Expression of the facilitated glucose transporters (GLUT1 and GLUT3) by a choriocarcinoma cell line (JAr) and cytotrophoblast cells in culture

Clarson, LH; Glazier, Jocelyn D.; Sides, M.K.; Sibley, Colin P.

doi:10.1016/s0143-4004(97)80068-9

Cited by 32 publications

(23 citation statements)

References 27 publications

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“…This has not been measured before in JEG-3 and is in agreement with earlier reports on the presence of GLUT1 and GLUT3 in JAR cells (Clarson et al, 1997). Our data support the general view that GLUT1 and GLUT3 are the major, if not the only, glucose transporters expressed in transformed cells (White and McCubrey, 1995) since GLUT2, GLUT4 and GLUT5 could not be detected in a variety of human cancer tissues (Yamamoto et al, 1990).…”

Section: Discussionsupporting

confidence: 93%

Hyperglycemia regulates the glucose-transport system of clonal choriocarcinoma cellsin vitro. A potential molecular mechanism contributing to the adjunct effect of glucose in tumor therapy

et al. 1998

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Section: Discussionsupporting

confidence: 93%

Hyperglycemia regulates the glucose-transport system of clonal choriocarcinoma cellsin vitro. A potential molecular mechanism contributing to the adjunct effect of glucose in tumor therapy

et al. 1998

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“…GLUT1 is expressed primarily in erythrocytes and fetal tissues (2, 5). GLUT3, which was first found in an adult human brain (28) is widely expressed, being associated with cells showing high rates of metabolic activity (20,12). GLUT4 is a lowaffinity, insulin-regulated glucose transporter expressed in muscle and fat cells (2,19,5).…”

Section: Resultsmentioning

confidence: 99%

Multiple Hexose Transporters ofSchizosaccharomyces pombe

et al. 2000

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We have identified a family of six hexose transporter genes (Ght1 to Ght6) in the fission yeast Schizosaccharomyces pombe. Sequence homology to Saccharomyces cerevisiae and mammalian hexose transporters (Hxtp and GLUTp, respectively) and secondary-structure predictions of 12 transmembrane domains for each of the Ght proteins place them into the sugar porter subfamily within the major facilitator superfamily. Interestingly, among this sugar porter family, the emerging S. pombe hexose transporter family clusters are separate from monosaccharide transporters of other yeasts (S. cerevisiae, Kluyveromyces lactis, and Candida albicans) and of humans, suggesting that these proteins form a distinct structural family of hexose transporters. Expression of the Ght1, Ght2, Ght5, and Ght6 genes in the S. cerevisiae mutant RE700A may functionally complement its D-glucose uptake-deficient phenotype. Northern blot analysis and reverse transcription-PCR showed that among all Ght's of S. pombe, Ght5 is the most prominently expressed hexose transporter. Ght1p, Ght2p, and Ght5p displayed significantly higher specificities for D-glucose than for D-fructose. Analysis of the previously described S. pombe D-glucose transport-deficient mutant YGS-5 revealed that this strain is defective in the Ght1, Ght5, and Ght6 genes. Based on an analysis of three S. pombe strains bearing single or double mutations in Ght3 and Ght4, we conclude that the Ght3p function is required for D-gluconate transport in S. pombe. The function of Ght4p remains to be clarified. Ght6p exhibited a slightly higher affinity to D-fructose than to D-glucose, and among the Ght's it is the transporter with the highest specificity for D-fructose.Hexose transporters comprise a family of proteins involved in cellular sugar uptake. They have been well described for a variety of organisms, including bacteria, yeasts, plants, and humans. Regarding sugar metabolism, the fission yeast Schizosaccharomyces pombe shares a number of characteristic properties with the budding yeast Saccharomyces cerevisiae. Both species grow as facultative aerobes and use aerobic alcoholic fermentation in the presence of an excess of sugar (17, 13). Among the utilized carbon sources, distinct differences are present. D-Glucose, D-fructose, glycerol, and maltose are metabolized by both yeast species, with D-glucose being the preferred substrate. S. pombe cells can grow on the monosaccharide D-gluconate (23), whereas S. cerevisiae cells can utilize D-galactose and disaccharides such as sucrose (13,18). In contrast to S. cerevisiae, S. pombe can use ethanol but only in the presence of glucose (53, 54). The narrow spectrum of carbon sources accepted by S. pombe is attributed to corresponding differences in carbon metabolism. The carbon metabolism of S. pombe does not involve the glyoxylate cycle, and furthermore, some enzymes of ethanol metabolism and gluconeogenesis are not constitutively expressed (53, 13).Considering transport into the cells as the first step of the utilization of sugar, both yeast spe...

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“…We clearly showed that cytotrophoblasts of the first trimester placental villi contained GLUT3 protein. Some researchers (Clarson et al 1997;Illsley et al 1998) considered that the expression of GLUT3 protein might be confined to metabolically active, mitotic trophoblast cells, whereas non-dividing cytotrophoblast and terminally differentiated syncytiotrophoblast, characterized as metabolically less active cell types, would not express this isoform. The expression of GLUT3 protein in choriocarcinoma cell lines, which are known as mitotically active cells (Illsley et al 1998) supported this finding.…”

Section: Discussionmentioning

confidence: 99%

Do glucose transporters have other roles in addition to placental glucose transport during early pregnancy?

Korgun

Çelik-Özenci

Seval

et al. 2005

Histochem Cell Biol

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Human placenta regulates the transport of maternal molecules to the fetus. It is known that glucose transport occurs via glucose transporters (GLUTs) in the feto-placental unit. Data on the expression of GLUTs during implantation are very scarce. Moreover, the question of how the decidual leukocytes obtain the energy for their activation during implantation mechanism is still under investigation. We studied the distributions of GLUT1, GLUT3, and GLUT4 in tissue sections of first trimester pregnancies the human maternal-fetal interface. GLUT1 was present in apical microvilli of the syncytiotrophoblast, in cytotrophoblast, and in vascular patterns of the villous core, whereas GLUT3 was localized in cytotrophoblasts of placental villi and in some fetal endothelial cells. Moreover, the proliferating cells of the proximal cell columns were also immunopositive for GLUT1 and GLUT3. We did not observe any positive immunoreactivity for GLUT4 in placental and decidual tissues. Essentially, GLUT3 and also to some extent GLUT1 was present in maternal leukocytes and platelets. In conclusion, our results suggest that the glucose taken up via GLUT1 and GLUT3 from the maternal circulation might not only be needed for placental functions but also for successful implantation by trophoblast invasion, proliferation and also by having a role to support energy for maternal leukocytes.

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Expression of the facilitated glucose transporters (GLUT1 and GLUT3) by a choriocarcinoma cell line (JAr) and cytotrophoblast cells in culture

Cited by 32 publications

References 27 publications

Hyperglycemia regulates the glucose-transport system of clonal choriocarcinoma cellsin vitro. A potential molecular mechanism contributing to the adjunct effect of glucose in tumor therapy

Hyperglycemia regulates the glucose-transport system of clonal choriocarcinoma cellsin vitro. A potential molecular mechanism contributing to the adjunct effect of glucose in tumor therapy

Multiple Hexose Transporters ofSchizosaccharomyces pombe

Do glucose transporters have other roles in addition to placental glucose transport during early pregnancy?

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