Expression of the focal adhesion protein paxillin in lung cancer and its relation to cell motility

Salgia, Ravi; Li, Jianliang; Ewaniuk, Darren S.; Wang, Youbin; Sattler, Martin; Chen, Wen-Che; Richards, William G.; Pisick, Evan; Shapiro, Geoffrey I.; Rollins, Barrett J.; Chen, Lan Bo; Griffin, James D.; Sugarbaker, David J.

doi:10.1038/sj.onc.1202273

Cited by 82 publications

(73 citation statements)

References 32 publications

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Section: Discussionmentioning

confidence: 99%

“…Low levels or the absence of only paxillin expression have also been demonstrated in certain human lung cancers [21] and liver metastasis [1]. Cell motility, as assessed by migration membrane ruffling and the formation of lamellipodia and filopodia, has been shown to be inhibited by paxillin overexpression in lung cancer cell lines [21]. Many studies using different approaches to address the role of paxillin in the cell have produced the same conclusion that paxillin controls cell spreading and motility [23].…”

Section: Discussionmentioning

confidence: 99%

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Expression levels of the focal adhesion-associated proteins paxillin and p130^CAS in canine and feline mammary tumors

et al. 2003

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-Paxillin and p130 CAS are two adaptor proteins localized at the focal adhesions which play an important role in cell signaling, cell motility and oncogenic transformation. In this study we evaluated the levels of paxillin and p130 CAS in feline and canine mammary tumor tissues at different stages of malignancy. The results obtained by Western blotting analysis showed no significant differences in the amounts of paxillin and p130 CAS between normal and non-invasive tumor tissues. By contrast, mammary tumor tissues with the invasive phenotype showed lower levels of paxillin P < 0.01 and higher levels of p130 CAS P < 0.001 than normal tissues. The decrease P < 0.001 of the amount of paxillin and the increase P < 0.001 of p130 CAS levels were correlated with the progression stage of malignancy. Since paxillin and p130 CAS are involved in regulating cell migration, our results suggest that low levels of paxillin together with high levels of p130 CAS expression may cause certain breast cancers to be more motile and possibly more aggressive. Thus, both paxillin and p130 CAS may represent useful prognosticators of feline and canine breast cancer malignancy.paxillin / p130 CAS / mammary tumor / cat / dog

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Expression levels of the focal adhesion-associated proteins paxillin and p130^CAS in canine and feline mammary tumors

et al. 2003

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“…Paxillin and vinculin are focal adhesion proteins that are conserved in a wide variety of eukaryotes including mammals, Xenopus and Drosophila [34]. Like the mammalian homologs [35], Dictyostelium PAXB and VINA localized at dotlike structures on the basal cell membrane facing the substrate, presumably representing focal adhesions ( Figure 2).…”

Section: Discussionmentioning

confidence: 99%

Cell adhesion molecules regulate contractile ring-independent cytokinesis in Dictyostelium discoideum

2008

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To investigate the roles of substrate adhesion in cytokinesis, we established cell lines lacking paxillin (PAXB) or vinculin (VINA), and those expressing the respective GFP fusion proteins in Dictyostelium discoideum. As in mammalian cells, GFP-PAXB and GFP-VINA formed focal adhesion-like complexes on the cell bottom. paxB − cells in suspension grew normally, but on substrates, often failed to divide after regression of the furrow. The efficient cytokinesis of paxB − cells in suspension is not because of shear forces to assist abscission, as they divided normally in static suspension culture as well. Double knockout strains lacking mhcA, which codes for myosin II, and paxB or vinA displayed more severe cytokinetic defects than each single knockout strain. In mitotic wild-type cells, GFP-PAXB was diffusely distributed on the basal membrane, but was strikingly condensed along the polar edges in mitotic mhcA − cells. These results are consistent with our idea that Dictyostelium displays two forms of cytokinesis, one that is contractile ringdependent and adhesion-independent, and the other that is contractile ring-independent and adhesion-dependent, and that the latter requires PAXB and VINA. Furthermore, that paxB − cells fail to divide normally in the presence of substrate adhesion suggests that this adhesion molecule may play additional signaling roles.

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“…In lung cancers, a decrease in paxillin expression and tyrosine phosphorylation has been correlated with generation of metastatic cancer (115,222). However, upregulation of paxillin expression has been observed in proliferative prostate epithelium and correlated with increased metastatic potential (272), with increases in paxillin tyrosine phosphorylation proposed to be important (5).…”

Section: B Potential Roles In Diseasementioning

confidence: 99%

Paxillin: Adapting to Change

Brown¹,

Turner²

2004

Physiological Reviews

550

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Molecular scaffold or adaptor proteins facilitate precise spatiotemporal regulation and integration of multiple signaling pathways to effect the optimal cellular response to changes in the immediate environment. Paxillin is a multidomain adaptor that recruits both structural and signaling molecules to focal adhesions, sites of integrin engagement with the extracellular matrix, where it performs a critical role in transducing adhesion and growth factor signals to elicit changes in cell migration and gene expression.

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Expression of the focal adhesion protein paxillin in lung cancer and its relation to cell motility

Cited by 82 publications

References 32 publications

Expression levels of the focal adhesion-associated proteins paxillin and p130^CAS in canine and feline mammary tumors

Expression levels of the focal adhesion-associated proteins paxillin and p130^CAS in canine and feline mammary tumors

Cell adhesion molecules regulate contractile ring-independent cytokinesis in Dictyostelium discoideum

Paxillin: Adapting to Change

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Expression of the focal adhesion protein paxillin in lung cancer and its relation to cell motility

Cited by 82 publications

References 32 publications

Expression levels of the focal adhesion-associated proteins paxillin and p130CAS in canine and feline mammary tumors

Expression levels of the focal adhesion-associated proteins paxillin and p130CAS in canine and feline mammary tumors

Cell adhesion molecules regulate contractile ring-independent cytokinesis in Dictyostelium discoideum

Paxillin: Adapting to Change

Contact Info

Product

Resources

About

Expression levels of the focal adhesion-associated proteins paxillin and p130^CAS in canine and feline mammary tumors

Expression levels of the focal adhesion-associated proteins paxillin and p130^CAS in canine and feline mammary tumors