Expression of the Aspergillus aculeatus Endo- β-1,4-mannanase Encoding Gene (man1) in Saccharomyces cerevisiae and Characterization of the Recombinant Enzyme

Setati, Mathabatha Evodia; Ademark, Pia; Zyl, Willem H. van; Hahn‐Hägerdal, Bärbel; Stålbrand, Henrik

doi:10.1006/prep.2000.1371

Cited by 54 publications

(47 citation statements)

References 49 publications

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“…Notwithstanding obstacles such as hyperglycosylation and hindered secretion due to the cell wall, there is an increasing number of examples of effective secretion of heterologous proteins by S. cerevisiae. Native secretion sequences have been found sufficient to effect proper posttranslational processing and secretion of functional proteins in the case of genes originating from fungal sources, including EgI, EgII, and Xyn2 of T. reesei (367,368,521); XynC, XlnA, and Man1 of Aspergillus (129,398,611); and a glucoamylase gene. In-frame fusions to the yeast MF␣1 S secretion sequence have been used to express in S. cerevisiae saccharolytic genes from bacteria, including the end1 gene of B. fibrosolvens, the cel1 gene of R. flavefaciens, the beg1 gene of B. subtilis, and the xlnD gene of A. niger (367,368,701,702,703).…”

Section: Heterologous Cellulase Expression In Bacteriamentioning

confidence: 99%

“…Although these proteins were often extensively glycosylated, they were still efficiently secreted through the yeast cell wall into the medium (367,368). Secretion of a mannanase (Man1) of A. aculeatus was recently reported at levels corresponding to about 5% of cellular protein (611). Looking beyond saccharolytic enzymes, several mutant strains with a "supersecreting" phenotype showing substantially increased secretion of particular proteins have been isolated.…”

Section: Heterologous Cellulase Expression In Bacteriamentioning

confidence: 99%

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Microbial Cellulose Utilization: Fundamentals and Biotechnology

Lynd

Weimer

Zyl³

et al. 2002

Microbiol Mol Biol Rev

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4,020

2,868

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Fundamental features of microbial cellulose utilization are examined at successively higher levels of aggregation encompassing the structure and composition of cellulosic biomass, taxonomic diversity, cellulase enzyme systems, molecular biology of cellulase enzymes, physiology of cellulolytic microorganisms, ecological aspects of cellulase-degrading communities, and rate-limiting factors in nature. The methodological basis for studying microbial cellulose utilization is considered relative to quantification of cells and enzymes in the presence of solid substrates as well as apparatus and analysis for cellulose-grown continuous cultures. Quantitative description of cellulose hydrolysis is addressed with respect to adsorption of cellulase enzymes, rates of enzymatic hydrolysis, bioenergetics of microbial cellulose utilization, kinetics of microbial cellulose utilization, and contrasting features compared to soluble substrate kinetics. A biological perspective on processing cellulosic biomass is presented, including features of pretreated substrates and alternative process configurations. Organism development is considered for “consolidated bioprocessing” (CBP), in which the production of cellulolytic enzymes, hydrolysis of biomass, and fermentation of resulting sugars to desired products occur in one step. Two organism development strategies for CBP are examined: (i) improve product yield and tolerance in microorganisms able to utilize cellulose, or (ii) express a heterologous system for cellulose hydrolysis and utilization in microorganisms that exhibit high product yield and tolerance. A concluding discussion identifies unresolved issues pertaining to microbial cellulose utilization, suggests approaches by which such issues might be resolved, and contrasts a microbially oriented cellulose hydrolysis paradigm to the more conventional enzymatically oriented paradigm in both fundamental and applied contexts

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Section: Heterologous Cellulase Expression In Bacteriamentioning

confidence: 99%

Section: Heterologous Cellulase Expression In Bacteriamentioning

confidence: 99%

Microbial Cellulose Utilization: Fundamentals and Biotechnology

Lynd

Weimer

Zyl³

et al. 2002

Microbiol Mol Biol Rev

Self Cite

4,020

2,868

View full text Add to dashboard Cite

show abstract

“…2a,b). With the exception of the -endoglucanase I of T. reesei [31], there was little evidence of overglycosylation (data not shown), which is normally associated with foreign gene expression in S. cerevisiae [40,41].…”

