Expression of the heat shock protein-27 in the adult human scalp skin and hair follicle: Hair cycle–dependent changes

α-B-Crystallin as a Tissue Marker of Epileptic Foci in Paediatric Resections

“…Experimentally, crystallin genes may be induced in E. coli bacteria 10 and transgenically in the roundworm C. elegans 11 . Heat-shock proteins are involved in protein folding, assembly and transport and the regulation of cell growth and apoptosis 12 . The functions of α-B-crystallin in this regard are suggested in the medical dictionary definition of a chaperone protein as proteins "…that play a role in the process of protein folding and translocation by binding to newly synthesized protein chains and preventing interactions with other proteins that might interfere with the intended pathway" 13 .…”

Expression pattern of p75 neurotrophin receptor protein in human scalp skin and hair follicles: Hair cycle-dependent expression

“…Second, the staining intensity was scored as 1 for weak, 2 for medium, and 3 for intense staining, following other groups. 3,7,8,11,12 Statistical analysis Statistical comparison of the protein expression values among anagen, catagen, and telogen was evaluated using analysis of variance. Calculations were done with a statistical software package (SPSS for Windows, Version 10.0, SPSS Inc, Chicago, IL).…”

Expression patterns of the glial cell line–derived neurotrophic factor, neurturin, their cognate receptors GFRα-1, GFRα-2, and a common signal transduction element c-Ret in the human skin hair follicles

“…For tyramide signal amplification labeling technique, [22][23][24] cryosections were washed in Tris-acidTween buffer (pH 7.5) followed by washing in 3% hydrogen peroxide. Sections were then incubated with lower concentrations of primary antibodies diluted in Tris-acid-blocking buffer (pH 7.2, for dilution see Table I) overnight at 48C.…”

“…The positive control specimens, for these proteins consisted of mouse brain cryosections with primary antibodies specific for all antigens to be detected. [22][23][24] Negative control. Additional sections, running in parallel but with omission of the primary antibodies, served as the negative controls.…”