1983
DOI: 10.1128/jb.153.3.1221-1227.1983
|View full text |Cite
|
Sign up to set email alerts
|

Expression of the Escherichia coli malPQ operon remains unaffected after drastic alteration of its promoter

Abstract: The malPQ operon, one of the three operons of the maltose regulon, is positively controlled by the product of gene malT. The starting point for malPQ transcription was deduced from experiments which involved a hybridization of in vivo-synthesized malPQ mRNA with adequate DNA probes, followed either by a digestion of nonhybridized DNA (S1 nuclease mapping) or by an extension of the hybridized probe (reverse transcriptase mapping). In the wild-type strain, this starting point was 37 nucleotides upstream from the… Show more

Help me understand this report

Search citation statements

Order By: Relevance

Paper Sections

Select...
1
1

Citation Types

0
16
0

Year Published

1985
1985
1998
1998

Publication Types

Select...
5
2

Relationship

1
6

Authors

Journals

citations
Cited by 76 publications
(16 citation statements)
references
References 12 publications
0
16
0
Order By: Relevance
“…of labelled DNA probe and lyophilized. The freeze-dried nucleic acids were dissolved in 30 Al of Hepes buffer (Debarbouille and Raibaud, 1983), heated at 90°C for 10 min and incubated at 42°C for 3 h. The mixture was then diluted 10-fold with S1 nuclease buffer and 500 units of SI nuclease were added. After 1 h of incubation at 37°C, the DNA was extracted twice with phenolchloroform, ethanol precipitated and electrophoresed.…”
Section: Transformation Of B Subtilis and E Colimentioning
confidence: 99%
“…of labelled DNA probe and lyophilized. The freeze-dried nucleic acids were dissolved in 30 Al of Hepes buffer (Debarbouille and Raibaud, 1983), heated at 90°C for 10 min and incubated at 42°C for 3 h. The mixture was then diluted 10-fold with S1 nuclease buffer and 500 units of SI nuclease were added. After 1 h of incubation at 37°C, the DNA was extracted twice with phenolchloroform, ethanol precipitated and electrophoresed.…”
Section: Transformation Of B Subtilis and E Colimentioning
confidence: 99%
“…The radioactive single-stranded DNA primers were then purified by polyacrylamide gel electrophoresis in the presence of urea (28). These primers were coprecipitated with 25 ,ug of the mRNA-containing preparations, obtained as described previously (8). The pellets were dissolved in 50% formamide-1 mM EDTA-0.4 M NaCl-20 mM HEPES (N-2hydroxyethylpiperazine-N'-2-ethanesulfonic acid) (pH 6.5), heated for 10 min at 75°C, and incubated for 16 h at 420C in an oven to allow hybridization.…”
mentioning
confidence: 99%
“…The pellets were dissolved in 50% formamide-1 mM EDTA-0.4 M NaCl-20 mM HEPES (N-2hydroxyethylpiperazine-N'-2-ethanesulfonic acid) (pH 6.5), heated for 10 min at 75°C, and incubated for 16 h at 420C in an oven to allow hybridization. Elongation of the DNA primer by reverse transcriptase and analysis of the products were performed as described previously (8).…”
mentioning
confidence: 99%
See 1 more Smart Citation
“…Primer extension. Primer extension was performed as described by DebarbouillC et al [29]. Thermus RNA was isolated and precipitated with the labelled primer 5'-CAAGAAGGGCC-TGAAGCTCC-3'.…”
Section: Methodsmentioning
confidence: 99%