Expression of the gene of the α-smooth muscle-actin isoform in rat liver and in rat fat-storing (ITO) cells

Ramadori, Giuliano; Veit, Th.; Schwögler, S.; Dienes, Hans‐Peter; Knittel, Thomas; Rieder, H.; Büschenfelde, K H Meyer zum

doi:10.1007/bf02899424

Cited by 272 publications

(201 citation statements)

References 35 publications

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“…The existence of ␣-SMA-positive HSCs 3 and 5 days after PHx seems to explain partly why HSCs 3 and 5 days after PHx stored only a small amount of vitamin A in their cytoplasm, because ␣-SMA is a well-known marker of activated HSCs (Ramadori et al, 1990). However, we could not observe ␣-SMA-positive HSCs in livers 1 and 7 days after PHx, although HSCs at these times also showed a low amount of vitamin A storage.…”

Section: Discussionmentioning

confidence: 99%

Vitamin A storage in hepatic stellate cells in the regenerating rat liver: With special reference to zonal heterogeneity

et al. 2005

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Under physiological conditions, hepatic stellate cells (HSCs) within liver lobules store about 80% of the total body vitamin A in lipid droplets in their cytoplasm, and these cells show zonal heterogeneity in terms of vitamin A-storing capacity. Vitamin A is essential for the growth and differentiation of cells, and it is well known that liver cells including HSCs show a remarkable growth capacity after partial hepatectomy (PHx). However, the status of vitamin A storage in HSCs in the liver regeneration is not yet known. Therefore, we conducted the present study to examine vitamin A storage in these cells during liver regeneration. Morphometry at the electron microscopic level, fluorescence microscopy for vitamin A autofluorescence, and immunofluorescence microscopy for desmin and ␣-smooth muscle actin (␣-SMA) were performed on sections of liver from male Wistar strain rats at various times after the animal had been subjected to 70% PHx. The mean area of vitamin A-storing lipid droplets per HSC gradually decreased toward 3 days after PHx, and then returned to normal within 14 days after it. However, the heterogeneity of vitamin A-storing lipid droplet area per HSC within the hepatic lobule disappeared after PHx and did not return to normal by 14 days thereafter, even though the liver volume had returned to normal. These results suggest that HSCs alter their vitamin A-storing capacity during liver regeneration and that the recovery of vitamin A homeostasis requires a much longer time than that for liver volume.

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Section: Discussionmentioning

confidence: 99%

Vitamin A storage in hepatic stellate cells in the regenerating rat liver: With special reference to zonal heterogeneity

et al. 2005

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“…Their main physiological function is vitamin A (retinoid) storage (Ramadori et al 1990;Cassiman and Roskams 2002;Ramadori and Saile 2002). In liver injury HSC (of the liver sinusoids) transform from a quiescent to an "activated" state (Ramadori et al 1990;Cassiman et al 2002;Ramadori and Saile 2002). HSC were identiWed in tissue sections by detection of the intermediate Wlament protein Desmin (Johnson et al 1992).…”

Section: Introductionmentioning

confidence: 99%

Thy-1 is expressed in myofibroblasts but not found in hepatic stellate cells following liver injury

Dudás

Mansuroglu

Batusic

et al. 2008

Histochem Cell Biol

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Thy-1 (CD90) is an adhesion molecule induced in Wbroblast populations associated with wound healing and Wbrosis. In this study the question whether Thy-1-geneexpression can be induced in hepatic stellate cells (HSC) in vivo, under conditions of liver injury or liver regeneration was addressed. Acute and chronic rat liver injury was induced by the administration of CCl 4 . For comparison, cirrhotic human liver, and rat 67% partial hepatectomy (PH) was studied as well. Thy-1-gene-expression was examined also in isolated human liver myoWbroblasts. Thy-1-mRNA expression was signiWcantly upregulated in chronic liver injury. Thy-1 + cells were detected in the periportal area of rat liver specimens in normal-, injured-and regenerativeconditions. In chronic human and rat liver injury, Thy-1 + cells were located predominantly in scar tissue. In the pericentral necrotic zone after CCl 4 -treatment, no induction of Thy-1 was found. Gremlin and Thy-1 showed comparable localization in the periportal areas. Thy-1 was not detected in either normal or capillarized sinusoids, in isolated rat HSC, and was neither inducible by inXammatory cytokines in isolated HSC, nor upregulated in treated myoWbroblasts. Based upon these data Thy-1 is not a marker of "activated" sinusoidal HSC, but it is a marker of "activated" (myo)Wbroblasts found in portal areas and in scar tissue.

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“…Thus, lipocytes in normal rat liver are characterized immunocytochemically as isolated, desmin-positive, stellate cells that are distributed throughout the hepatic lobule. Cell isolation and culture has confirmed the presence of desmin in lipocytes from normal rat liver under virtually all conditions (Tsutsumi et al, 1987;Ramadori et al, 1990;Rockey et al, 1992). Immunocytochemical studies in normal rat liver have demonstrated that desmin may also be identified in smooth muscle cells within the hepatic artery, and the central and portal vein radicles (Ballardini et al, 1988;Rockey et al , 1992).…”

Section: Cytoskeletal Phenotype Intermediate Filamentsmentioning

confidence: 93%

“…The use of antibodies directed against smooth muscle a actin has established that transitional cells from injured liver are indeed myofibroblasts (Skalli et al, 1986;Ramadori et al, 1990;Schmitt-Graff et al, 1991;Tanaka et al, 1991;Rockey et al, 1992). Early experiments demonstrated evolution of an actin-containing population of cells during focal liver and carbon tetrachloride (CCl,) injury (Ogawa et al, 1986;Ballardini et al, 1988).…”

Section: Smooth Muscle a Actinmentioning

confidence: 99%

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Cytoskeleton of liver perisinusoidal cells (lipocytes) in normal and pathological conditions

Rockey

Friedman

1992

Cell Motil. Cytoskeleton

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Expression of the gene of the α-smooth muscle-actin isoform in rat liver and in rat fat-storing (ITO) cells

Cited by 272 publications

References 35 publications

Vitamin A storage in hepatic stellate cells in the regenerating rat liver: With special reference to zonal heterogeneity

Vitamin A storage in hepatic stellate cells in the regenerating rat liver: With special reference to zonal heterogeneity

Thy-1 is expressed in myofibroblasts but not found in hepatic stellate cells following liver injury

Cytoskeleton of liver perisinusoidal cells (lipocytes) in normal and pathological conditions

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