Expression of the genesdualoxidase2,lipocalin 2andregenerating islet-derived 1 alphain Crohn's disease

Csillag, Cláudio; Nielsen, Ole Haagen; Vainer, Ben; Olsen, Jørgen; Dieckgraefe, Brian K.; Hendel, Jakob; Vind, Ida; Dupuy, Corinne; Nielsen, Finn Cilius; Borup, Rehannah

doi:10.1080/00365520600976266

Cited by 41 publications

(32 citation statements)

References 61 publications

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“…In conclusion, upregulation of DUOX2 seems to be linked to inflammatory bowel diseases, supporting previous reports on differential expression of NOX/DUOX family members in Crohn's disease and Helicobacter pylori-induced intestinal inflammation (Csillag et al, 2007;Rokutan et al, 2008;Szanto et al, 2005).…”

Section: Expression Of Duox2 In Intestinal Epithelial Cellssupporting

confidence: 73%

DUOX2-derived reactive oxygen species are effectors of NOD2-mediated antibacterial responses

Lipinski¹,

Till²,

Sina

et al. 2009

Journal of Cell Science

188

180

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Generation of microbicidal reactive oxygen species (ROS) is a pivotal protective component of the innate immune system in many eukaryotes. NOD (nucleotide oligomerisation domain containing protein)-like receptors (NLRs) have been implicated as phylogenetically ancient sensors of intracellular pathogens or endogenous danger signals. NOD2 recognizes the bacterial cell wall component muramyldipeptide leading to NFκB and MAPK activation via induced proximity signalling through the serine-threonine kinase RIP2. In addition to the subsequent induction of cytokines and antimicrobial peptides, NOD2 has been shown also to exert a direct antibacterial effect. Using a fluorescence-based ROS detection assay we demonstrate controlled ROS generation as an integral component of NOD2-induced signalling in epithelial cells. We demonstrate that the NAD(P)H oxidase family member DUOX2 is involved in NOD2-dependent ROS production. Coimmunoprecipitation and fluorescence microscopy were used to show that DUOX2 interacts and colocalizes with NOD2 at the plasma membrane. Moreover, simultaneous overexpression of NOD2 and DUOX2 was found to result in cooperative protection against bacterial cytoinvasion using the Listeria monocytogenes infection model. RNAi-based studies revealed that DUOX2 is required for the direct bactericidal properties of NOD2. Our results demonstrate a new role of ROS as effector molecules of protective cellular signalling in response to a defined danger signal carried out by a mammalian intracellular NLR system.

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Section: Expression Of Duox2 In Intestinal Epithelial Cellssupporting

confidence: 73%

DUOX2-derived reactive oxygen species are effectors of NOD2-mediated antibacterial responses

Lipinski¹,

Till²,

Sina

et al. 2009

Journal of Cell Science

188

180

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“…The source of epithelial ROS was identified only a few years ago, after initial discovery of prominent expression of the NOX homologs DUOX1 and DUOX2 within the lung (35), and studies on lung tissues using in situ hybridization and immunohistochemistry, which revealed the presence of DUOX1 at the apical surface of tracheobronchial epithelial cells, and DUOX2 within salivary and submucosal glands (19,79,83,84 (88,89), which is due to the presence of DUOX1 (90). Although DUOX expression in the lung is primarily localized to airway or alveolar epithelial cells, recent studies have also suggest the presence of DUOX proteins in lymphocytes (51,91).…”

Section: Nadph Oxidases Within the Airway Epithelium: Duox1 And Duox2mentioning

confidence: 99%

NADPH oxidases in lung biology and pathology: Host defense enzymes, and more

Vliet

2008

Free Radical Biology and Medicine

186

192

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The deliberate production of reactive oxygen species (ROS) by phagocyte NADPH oxidase is widely appreciated as a critical component of antimicrobial host defense. Recently, additional homologs of NADPH oxidase (NOX) have been discovered throughout the animal and plant kingdoms, which appear to possess diverse functions in addition to host defense, including cell proliferation, differentiation, and regulation of gene expression. Several of these NOX homologs are also expressed within the respiratory tract, where they participate in innate host defense as well as in epithelial and inflammatory cell signaling and gene expression, and fibroblast and smooth muscle cell proliferation, in response to bacterial or viral infection and environmental stress. Inappropriate expression or activation of NOX/DUOX during various lung pathologies suggests their specific involvement in respiratory disease. This review summarizes the current state of knowledge regarding the general functional properties of mammalian NOX enzymes, and their specific importance in respiratory tract physiology and pathology.

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“…The PCR reactions were run using the LightCycler PCR system (Roche, Manheim, Germany) as previously described. 18 To standardize the reactions, a serial 10-fold dilution of gel-purified PCR product of 1 mL was used as template in independent reactions, and the number of target molecules was calculated based on the standard curve. The number of ribosomal protein L10 transcripts (RPL10) was used for normalization between samples, as RPL10 was found constitutively and equally expressed in the microarray data from the 4 groups (calculated for probe set ID: "221989_at."…”

Section: Real-time Reverse Transcription Polymerase Chain Reactionmentioning

confidence: 99%

Transcriptional Analysis of Left-sided Colitis, Pancolitis, and Ulcerative Colitis-associated Dysplasia

Bjerrum

Nielsen

Riis

et al. 2014

Inflammatory Bowel Diseases

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Expression of the genesdualoxidase2,lipocalin 2andregenerating islet-derived 1 alphain Crohn's disease

Cited by 41 publications

References 61 publications

DUOX2-derived reactive oxygen species are effectors of NOD2-mediated antibacterial responses

DUOX2-derived reactive oxygen species are effectors of NOD2-mediated antibacterial responses

NADPH oxidases in lung biology and pathology: Host defense enzymes, and more

Transcriptional Analysis of Left-sided Colitis, Pancolitis, and Ulcerative Colitis-associated Dysplasia

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