Expression of the 1-SST and 1-FFT genes and consequent fructan accumulation in Agave tequilana and A. inaequidens is differentially induced by diverse (a)biotic-stress related elicitors

Suárez-González, Edgar Martín; López, Mercedes G.; Délano‐Frier, John Paul; Gómez-Leyva, Juan Florencio

doi:10.1016/j.jplph.2013.08.002

Cited by 45 publications

(53 citation statements)

References 72 publications

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“…In vitro environment is a stress factor compared with ex vitro . It has been reported a reduction in the accumulation of sugars under abiotic stress as a way to generate mechanisms of defense against abiotic factors (Suárez-González et al, 2014). In contrast, ex vitro plant metabolism is directed to the accumulation of carbohydrates (primary metabolism).…”

Section: Discussionmentioning

confidence: 99%

Micropropagation of Agave salmiana: Means to Production of Antioxidant and Bioactive Principles

2015

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Maguey, Agave salmiana, is an important plant for the “pulque” beverage and functional food industries; however, it has several constraints for elite and homogeneous plant production. In this study, a micropropagation process was established to generate in vitro plants. The effect of the method on metabolite content and antioxidant (AOX) activity in regenerated plants was evaluated. Young germinated plantlets were micropropagated from axillary shoots using Murashige and Skoog medium supplemented with L2 vitamins, 0.04 mg/L 2,4-dichlorophenoxyacetic acid and 10 mg/L 6-benzylaminopurine. Total soluble sugars from the aqueous fraction and total phenolic acids, total saponins, and AOX activity of the methanol fraction were determined in wild-type (WT) plants, in in vitro (IN) plants, and ex vitro acclimated plants (EN). The results showed that IN plants have a 50% lower soluble sugar content compared to WT, and EN. The total phenolic acids content was at least 30% higher in micropropagated (IN) and regenerated (EN) plants compared to WT. The total saponin content in IN, and EN plants was 36 and 25 times higher compared to WT. The AOX capacity of IN plants was on average three times higher compared to other treatments. However, no correlation was found between the AOX activity and total phenolic acids or total saponins. A negative and significant correlation (r = –0.927; p = 0.003) was found between the AOX activity and the total soluble sugars content. Micropropagated plants of A. salmiana have a different phytochemical content and bioactivity after the in vitro process compared to WT plants. The micropropagation process could be used as a platform for phytochemical enhancement of Agave plants.

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Section: Discussionmentioning

confidence: 99%

Micropropagation of Agave salmiana: Means to Production of Antioxidant and Bioactive Principles

2015

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“…Fructans are linear or branched polymers that are synthesized from Suc [30], and they vary in length from trisaccharides (1-ketotriose, 6-ketotriose, and neo-ketotriose) to polysaccharides that have hundreds of Fru units [31]. Fructans, whether linear or branched, are formed by Fru molecules and often by terminal Glu [32].…”

Section: Introductionmentioning

confidence: 99%

Stem carbohydrate dynamics and expression of genes involved in fructan accumulation and remobilization during grain growth in wheat (Triticum aestivum L.) genotypes with contrasting tolerance to water stress

et al. 2017

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The genetic and physiological mechanisms underlying the relationship between water-soluble carbohydrates (WSC) and water stress tolerance are scarcely known. This study aimed to evaluate the main WSC in stems, and the expression of genes involved in fructan metabolism in wheat genotypes growing in a glasshouse with water stress (WS; 50% field capacity from heading) and full irrigation (FI; 100% field capacity). Eight wheat genotypes (five tolerant and three susceptible to water stress) were evaluated initially (experiment 1) and the two most contrasting genotypes in terms of WSC accumulation were evaluated in a subsequent experiment (experiment 2). Maximum accumulation of WSC occurred 10–20 days after anthesis. Under WS, the stress-tolerant genotype exhibited higher concentrations of WSC, glucose, fructose and fructan in the stems, compared to FI. In addition, the stress-tolerant genotype exhibited higher up-regulation of the fructan 1-fructosyltransferase B (1-FFTB) and fructan 1-exohydrolase w2 (1-FEHw2) genes, whereas the susceptible cultivar presented an up-regulation of the fructan 6-fructosyltransferase (6-SFT) and fructan 1-exohydrolase w3 (1-FEHw3) genes. Our results indicated clear differences in the pattern of WSC accumulation and the expression of genes regulating fructan metabolism between the tolerant and susceptible genotypes under WS.

