Expression of the broccoli catalase gene (BoCAT) enhances heat tolerance in transgenic Arabidopsis

Chiang, Chih-Ming; Chen, Shi-Peng; Chen, Long-Fang O.; Chiang, Ming‐Chang; Chien, Hao-Lun; Lin, Kuan–Hung

doi:10.1007/s13562-013-0210-1

Cited by 32 publications

(11 citation statements)

References 44 publications

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“…For example, introduction of KatE from E. coli into tobacco and rice resulted in high tolerance to drought and salinity in the crops, respectively [22][23][24]. Transgenic Arabidopsis containing the broccoli catalase gene BoCAT showed heat tolerance by removing the H 2 O 2 accumulated due to high temperature [25].…”

Section: Introductionmentioning

confidence: 99%

CsCAT3, a catalase gene fromCucumis sativus, confers resistance to a variety of stresses toEscherichia coli

Zhou

Liu

Yang

et al. 2017

Biotechnology & Biotechnological Equipment

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Catalase (CAT) is a key scavenging enzyme for the degradation of hydrogen peroxide (H 2 O 2 ) and plays an important role in the tolerance to diverse abiotic stresses in many different organisms. In this study, we characterized the function of a catalase gene (CsCAT3) previously isolated from Cucumis sativus in the defence against a variety of stresses. Protein alignment and phylogenetic analysis revealed that CsCAT3 was clustered in the dicot group and shared 41%-95% identity with other plant CATs. Expression analyses revealed that the expression of CsCAT3 was induced by diverse abiotic stresses such as heat, polyethylene glycol, cold and NaCl treatment, as well as by signalling molecules such as abscisic acid (ABA) and H 2 O 2 . An Escherichia coli heterologous expression system was constructed to characterize the function of CsCAT3 in vitro. Its overexpression in E. coli could increase the tolerance to heat, cold, salinity and osmotic conditions. These results indicate that CsCAT3 plays important roles in abiotic stress tolerance and that CsCAT3 confers tolerance in E. coli recombinants against abiotic stresses, which may be due to the increased activities of antioxidant enzymes that reduce the oxidative damage caused by stress conditions.

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Section: Introductionmentioning

confidence: 99%

CsCAT3, a catalase gene fromCucumis sativus, confers resistance to a variety of stresses toEscherichia coli

Zhou

Liu

Yang

et al. 2017

Biotechnology & Biotechnological Equipment

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“…One method to determine the role of candidate genes is to manipulate gene expression through transgenic transformation. Transgenic approaches have been successfully used for determining roles of other genes previously identified to be associated with improved heat tolerance in various plant species, such as heat shock proteins, heat shock transcription factors, and antioxidant enzymes [22] – [27] . To our knowledge, the in vivo roles of expansins regulating plant tolerance to heat stress have not been well documented.…”

Section: Introductionmentioning

confidence: 99%

Transgenic Tobacco Plants Overexpressing a Grass PpEXP1 Gene Exhibit Enhanced Tolerance to Heat Stress

Shi

et al. 2014

PLoS ONE

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Heat stress is a detrimental abiotic stress limiting the growth of many plant species and is associated with various cellular and physiological damages. Expansins are a family of proteins which are known to play roles in regulating cell wall elongation and expansion, as well as other growth and developmental processes. The in vitro roles of expansins regulating plant heat tolerance are not well understood. The objectives of this study were to isolate and clone an expansin gene in a perennial grass species (Poa pratensis) and to determine whether over-expression of expansin may improve plant heat tolerance. Tobacco (Nicotiana tabacum) was used as the model plant for gene transformation and an expansin gene PpEXP1 from Poa pratensis was cloned. Sequence analysis showed PpEXP1 belonged to α-expansins and was closely related to two expansin genes in other perennial grass species (Festuca pratensis and Agrostis stolonifera) as well as Triticum aestivum, Oryza sativa, and Brachypodium distachyon. Transgenic tobacco plants over-expressing PpEXP1 were generated through Agrobacterium-mediated transformation. Under heat stress (42°C) in growth chambers, transgenic tobacco plants over-expressing the PpEXP1 gene exhibited a less structural damage to cells, lower electrolyte leakage, lower levels of membrane lipid peroxidation, and lower content of hydrogen peroxide, as well as higher chlorophyll content, net photosynthetic rate, relative water content, activity of antioxidant enzyme, and seed germination rates, compared to the wild-type plants. These results demonstrated the positive roles of PpEXP1 in enhancing plant tolerance to heat stress and the possibility of using expansins for genetic modification of cool-season perennial grasses in the development of heat-tolerant germplasm and cultivars.

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“…Briefly, seeds were sterilized with 1.5 % (m/v) sodium hypochlorite, rinsed with distilleddeionized H 2 O, sown in a commercial potting soil mixture, and grown in a growth chamber under an irradiance of 300 μmol m -2 s -1 , a 14-h photoperiod, day/night temperatures of 25/20 °C, and a relative humidity of 80 %. The plants were watered three times a week and fertilized (N:P:K, 17:36:39) once a week to maintain optimal growth for 21 d. To induce a heat stress, the plants were exposed to 40 °C for 5 h (Chiang et al 2014).…”

Section: Methodsmentioning

confidence: 99%

Overexpression of the genes coding ascorbate peroxidase from Brassica campestris enhances heat tolerance in transgenic Arabidopsis thaliana

Chiang¹,

Chien²,

Chen³

et al. 2015

Biologia plant.

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Previously, the ascorbate peroxidase (APX1) activity and gene expression in Chinese cabbage (Brassica campestris, Bc) heat-tolerant cv. ASVEG2 were found to be significantly higher than in heat-sensitive cv. RN720 under a heat stress. Furthermore, BcAPX2 and BcAPX3, isoforms of BcAPX1, were cloned in this study. Our objective was to transfer BcAPX cDNA under the control of the ubiquitin promoter to Arabidopsis via Agrobacterium tumefaciens strain GV3101. We found that BcAPX genes were overexpressed in transgenic Arabidopsis, and the expression of APX, and the APX activity in transgenic lines were higher than in non-transgenic (NT) plants under high temperatures. The chlorophyll content and the germination rate were significantly higher, and the malondialdehyde content was lower in BcAPX1-3, 2-1, and 3-5 lines subjected to the heat-stress treatment than those in the NT plants. Compared to the NT plants, a lower heat-induced H 2 O 2 accumulation was detected by diaminobenzidine staining in leaves of the transgenic lines with a high APX activity indicating that the overexpression of BcAPX in Arabidopsis could enhance heat tolerance by eliminating H 2 O 2 .

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Expression of the broccoli catalase gene (BoCAT) enhances heat tolerance in transgenic Arabidopsis

Cited by 32 publications

References 44 publications

CsCAT3, a catalase gene fromCucumis sativus, confers resistance to a variety of stresses toEscherichia coli

CsCAT3, a catalase gene fromCucumis sativus, confers resistance to a variety of stresses toEscherichia coli

Transgenic Tobacco Plants Overexpressing a Grass PpEXP1 Gene Exhibit Enhanced Tolerance to Heat Stress

Overexpression of the genes coding ascorbate peroxidase from Brassica campestris enhances heat tolerance in transgenic Arabidopsis thaliana

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