Section: Enzyme Assaysmentioning

confidence: 99%

Exploitation of for the Heterologous Production of Cellulases and Hemicellulases

Rose¹,

Zyl²

2008

TOBIOTJ

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Filamentous fungi of the Aspergillus niger group are native soil saprophytic fungi. Industrial strains of this group have been extensively used for the production of plant degrading enzymes for the food and beverage, animal feed and paper-and-pulp industries. Recombinant DNA technology allows for the overproduction of these enzymes in copious amounts. The advantages and limitations of A. niger as recombinant host for enzyme production are briefly discussed. Specific attention is devoted to the overproduction of several cellulases and hemicellulases to high homogeneity in the protease-deficient strain A. niger D15. The size, temperature and pH optima of the heterologous enzymes were shown to be similar to that of their natively produced counter parts. The optimization of enzyme production in dilute sugar cane molasses, using a recombinant strain producing the xylanase II of Trichoderma reesei as example, was also demonstrated.

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“…The mannanase is purified and characterized from some types of fungi such as Agaricus bisporus, Aspergillus tamarii, A. aculeatus, A. awamori (Civas et al 1984;Christgau et al 1994;Kurakake and Komaki 2001;Setati et al 2001;Tang et al 2001), from some types of bacteria such as Bacillus agaradhaerens, B. AM001, B. stearothermophilus, B. subtilis, Clostridium thermocellum, Flavobacterium spp., Paenibacillus curdlanolyticus, Dictyoglomus thermophilum (Akino et al 1989;Talbot and Sygusch 1990;Mendoza et al 1994;Zakaria et al 1998;Gibbs et al 1999;Halstead et al 1999;Bettiol and Showell 2002;Pason et al 2006) and from some other organisms such as Lycopersicones culentum (plant), Gastropoda pulmonata (snail), Pomacea insular (snail), Littorina brevicula (mollusk) (Filichkin et al 2009;Charrier and Rouland 2001;Yamamura et al 1996). The microbial β-mannanases are commonly used in improving the quality of food, softening of pulps in paper and pulp industry, reducing the viscosity of coffee extracts, oil extraction and detergent industries (Dhawan and Kaur 2007).…”

Section: Introductionmentioning

confidence: 99%

Purification and characterization of β $$ \boldsymbol{\upbeta} $$ -mannanase from Bacillus pumilus (M27) and its applications in some fruit juices

2014

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Thermo alkaline mannanase was purified from the bacteria of Bacillus pumilus (M27) using the techniques of ammonium sulphate precipitation, DEAE-Sephadex ion exchange chromatography and Sephacryl S200 gel filtration chromatography with 111-fold and 36 % yield. It was determined that the enzyme had 2 sub-units including 35 kDa and 55 kDa in gel filtration chromatography and SDS-PAGE electrophoresis systems. The optimum pH and temperature was determined as 8 and 60°C, respectively. It was also noticed that the enzyme did not lose its activity at a wide interval such as pH 3-11 and at high temperatures such as 90°C. Additionally, the effects of some metal ions on the mannanase enzyme activity. Moreover, the clarifying efficiency of purified mannanase enzyme with some fruit juices such as orange, apricot, grape and apple was also investigated. Enzymatic treatment was carried out with 1 mL L −1 of purified mannanase for 1 h at 60°C. It was determined that the highest pure enzyme was efficient upon clarifying the apple juice at 154 % rate.

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Expression of the Aspergillus aculeatus Endo- β-1,4-mannanase Encoding Gene (man1) in Saccharomyces cerevisiae and Characterization of the Recombinant Enzyme

Cited by 54 publications

References 49 publications

Microbial Cellulose Utilization: Fundamentals and Biotechnology

Microbial Cellulose Utilization: Fundamentals and Biotechnology

Exploitation of for the Heterologous Production of Cellulases and Hemicellulases

Purification and characterization of β $$ \boldsymbol{\upbeta} $$ -mannanase from Bacillus pumilus (M27) and its applications in some fruit juices

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