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“…Fructans are classified according on the linkage between fructosyl residues and glucose in six groups: inulins, with β‐(2 → 1) glycosidic linkages, characteristic of Asteraceae such as Cichorium spp. (chicory) and Helianthus tuberosus L. (Jerusalem artichoke); levans with fructosyl residues linked by β‐(2 → 6) linkages, abundant in Poaceae such as Phleum pretense L. (timothy grass) and Lolium perenne L. (perennial ryegrass); graminans with both β‐(2 → 1) and β‐(2 → 6) linkages, found in wheat and barley, and in some members of Asparagales; inulin neoseries with β‐(2 → 1) linkages, characteristic of onion and asparagus; levan neoseries with both β‐(2 → 1) and β‐(2 → 6) linkages, found in oats and some species of Poales; and agavins with β‐(2 → 1) and β‐(2 → 6) linkages in branched graminan fructans and neo‐fructans, characteristic of the genera Agave and Dasylirion Zucc …”

Section: Biotechnological Advances In Agavementioning

confidence: 99%

“…Unfortunately, few studies are available about the factors responsible for fructans accumulation in Agave , but some reports suggest it could be related to plant age, water stress, and plant hormones associated to environmental stress responses . Regarding age, tequila production generally uses 5‐ to 8‐year‐old plants in which inflorescences begin to emerge and, according to González‐Cruz et al, 6‐year‐old A. atrovirens plants have the highest accumulation of fructans compared to 3 and 9‐year‐old plants.…”

Section: Biotechnological Advances In Agavementioning

confidence: 99%

“…6) linkages in branched graminan fructans and neo-fructans, characteristic of the genera Agave and Dasylirion Zucc. 13 Table 2 summarizes the plant families from which fructans have been isolated and are currently used in food and alternative medicine. The highest concentrations of inulin have been found in species from the plant families Asteraceae (chicory, 47.6 g/100 g FW), Amaryllidaceae (garlic, 25.7 g/100 g FW), and Asparagaceae (camassia, 22 g/100 g FW; Agave, 12 g/100 g FW).…”

Section: Agave Fructans and Their Application As Functional Foodmentioning

confidence: 99%

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Review and in silico analysis of fermentation, bioenergy, fiber, and biopolymer genes of biotechnological interest inAgaveL. for genetic improvement and biocatalysis

Tamayo‐Ordóñez

Ayil‐Gutiérrez

Tamayo-Ordóñez

et al. 2018

Biotechnology Progress

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Several of the over 200 known species of Agave L. are currently used for production of distilled beverages and biopolymers. The plants live in a wide range of stressful environments as a result of their resistance to abiotic stress (drought, salinity, and extreme temperature) and pathogens, which gives the genus potential for germplasm conservation and biotechnological applications that may minimize economic losses as a result of the global climate change. However, the limited knowledge in the genus of genome structure and organization hampers development of potential improved biotechnological applications by means of genetic manipulation and biocatalysis. We reviewed Agave and plant sequences in the GenBank NCBI database for identifying genes with biotechnological potential for fermentation, bioenergy, fiber improvement, and in vivo plant biopolymer production. Three-dimensional modeling of enzyme structures in plant accessions revealed structural differences in sucrose 1-fructosyltransferase, fructan 1-fructosyltransferase, fructan exohydrolase (1-FEH), cellulose synthase (CES), and glucanases (EGases) with possible effects in fructan, sugar, and biopolymer production. Although the coding genes of FEH and enzymes involved in biopolymer production (CES, sucrose synthase, and EGases) remain unidentified in Agave L., our results could aid isolation of such genes in Agave. By comparing nucleotide and amino acid sequences in accessions of Agave and other plants, knowledge may be gained about transcriptional regulation and enzymatic activity factors. Future study is needed of biotechnological application of Agave genes for crop breeding aided by genetic engineering and biocatalysis.

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Expression of the 1-SST and 1-FFT genes and consequent fructan accumulation in Agave tequilana and A. inaequidens is differentially induced by diverse (a)biotic-stress related elicitors

Cited by 45 publications

References 72 publications

Micropropagation of Agave salmiana: Means to Production of Antioxidant and Bioactive Principles

Micropropagation of Agave salmiana: Means to Production of Antioxidant and Bioactive Principles

Stem carbohydrate dynamics and expression of genes involved in fructan accumulation and remobilization during grain growth in wheat (Triticum aestivum L.) genotypes with contrasting tolerance to water stress

Review and in silico analysis of fermentation, bioenergy, fiber, and biopolymer genes of biotechnological interest inAgaveL. for genetic improvement and biocatalysis

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