Expression of the coxsackie and adenovirus receptor in human lung cancers

Abstract

The aim of this study is to elucidate the relation between expression of coxsackie and adenovirus receptor (CAR) and formation of lung cancer. We investigated the expression of CAR by immunohistochemistry, Western blot and real-time RT-PCR in 120 lung cancers. We found that CAR expression in tumor tissues was significantly higher than that in normal lung tissues. CAR expression had a correlation with the histological grade of lung squamous cell carcinoma; however, there was no relationship between the CAR expression and the other clinical pathological features. In vitro, silencing or overexpression of CAR could significantly inhibit or promote colony formation, cell adhesion, and invasion in A549 cells. Our findings demonstrated that CAR may play an essential role in the formation of lung cancer.

Presence of the Coxsackievirus and Adenovirus Receptor (CAR) in human neoplasms: a multitumour array analysis

“…Therefore, our data may initiate further studies in this entity. In line with prior reports, we noted abundant CAR presence in several subtypes of thyroid carcinoma (Marsee et al , 2005; Giaginis et al , 2010), in lung cancer (Wang et al , 2006; Chen et al , 2013), as well as in neuro- and medulloblastomas (Persson et al , 2006). Moreover, in agreement with previous reports we found significantly hightened CAR presence in cancers of the endometrium (Giaginis et al , 2008), ovary (Reimer et al , 2007) and cervix (Dietel et al , 2011).…”

“…Upregulation of CAR on the other hand might be suggestive of a tumour-promoting function of CAR in several other organs. In line with this hypothesis, an association has been found between high CAR expression and increased proliferation and/or invasion in endometrial, ovarian, and cervical cancers as well as in lung cancer (Brüning et al , 2005; Giaginis et al , 2008; Dietel et al , 2011; Chen et al , 2013). Furthermore, CAR has been shown to foster early carcinogenesis in ovarian and cervical cancers, with CAR-expressing cell lines displaying less sensitivity towards apoptotic stimuli (Brüning et al , 2005).…”

CAR regulates epithelial cell junction stability through control of E-cadherin trafficking

“…Our own previous work has shown that CAR expression levels affect recruitment of adherens junction proteins in MCF-7 human breast carcinoma cells without affecting basal paracellular permeability7. Moreover, CAR expression has been shown to correlate with tumour progression in some types of epithelial-derived cancer including lung cancers and in some cases to specifically correlate with reduced E-cadherin expression, a marker for EMT891011. These data suggest CAR can play important tissue specific roles in the control of cell-cell adhesion.…”

Role of long purine stretches in controlling the expression of genes associated with neurological disorders

“…This clearly indicates the mutagenic effect of PRs on GRK5 expression as one of the main causes of developing AD. Dysfunction of other PR-genes, were also reported in brain disorders such as FGF14 in mental retardation, craniosynostosis (Chen et al, 2013); FLT1 cause neuroferritinopathy (Krum et al, 2008) etc.…”