming a Complex with Platelet-derived Growth Factor Receptor α and Yes Kinase

Ganglioside GD3 Enhances Invasiveness of Gliomas by Forming a Complex with Platelet-derived Growth Factor Receptor α and Yes Kinase

Suppression of Human GD3 Synthase (hST8Sia I) Expression Induced by Retinoic Acid in Human Melanoma SK-MEL-2 Cells

Gangliosides: Structures, Biosynthesis, Analysis, and Roles in Cancer

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