Expression of the Argonaute protein PiwiL2 and piRNAs in adult mouse mesenchymal stem cells

Wu, Qirui; Ma, Qi; Shehadeh, Lina A.; Wilson, Amber; Xia, Ling-Hui; Yu, Hong; Webster, Keith A.

doi:10.1016/j.bbrc.2010.05.022

Cited by 40 publications

(31 citation statements)

References 35 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…This is consistent with studies in Drosophila GSCs and in mouse mesenchymal stem cells that demonstrate a potential role for the pathway in controlling cell division (6,36,37). Our data also suggest that the PIWI-piRNA pathway has an essential function in the two strictly somatic epithelial lineages, which is likely due to a function in regulating nontransposon genes.…”

Section: Discussionsupporting

confidence: 90%

“…For example, piwi genes are expressed in the pluripotent stem cells of planarians, sponges, and tunicates, and are required for regeneration (2). Piwi expression is also found in hematopoietic stem cells in humans, mesenchymal stem cells in mice, and somatic stem cells in cnidarians and ctenophores (3)(4)(5)(6). However, detailed analyses have been largely confined to the function of the PIWIpiRNA pathway in the germline and the gonadal somatic cells in a few model bilaterians, with a focus on transposon silencing (7).…”

mentioning

confidence: 99%

See 1 more Smart Citation

PIWI proteins and PIWI-interacting RNAs function in Hydra somatic stem cells

Juliano

Reich

Liu

et al. 2013

Proc. Natl. Acad. Sci. U.S.A.

144

167

View full text Add to dashboard Cite

PIWI proteins and their bound PIWI-interacting RNAs (piRNAs) are found in animal germlines and are essential for fertility, but their functions outside of the gonad are not well understood. The cnidarian Hydra is a simple metazoan with well-characterized stem/progenitor cells that provides a unique model for analysis of PIWI function. Here we report that Hydra has two PIWI proteins, Hydra PIWI (Hywi) and Hydra PIWI-like (Hyli), both of which are expressed in all Hydra stem/progenitor cells, but not in terminally differentiated cells. We identified ∼15 million piRNAs associated with Hywi and/or Hyli and found that they exhibit the ping-pong signature of piRNA biogenesis. Hydra PIWI proteins are strictly cytoplasmic and thus likely act as posttranscriptional regulators. To explore this function, we generated a Hydra transcriptome for piRNA mapping. piRNAs map to transposons with a 25-to 35-fold enrichment compared with the abundance of transposon transcripts. By sequencing the small RNAs specific to the interstitial, ectodermal, and endodermal lineages, we found that the targeting of transposons appears to be largely restricted to the interstitial lineage. We also identified putative nontransposon targets of the pathway unique to each lineage. Finally we demonstrate that hywi function is essential in the somatic epithelial lineages. This comprehensive analysis of the PIWI-piRNA pathway in the somatic stem/progenitor cells of a nonbilaterian animal suggests that this pathway originated with broader stem cell functionality.

show abstract

Section: Discussionsupporting

confidence: 90%

mentioning

confidence: 99%

PIWI proteins and PIWI-interacting RNAs function in Hydra somatic stem cells

Juliano

Reich

Liu

et al. 2013

Proc. Natl. Acad. Sci. U.S.A.

144

167

View full text Add to dashboard Cite

show abstract

“…A similar mechanism has also been identified in mouse hippocampus, in which piRNAs may target non-transposon genes to control spine shape [142]. Mouse Mili and piRNAs are also found to be expressed in adult mouse mesenchymal stem cells [143]. In human, HIWI (human PIWI protein) is expressed in CD34(+) hematopoietic progenitor cells [144].…”

Section: Somatic Function Of the Piwi–pirna Pathwaymentioning

confidence: 90%

PIWI proteins and their interactors in piRNA biogenesis, germline development and gene expression

Lin

2014

National Science Review

159

130

View full text Add to dashboard Cite

PIWI-interacting RNAs (piRNAs) are a complex class of small non-coding RNAs that are mostly 24–32 nucleotides in length and composed of at least hundreds of thousands of species that specifically interact with the PIWI protein subfamily of the ARGONAUTE family. Recent studies revealed that PIWI proteins interact with a number of proteins, especially the TUDOR-domain-containing proteins, to regulate piRNA biogenesis and regulatory function. Current research also provides evidence that PIWI proteins and piRNAs are not only crucial for transposon silencing in the germline, but also mediate novel mechanisms of epigenetic programming, DNA rearrangements, mRNA turnover, and translational control both in the germline and in the soma. These new discoveries begin to reveal an exciting new dimension of gene regulation in the cell.

show abstract

“…57 In addition, PiWiL2, a regulatory protein which interacts with miRNA, and its associated Piwi-interacting RNAs (piRNAs) have been shown to modulate LATS2 expression as a mechanism in regulating the cell cycle of mesenchymal stem cells. 58 Finally, LATS2 mRNA stability is also regulated by tristetraprolin (TTP), which binds AU-rich elements within the 3'UTR to destabilize LATS2 mRNA and leads to subsequent downregulation of protein expression, 59 a possible mechanism for regulating LATS2 effects on cell proliferation. Therefore, the expression of LATS2 appears to be tightly regulated by several different miRNAs and mRNA binding proteins.…”

Section: Role Of Lats As Tumor Suppressormentioning

confidence: 99%