Expression of the  2-Subunit Distinguishes Synaptic and Extrasynaptic GABAA Receptors in NG2 Cells of the Hippocampus

Passlick, Stefan; Grauer, Michael; Schäfer, Christoph; Jabs, Ronald; Seifert, Gerald; Steinhäuser, Christian

doi:10.1523/jneurosci.5562-12.2013

Cited by 44 publications

(67 citation statements)

References 66 publications

(5 reference statements)

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Single-cell RT-PCR from genetically- and physiologically-identified NG2 + cells in hippocampus of P9-12 mice and cortex of P7-11 and P21-29 mice showed considerable heterogeneity of GABA A R subunit expression (Balia et al, 2015; Passlick et al, 2013). Consistent with the in vitro studies, all α subunits except α6 were expressed to some extent; α3 and α4 were upregulated with age and α5 was downregulated.…”

Section: Receptorsmentioning

confidence: 99%

“…This is in contrast to mature neurons, in which the opening of Cl − channels typically causes membrane hyperpolarization. Experiments performed in the perforated patch configuration, which preserves the native internal Cl − concentration, revealed that GABA A -mediated currents in NG2 + cells have a reversal potential in the range of −30 to −40 mV, significantly more positive than the RMP or the reversal potential of neuronal GABA A Rs (∼ −70 mV) (Lin and Bergles, 2004; Passlick et al, 2013; Tanaka et al, 2009; Tong et al, 2009). These findings led to the conclusion that NG2 + cells maintain higher internal Cl − concentration than mature neurons.…”

Section: Receptorsmentioning

confidence: 99%

See 1 more Smart Citation

Electrophysiological properties of NG2 + cells: Matching physiological studies with gene expression profiles

2016

View full text Add to dashboard Cite

NG2+ glial cells are a dynamic population of non-neuronal cells that give rise to myelinating oligodendrocytes in the central nervous system. These cells express numerous ion channels and neurotransmitter receptors, which endow them with a complex electrophysiological profile that is unique among glial cells. Despite extensive analysis of the electrophysiological properties of these cells, relatively little was known about the molecular identity of the channels and receptors that they express. The generation of new RNA-Seq datasets for NG2+ cells has provided the means to explore how distinct genes contribute to the physiological properties of these progenitors. In this review, we systematically compare the results obtained through RNA-Seq transcriptional analysis of purified NG2+ cells to previous physiological and molecular studies of these cells to define the complement of ion channels and neurotransmitter receptors expressed by NG2+ cells in the mammalian brain and discuss the potential significance of the unique physiological properties of these cells.

show abstract

Section: Receptorsmentioning

confidence: 99%

Section: Receptorsmentioning

confidence: 99%

Electrophysiological properties of NG2 + cells: Matching physiological studies with gene expression profiles

2016

View full text Add to dashboard Cite

show abstract

“…Subsequent to recording in situ, the cytoplasm of individual astrocytes was harvested and RT-PCR was performed (for details see the electronic supplementary material and [36]). …”

Section: (D) Recordings In Situ and Single-cell Rt-pcrmentioning

confidence: 99%

Heterogeneity in expression of functional ionotropic glutamate and GABA receptors in astrocytes across brain regions: insights from the thalamus

Höft

Griemsmann

Seifert

et al. 2014

Phil. Trans. R. Soc. B

View full text Add to dashboard Cite

Astrocytes may express ionotropic glutamate and gamma-aminobutyric acid (GABA) receptors, which allow them to sense and to respond to neuronal activity. However, so far the properties of astrocytes have been studied only in a few brain regions. Here, we provide the first detailed receptor analysis of astrocytes in the murine ventrobasal thalamus and compare the properties with those in other regions. To improve voltage-clamp control and avoid indirect effects during drug applications, freshly isolated astrocytes were employed. Two sub-populations of astrocytes were found, expressing or lacking a-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid (AMPA) receptors. AMPA receptor-bearing astrocytes displayed a lower Kir current density than cells lacking the receptors. In contrast, all cells expressed GABA A receptors. Single-cell RT-PCR was employed to identify the receptor subunits in thalamic astrocytes. Our findings add to the emerging evidence of functional heterogeneity of astrocytes, the impact of which still remains to be defined.

show abstract

“…[1] unit composition of the glial GABA A receptors in the hippocampus and neocortex. In addition to various α and β isoforms, the cells also have the γ2 subunit, although its expression is confined to the postsynaptic membrane; on the other hand, extrasynaptic GABA A receptors lack this subunit [6]. In the neocortex, γ2 is downregulated during postnatal development and this change is accompanied by a loss of the GABAergic synaptic innervation of NG2 glia [7].…”

Section: Transmitter Receptorsmentioning

confidence: 99%

Neuron–glia synapses in the brain: properties, diversity and functions of NG2 glia

Steinhäuser

Dietrich

2015

E-Neuroforum

View full text Add to dashboard Cite

Although NG2 glial cells represent a frequent glial cell type in the brain, characterized by expression of the NG2 proteoglycan, the functional impact of these cells is still enigmatic. A large proportion of NG2 glia are proliferatively active throughout life. These cells express a plethora of ion channels and transmitter receptors, which enable them to detect neuronal activity. Intriguingly, NG2 glial cells receive synaptic input from glutamatergic and GABAergic neurons. Since these postsynaptic glial currents are very small, their spatial and temporal integration might play an important role. In white matter, most NG2 glial cells differentiate into oligodendrocytes and this process might be influenced through the activity of the aforementioned neuron-glia synapses. Increasing evidence suggests that the properties of NG2 glia vary across brain regions; however, the impact of this variability is not understood yet.

show abstract

Expression of the 2-Subunit Distinguishes Synaptic and Extrasynaptic GABAA Receptors in NG2 Cells of the Hippocampus

Cited by 44 publications

References 66 publications

Electrophysiological properties of NG2 + cells: Matching physiological studies with gene expression profiles

Electrophysiological properties of NG2 + cells: Matching physiological studies with gene expression profiles

Heterogeneity in expression of functional ionotropic glutamate and GABA receptors in astrocytes across brain regions: insights from the thalamus

Neuron–glia synapses in the brain: properties, diversity and functions of NG2 glia

Contact Info

Product

Resources

About