Expression of the Aspergillus niger glucose oxidase gene in A. niger, A. nidulans and Saccharomyces cerevisiae

Abstract

We report the cloning of the Aspergillus niger glucose oxidase gene and its use to elevate glucose oxidase productivity in A. niger by increasing the gene dosage. In addition, the gene has been introduced into A. nidulans where it provides the novel capacity to produce glucose oxidase. A plasmid, in which DNA encoding the mature form of glucose oxidase was preceded by a Saccharomyces cerevisiae secretion signal, effected high-level production of extracellular glucose oxidase in this yeast.

Recombinant glucose oxidase from Penicillium amagasakiense for efficient bioelectrochemical applications in physiological conditions

“…It has also been overexpressed by using S. cerevisiae yeast (Rosenberg, 1993), although no detailed characterization was provided for this recombinant enzyme. However, S. cerevisiae expression system is known to produce overglycosylated GOX (Frederick et al, 1990;Whittington et al, 1990). This overglycosylation can affect the catalytic properties of the enzyme (Frederick et al, 1990;Kohen et al, 1997) and can prevent its use for the construction of bioelectrochemical devices (Alvarez-Icaza et al, 1995;Courjean et al, 2009).…”

“…It also facilitates high level of production and purification. Saccharomyces cerevisiae has been used with promising results (Frederick et al, 1990;Whittington et al, 1990). The expression by this yeast induced an overglycosylation of GOX that can interfere with the electron transfer in bioelectrochemical devices.…”

The Genus Aspergillus

“…Swart et al, (1990) have outlined a strategy to construct improved strains which carry up to four different gox overproducing mutations. Glucose oxidase overexpression has also been achieved by transforming A. niger with the glucose oxidase gene (Whittington et al, 1990;Witteveen et al, 1993). In multicopy transformants only a three-to fourfold increase in glucose oxidase activity was observed when using optimal induction conditions (Witteveen et al, 1993).…”

Expression and overproduction of glucose oxidase in Aspergillus niger

“…The god gene has been cloned, sequenced and expressed both in homologous and heterologous backgrounds (Kriechbaum et al 1989;Frederick et al 1990;Wittington et al 1990).…”